Advertisement

Journal of Bioenergetics and Biomembranes

, Volume 42, Issue 1, pp 47–53 | Cite as

Study on the effects of chloride depletion on photosystem II using different chloride depletion methods

  • Anjana Jajoo
  • Sonal Mathur
  • Pooja Mehta
  • Miho Yoshoika
  • Suleyman I. Allakhverdiev
  • Yasusi Yamamoto
Article
  • 76 Downloads

Abstract

Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.

Keywords

Chloride Oxygen evolving complex EPR Photosystem II 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allakhverdiev SI, Shafiev MA, Klimov VV (1986) Effect of reversible extraction of manganese on photooxidation of chlorophyll P680 in photosystem II preparations. Photobiochem Photobiophys 12:61–65Google Scholar
  2. Babani F, Lichtenthaler HK (1996) Light-induced and age dependent development of chloroplasts in etiolated barley leaves as visualized by determination of photosynthetic pigments, CO2 assimilation rates and different kinds of chlorophyll fluorescence ratios. J Plant Physiol 148:555–566Google Scholar
  3. Babcock GT, Sauer K (1975) A rapid, light-induced transient in electron paramagnetic resonance signal II activated upon inhibition of photosynthetic oxygen evolution. Biochim Biophys Acta 376:315–328CrossRefGoogle Scholar
  4. Coleman W, Govindjee, Gutowsky HS (1988) The effect of chloride on thermal inactivation of oxygen evolution. Photosynth Res 16:261–276CrossRefGoogle Scholar
  5. Congming L, Vonshak A (1999) Characterization of PSII photochemistry in salt-adapted cells of cyanobacterium Spirulina platensis. New Phytol 141:231–239CrossRefGoogle Scholar
  6. Dekker JP, Van Gorkam HJ, Brok M, Ouwehand L (1984) Optical characterization of photosystem II electron donors. Biochim Biophys Acta 764:301–309CrossRefGoogle Scholar
  7. Govindjee (1995) Sixty-three years since Kautsky: chlorophyll a fluorescence. Aust J Plant Physiol 22:131–160CrossRefGoogle Scholar
  8. Guskov A, Kern J, Gabdulkhakov A, Broser M, Zouni A, Saenger W (2009) Cyanobacterial photosystem II at 2.9 Ǻ resolution and the role of quinines, lipids, channels and chloride. Nature Structural and molecular Biology 16:334–342CrossRefGoogle Scholar
  9. Havaux M, Lannoye R (1985) In vivo chlorophyll fluorescence and delayed light emission as rapid screening techniques for stress tolerance in crop plants. Z Pflanzen 95:1–13Google Scholar
  10. Hoganson CW, Babcock GT (1988) Electron transfer events near the reaction center in O2-evolving photosystem II preparations. Biochemistry 27:5848–585CrossRefGoogle Scholar
  11. Homann PH (1988) Chloride relations of photosystem II membrane preparations depleted of, and resupplied with, their 17 and 23 kDa extrinsic polypeptides. Photosynth Res 15:205–220CrossRefGoogle Scholar
  12. Ke B (2001) Photosynthesis Photobiochemistry and Photobiophysics. Series: Advances in Photosynthesis and Respiration, v. 10. Kluwer Academic Publishers, Netherlands, pp 365–375Google Scholar
  13. Klimov VV, Allakhverdiev SI, Shuvalov VA, Krasnovsky AA (1982) Effect of extraction and re-addition of manganese on light reactions of photosystem II preparations. FEBS Lett 148:307–312CrossRefGoogle Scholar
  14. Kok B, Forbush B, McGloin MP (1970) Cooperation of changes in photosynthetic O2 evolution-I. A linear four step mechanism. Photochem Photobiol 11:457–475CrossRefGoogle Scholar
  15. Kuwabara T, Murata N (1982) Inactivation of photosynthetic oxygen evolution and concomitant release of three polypeptides from photosystem II particles of spinach chloroplasts. Plant Cell Physiol 23:533–539Google Scholar
  16. Olesen K, Andreasson LE (2003) The function of the chloride in photosynthetic oxygen evolution. Biochemistry 42:2025–2035CrossRefGoogle Scholar
  17. Ono T, Zimmermann JL, Inoue Y, Rutherford AW (1986) EPR evidence for a modified S-state transition in chloride-depleted photosystem II. Biochim Biophys Acta 851:193–201CrossRefGoogle Scholar
  18. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
  19. Sonneveld A, Rademaker H, Duysens LNM (1979) Chlorophyll a fluorescence as a monitor of nanosecond reduction of the photooxidized primary donor P680+ of photosystem II. Biochim Biophys Acta 548:536–551CrossRefGoogle Scholar
  20. Strasser RJ, Srivastava A, Tsimilli-Michael M (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a Fluorescence: A Signature of Photosynthesis. Series of Advances in Photosynthesis and Respiration, vol 19. Kluwer Academic Publishers, Netherlands, pp 321–362Google Scholar
  21. Tiwari A, Jajoo A, Bharti S, Mohanty P (2007) Differential response of chloride binding sites to elevated temperature: a comparative study in spinach thylakoids and PSII-enriched membranes. Photosynth Res 93:123–132CrossRefGoogle Scholar
  22. Wincencjusz H, Allakhverdiev SI, Klimov VV, Van Gorkom HJ (1996) Bicarbonate reversible formate inhibition at the donor side of photosystem II. Biochim Biophys Acta 1273:1–3CrossRefGoogle Scholar
  23. Wydrzynski TJ (1982) Oxygen evolution in photosynthesis. In: Govindjee (ed) Photosynthesis: Energy conversion by plants and bacteria, vol. 1. Academic, New York, pp 469–506Google Scholar
  24. Yamamoto Y, Sakuma S, Shen JR (2004) Isolation and characterization of photosystem II enriched membranes and the oxygen evolving complex subunits proteins from higher plants. In: Carpentier R (ed) Methods in Molecular Biology. Humana Press, Totowa, NJ, pp 29–36Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Anjana Jajoo
    • 1
  • Sonal Mathur
    • 1
  • Pooja Mehta
    • 1
  • Miho Yoshoika
    • 2
  • Suleyman I. Allakhverdiev
    • 3
  • Yasusi Yamamoto
    • 2
  1. 1.School of Life SciencesDevi Ahilya UniversityIndoreIndia
  2. 2.Natural Science and TechnologyOkayama UniversityOkayamaJapan
  3. 3.Institute of Basic Biological ProblemsPushchinoRussia

Personalised recommendations