Long non-coding RNA (lncRNA) AGAP2-AS1 acts as an oncogene in several types of cancers. However, the role and mechanism of AGAP2-AS1 in papillary thyroid carcinoma (PTC) remain unclear. Thus, in this study, we aimed to explore the role of AGAP2-AS1 in PTC. Our results showed that AGAP2-AS1 was significantly upregulated in PTC tissues. Knockdown of AGAP2-AS1 inhibited the proliferation, migration and invasion of PTC cells. In vivo experiment showed that AGAP2-AS1 knockdown inhibited the tumorigenesis of PTC. MiR-628-5p was found to act as a target miRNA of AGAP2-AS1 in PTC. The expression level of miR-628‐5p in PTC tissues was negatively associated with that of AGAP2-AS1. Inhibition of miR-628-5p attenuated the effects of AGAP2-AS1 knockdown on PTC. Moreover, miR-628-5p directly bound to the 3’UTR of KLF12 and inhibited the expression of KLF12. Knockdown of KLF12 enhanced the inhibitory effects of miR-628-5p on PTC cell proliferation and metastasis. In conclusion, these findings indicated that AGAP2-AS1 exerted an oncogenic role in PTC progression and metastasis. The effects of AGAP2-AS1 might be mediated by the regulation of miR-628-5p/KLF12 axis.
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This work was supported by the Hospital Fund of the First Affiliated Hospital of Xi’an Jiaotong University (No.2019ZYTS-04), Xi’an Science and Technology Project (No. 2019114613YX001SF04(5)), the Hospital Clinical Research Project of the First Affiliated Hospital of Xi’an Jiaotong University ( XJTU1AF-CRF-2020-014), The Special Reward Fund Project for Technological Transformation of Small and Medium-sized Enterprises in Shaanxi Province (2020, Xiaobao Yao), Key Research and Development Plan of Natural Science in Shaanxi Province (No. 2017SF-151), and the Basic Natural Science Research Program of Shaanxi Province (No. 2017JM8072).
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Xu, C., Shao, Y., Liu, J. et al. Long non‐coding RNA AGAP2-AS1 promotes proliferation and metastasis in papillary thyroid cancer by miR-628-5p/KLF12 axis. J Bioenerg Biomembr (2021). https://doi.org/10.1007/s10863-021-09879-3
- Papillary thyroid carcinoma (PTC)
- Long non‐coding RNA (lncRNA)