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Journal of Bioenergetics and Biomembranes

, Volume 42, Issue 6, pp 473–481 | Cite as

Functioning of the mitochondrial ATP-dependent potassium channel in rats varying in their resistance to hypoxia. Involvement of the channel in the process of animal’s adaptation to hypoxia

  • Galina D. Mironova
  • Maria I. Shigaeva
  • Elena N. Gritsenko
  • Svetlana V. Murzaeva
  • Olga S. Gorbacheva
  • Elena L. Germanova
  • Ludmila D. Lukyanova
Article

Abstract

The mechanism of tissue protection from ischemic damage by activation of the mitochondrial ATP-dependent K+ channel (mitoKATP) remains unexplored. In this work, we have measured, using various approaches, the ATP-dependent mitochondrial K+ transport in rats that differed in their resistance to hypoxia. The transport was found to be faster in the hypoxia-resistant rats as compared to that in the hypoxia-sensitive animals. Adaptation of animals to the intermittent normobaric hypoxia increased the rate of transport. At the same time, the intramitochondrial concentration of K+ in the hypoxia-sensitive rats was higher than that in the resistant and adapted animals. This indicates that adaptation to hypoxia stimulates not only the influx of potassium into mitochondria, but also K+/H+ exchange. When mitoKATP was blocked, the rate of the mitochondrial H2O2 production was found to be significantly higher in the hypoxia-resistant rats than that in the hypoxia-sensitive animals. The natural flavonoid-containing adaptogen Extralife, which has an evident antihypoxic effect, increased the rate of the mitochondrial ATP-dependent K+ transport in vitro and increased the in vivo tolerance of hypoxia-sensitive rats to acute hypoxia 5-fold. The involvement of the mitochondrial K+ transport in the mechanism of cell adaptation to hypoxia is discussed.

Keywords

Mitochondrial ATP-dependent K+ channel K+/H+ exchange ROS Hypoxia Adaptation Flavonoid-containing adaptogen 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Galina D. Mironova
    • 1
    • 3
  • Maria I. Shigaeva
    • 1
  • Elena N. Gritsenko
    • 1
  • Svetlana V. Murzaeva
    • 1
  • Olga S. Gorbacheva
    • 1
    • 3
  • Elena L. Germanova
    • 2
  • Ludmila D. Lukyanova
    • 2
  1. 1.Institute of Theoretical and Experimental Biophysics RASPushchinoRussia
  2. 2.Institute of General Pathology and Pathophysiology RAMSMoscowRussia
  3. 3.Pushchino State UniversityPushchinoRussia

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