Journal of Bioenergetics and Biomembranes

, Volume 40, Issue 2, pp 111–115 | Cite as

Retinoic acid-induced testosterone production and retinoylation reaction are concomitant and exhibit a positive correlation in Leydig (TM-3) cells

  • Paola Tucci
  • Erika Cione
  • Giuseppe Genchi


Retinoic acid (RA) exerts diverse biological effects in the control of cell growth in embryogenesis and oncogenesis. The effects of RA are thought to be mediated by the nuclear retinoid receptors; however, not all the effects of RA can be explained by the nuclear receptor pathways. Indeed, retinoylation is another mechanism of action elicited by RA. In growing TM-3 Leydig cell cultures, the extent of retinoylation depends in a saturable manner on the initial concentration of 3H-RA, time and cell number. In addition, dose-response curves for RA-induced testosterone production and retinoylation are concomitant and exhibit a positive correlation. In the present study we demonstrate that RA is able to influence a retinoylation reaction on protein(s) probably involved on steroidogenesis.


Retinoic acid Testosterone Retinoylation TM-3 cells Protein 


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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Dipartimento Farmaco-Biologico, Laboratorio di Biochimica, Edificio PolifunzionaleUniversità della CalabriaRendeItaly
  2. 2.Dipartimento Farmaco-BiologicoUniversità della CalabriaRendeItaly

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