Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 6, pp 419–423 | Cite as

Interactions Between Vacuolar H+-ATPases and Microfilaments in Osteoclasts

  • L. Shannon Holliday
  • Michael R. Bubb
  • Jin Jiang
  • I. Rita Hurst
  • Jian Zuo


Vacuolar H+-ATPases (V-ATPases) are transported from cytosolic compartments to the ruffled plasma membrane of osteoclasts as they activate to resorb bone. Transport of V-ATPases is essential for bone resorption, and is associated with binding interactions between V-ATPases and microfilaments that are mediated by an actin-binding site in subunit B. This site is contained within 44 amino acids in the amino terminal domain, and requires a sequence motif that resembles an actin-binding motif found in mammalian profilin 1. Small alterations in the profilin-like sequence disrupt the actin-binding activity of subunit B. The interaction between V-ATPases and microfilaments in osteoclasts is regulated in response to changes in phosphatidylinositol-3 kinase activity. During internalization of V-ATPases from the plasma membrane of osteoclasts after a cycle of resorption, V-ATPases bind microfilaments that are in podosomes, dynamic actin-based structures, also present in metastatic cancer cells. Studies are ongoing to establish the physiological role of the microfilament-binding activity of subunit B in osteoclasts and in other cells.

Key Words

V-ATPase actin bone ruffled membranes membrane trafficking phosphatidylinositol 3-kinase actin-related protein 2/3 complex PI 3-kinase cortactin 


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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • L. Shannon Holliday
    • 1
    • 4
    • 5
  • Michael R. Bubb
    • 2
  • Jin Jiang
    • 3
  • I. Rita Hurst
    • 1
  • Jian Zuo
    • 1
  1. 1.Department of OrthodonticsUniversity of Florida College of DentistryGainesville
  2. 2.Malcom Randall VA Medical CenterGainesville
  3. 3.University of Connecticut Health Science CenterFarmington
  4. 4.Department of Anatomy & Cell BiologyUniversity of Florida College of MedicineGainesville
  5. 5.Department of OrthodonticsUniversity of Florida College of DentistryGainesville

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