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Deafferentation-induced alterations in mitral cell dendritic morphology in the adult zebrafish olfactory bulb

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Abstract

The removal of afferent input to the olfactory bulb by both cautery and chemical olfactory organ ablation in adult zebrafish results in a significant decrease in volume of the ipsilateral olfactory bulb. To examine the effects of deafferentation at a cellular level, primary output neurons of the olfactory bulb, the mitral cells, were investigated using retrograde tract tracing with fluorescent dextran using ex vivo brain cultures. Morphological characteristics including the number of major dendritic branches, total length of dendritic branches, area of the dendritic arbor, overall dendritic complexity, and optical density of the arbor were used to determine the effects of deafferentation on mitral cell dendrites. Following 8 weeks of permanent deafferentation there were significant reductions in the total length of dendritic branches, the area of the dendritic arbor, and the density of fine processes in the dendritic tuft. With 8 weeks of chronic, partial deafferentation there were significant reductions in all parameters examined, including a modified Sholl analysis that showed significant decreases in overall dendritic complexity. These results show the plasticity of mitral cell dendritic structures in the adult brain and provide information about the response of these output neurons following the loss of sensory input in this key model system.

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Acknowledgements

We are grateful to the Western Michigan University Biological Imaging Center for the use of the confocal microscope and Natalie K. Hamilton for her technical assistance.

Funding

Supported by National Institutes of Health-NIDCD grants #04262 and #011137 (CBJ).

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Authors and Affiliations

  1. Department of Biological Sciences, Western Michigan University, 1903 W. Michigan Ave, Kalamazoo, MI, 49008-5410, USA

    Joanna M. Pozzuto, Cynthia L. Fuller & Christine A. Byrd-Jacobs

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  1. Joanna M. Pozzuto
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  2. Cynthia L. Fuller
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  3. Christine A. Byrd-Jacobs
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Correspondence to Christine A. Byrd-Jacobs.

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Pozzuto, J.M., Fuller, C.L. & Byrd-Jacobs, C.A. Deafferentation-induced alterations in mitral cell dendritic morphology in the adult zebrafish olfactory bulb. J Bioenerg Biomembr 51, 29–40 (2019). https://doi.org/10.1007/s10863-018-9772-x

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  • DOI: https://doi.org/10.1007/s10863-018-9772-x

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