Retinoic acid-induced testosterone production and retinoylation reaction are concomitant and exhibit a positive correlation in Leydig (TM-3) cells
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Retinoic acid (RA) exerts diverse biological effects in the control of cell growth in embryogenesis and oncogenesis. The effects of RA are thought to be mediated by the nuclear retinoid receptors; however, not all the effects of RA can be explained by the nuclear receptor pathways. Indeed, retinoylation is another mechanism of action elicited by RA. In growing TM-3 Leydig cell cultures, the extent of retinoylation depends in a saturable manner on the initial concentration of 3H-RA, time and cell number. In addition, dose-response curves for RA-induced testosterone production and retinoylation are concomitant and exhibit a positive correlation. In the present study we demonstrate that RA is able to influence a retinoylation reaction on protein(s) probably involved on steroidogenesis.
KeywordsRetinoic acid Testosterone Retinoylation TM-3 cells Protein
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- Breitman TR, Takahashi N (1996) Biochem Soc Trans 24:723–727Google Scholar
- De Luca ML (1991) FASEB J 5:2924–2933Google Scholar
- Delia D, Aiello A, Lombardi L, Pelicci PG, Grignani F, Formelli F, Menard S, Costa A, Veronesi U, Pierotti MA (1993) Cancer Res 53:6036–6041Google Scholar
- Genchi G, Olson JA (2001) Biochim Biophys Acta 1530:146–154Google Scholar
- Myhre AM, Takahashi N, Blomhoff R, Breitman TR, Norum KR (1996) J Lipid Res 37:1971–1977Google Scholar
- Renstrom B, DeLuca HF (1989) Biochim Biophys Acta 998:69–74Google Scholar
- Sporn MB, Roberts AB, Goodman OS (1994) The Retinoids: Biology, Chemistry and Medicine, 2nd edn. Raven Press, New YorkGoogle Scholar
- Takahashi N, Breitman TR (1989) J Biol Chem 264:5159–5163Google Scholar
- Takahashi N, Breitman TR (1990) J Biol Chem 265:19158–19162Google Scholar
- Takahashi N, Breitman TR (1994) J Biol Chem 269:5913–5917Google Scholar
- Wada M, Fukui T, Kubo Y, Takahashi N (2001) J Biochem 130:457–463Google Scholar