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Terahertz-infrared spectroscopy of Shewanella oneidensis MR-1 extracellular matrix

Abstract

Employing optical spectroscopy we have performed a comparative study of the dielectric response of extracellular matrix and filaments of electrogenic bacteria Shewanella oneidensis MR-1, cytochrome c, and bovine serum albumin. Combining infrared transmission measurements on thin layers with data of the terahertz spectra, we obtain the dielectric permittivity and AC conductivity spectra of the materials in a broad frequency band from a few cm−1 up to 7000 cm−1 in the temperature range from 5 to 300 K. Strong absorption bands are observed in the three materials that cover the range from 10 to 300 cm−1 and mainly determine the terahertz absorption. When cooled down to liquid helium temperatures, the bands in Shewanella oneidensis MR-1 and cytochrome c reveal a distinct fine structure. In all three materials, we identify the presence of liquid bound water in the form of librational and translational absorption bands at ≈ 200 and ≈ 600 cm−1, respectively. The sharp excitations seen above 1000 cm−1 are assigned to intramolecular vibrations.

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Notes

  1. The formal amount of water in EMF is higher that the one in CytC and BSA. However, the water in our samples is in three different states: bulk, loosely bound and bound. We assume, that phenomena described here are related to the behavior of loosely bound water, the amount of which is lower in EMF. For more details, see [20]

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Acknowledgements

This work was supported by the Ministry of Education and Science of the Russian Federation (Projects N3.9896.2017/BY, 5-100) and by MIPT visiting professors grant. The authors acknowledge K.A. Motovilov for fruitful discussions. We acknowledge discussions with V.I. Borshchevskiy, V.I. Gordelii, Yu. Feldman, V.V. Lebedev, S. Tretiak, G.A. Tsirlina, A. Zhugayevych.

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T.A.V. and B.P.G. designed the research; K.V.S. and T.A.V. performed cultivation of Shewanella oneidensis MR-1, A.K.G. prepared EMF, Z.V.G., E.S.Z, V.G., K.V.S. and A.K.G. performed research; Z.V.G., E.S.Z. and V.G. analyzed data; B.P.G and M.D. wrote the paper.

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Correspondence to B. P. Gorshunov.

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Gagkaeva, Z.V., Zhukova, E.S., Grinenko, V. et al. Terahertz-infrared spectroscopy of Shewanella oneidensis MR-1 extracellular matrix. J Biol Phys 44, 401–417 (2018). https://doi.org/10.1007/s10867-018-9497-4

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Keywords

  • Shewanella oneidensis
  • Conductivity mechanism
  • Terahertz spectroscopy
  • Infrared spectroscopy