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Interactions Between Vacuolar H+-ATPases and Microfilaments in Osteoclasts

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Vacuolar H+-ATPases (V-ATPases) are transported from cytosolic compartments to the ruffled plasma membrane of osteoclasts as they activate to resorb bone. Transport of V-ATPases is essential for bone resorption, and is associated with binding interactions between V-ATPases and microfilaments that are mediated by an actin-binding site in subunit B. This site is contained within 44 amino acids in the amino terminal domain, and requires a sequence motif that resembles an actin-binding motif found in mammalian profilin 1. Small alterations in the profilin-like sequence disrupt the actin-binding activity of subunit B. The interaction between V-ATPases and microfilaments in osteoclasts is regulated in response to changes in phosphatidylinositol-3 kinase activity. During internalization of V-ATPases from the plasma membrane of osteoclasts after a cycle of resorption, V-ATPases bind microfilaments that are in podosomes, dynamic actin-based structures, also present in metastatic cancer cells. Studies are ongoing to establish the physiological role of the microfilament-binding activity of subunit B in osteoclasts and in other cells.

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Authors and Affiliations

  1. Department of Orthodontics, University of Florida College of Dentistry, Gainesville, Florida

    L. Shannon Holliday, I. Rita Hurst & Jian Zuo

  2. Malcom Randall VA Medical Center, Gainesville, Florida

    Michael R. Bubb

  3. University of Connecticut Health Science Center, Farmington, Connecticut

    Jin Jiang

  4. Department of Anatomy & Cell Biology, University of Florida College of Medicine, Gainesville, Florida

    L. Shannon Holliday

  5. Department of Orthodontics, University of Florida College of Dentistry, 1600 SW Archer Road, Box 100444, Gainesville, Florida, 32610

    L. Shannon Holliday

Authors
  1. L. Shannon Holliday
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  2. Michael R. Bubb
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  3. Jin Jiang
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  4. I. Rita Hurst
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  5. Jian Zuo
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Correspondence to L. Shannon Holliday.

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Holliday, L.S., Bubb, M.R., Jiang, J. et al. Interactions Between Vacuolar H+-ATPases and Microfilaments in Osteoclasts. J Bioenerg Biomembr 37, 419–423 (2005). https://doi.org/10.1007/s10863-005-9483-y

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  • DOI: https://doi.org/10.1007/s10863-005-9483-y

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