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Journal of Bioenergetics and Biomembranes

, Volume 40, Issue 1, pp 35–44 | Cite as

Ethanol withdrawal provokes mitochondrial injury in an estrogen preventable manner

  • Marianna E. Jung
  • Liang-Jun Yan
  • Michael J. Forster
  • James W. Simpkins
Article

Abstract

We investigated whether ethanol withdrawal (EW) oxidizes mitochondrial proteins and provokes mitochondrial membrane swelling and whether estrogen deprivation contributes to this problem. Ovariectomized female rats with or without 17β-estradiol (E2)-implantation received a control diet or a liquid ethanol diet (6.5%) for 5 weeks and were sacrificed during EW. Protein oxidation was assessed by measuring carbonyl contents and was visualized by immunochemistry. Mitochondrial membrane swelling as an indicator of mitochondrial membrane fragility was assessed by monitoring absorbance at 540 nm and was compared with that of male rats. Compared to the control diet group and ovariectomized rats with E2-implantation, ovariectomized rats without E2-implantation showed higher carbonylation of mitochondrial proteins and more rapid mitochondrial membrane swelling during EW. Such rapid mitochondrial membrane swelling was comparable to that of male rats undergoing EW. These findings demonstrate that EW provokes oxidative injury to mitochondrial membranes in a manner that is exacerbated by estrogen deprivation.

Keywords

Ethanol withdrawal Mitochondrial membrane swelling Oxidation Protein carbonyls 17β-estradiol 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Marianna E. Jung
    • 1
  • Liang-Jun Yan
    • 1
  • Michael J. Forster
    • 1
  • James W. Simpkins
    • 1
  1. 1.Department of Pharmacology and NeuroscienceUniversity of North Texas Health Science Center at Fort WorthFort WorthUSA

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