Journal of Oceanography

, Volume 74, Issue 3, pp 277–286 | Cite as

Variability in the production of organic ligands, by Synechococcus PCC 7002, under different iron scenarios

  • Guillermo Samperio-Ramos
  • J. Magdalena Santana-Casiano
  • Melchor González-Dávila
Original Article

Abstract

Several Fe-uptake mechanisms suggest the importance of the presence of certain organic ligands in phytoplankton exudates. Here, it has been studied how Synechococcus (strain PCC 7002) acclimates to Fe-bioavailability, comparing the growth and organic exudation under two different Fe regimes. These cyanobacteria were incubated in UV-treated seawater supplemented only with major nutrients and two different iron scenarios (Low-Fe and High-Fe), without chelating agents, in order to analyze the organic ligands production. The levels of dissolved organic carbon (DOC) and two natural ligands (hydroxamic and phenolic moieties) were monitored. The responses in the organic extracellular release rates (ER), normalized per cell, were statistically analyzed considering Fe scenarios and different development stages. Growth of Synechococcus was significantly slower under Low-Fe treatment, suggesting that these cultures were iron limited compared to those flourished with higher levels of iron in the medium. Although the concentration of DOC increased to 127.13 ± 8.38 and 150.51 ± 8.59 μmol C L−1 under Low-Fe and High-Fe conditions, respectively, no-significant variations were found in the DOCER, among growth phases and iron bioavailability scenarios. Under High-Fe conditions, the production of hydroxamic ligands was inhibited, while the extracellular release rate of phenolic compounds decreased, regarding to Low-Fe conditions. Growth phases of Synechococcus also modified the extracellular release rates both of hydroxamic and phenolic moieties. The present study, therefore, demonstrates that iron availability and growth stages might be key parameters in regulating the release performance of extracellular Fe-specific organic ligands by cyanobacteria.

Keywords

Synechococcus Iron Extracellular release Organic ligands Cyanobacteria Fe-bioavailability 

Notes

Acknowledgements

This study received supported from EACFe Project (CTM2014-52342-P) of the Ministerio de Economía y Competitividad of Spain. G. S. R. participation was supported by the Grant BES-2011-051448 of the Ministerio de Economía y Competitividad. The authors thank Dr. Javier Aristegui and IOCAG for the measurements of dissolved organic carbon. We gratefully acknowledge the comments and suggestions of the anonymous reviewers.

Supplementary material

10872_2017_457_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© The Oceanographic Society of Japan and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Guillermo Samperio-Ramos
    • 1
  • J. Magdalena Santana-Casiano
    • 1
  • Melchor González-Dávila
    • 1
  1. 1.Instituto de Oceanografía y Cambio GlobalUniversidad de Las Palmas de Gran CanariaLas PalmasSpain

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