An Inception Report on the TOM Complex of the Amoeba Acanthamoeba castellanii, a Simple Model Protozoan in Mitochondria Studies
- 83 Downloads
It is suggested that in the course of the TOM complex evolution at least two lineages have appeared: the animal–fungal and green plant ones. The latter involves also the TOM complexes of algae and protozoans. The amoeba Acanthamoeba castellanii is a free-living nonphotosynthetic soil protozoan, whose mitochondria share many bioenergetic properties with mitochondria of plants, animals and fungi. Here, we report that a protein complex, identified electrophysiologically as the A. castellanii TOM complex, contains a homologue of yeast/animal Tom70. Further, molecular weight of the complex (about 500 kDa) also points to A. castellanii evolutionary relation with fungi and animal. Thus, the data indicates that the TOM complex of A. castellanii is not a typical example of the protozoan TOM complex.
KeywordsAcanthamoeba castellanii TOM complex Tom70
electrospray ionization tandem mass spectrometry
matrix-assisted laser desorption/ionization mass spectrometry
translocase of the outer membrane
voltage-dependent anion-selective channel
Unable to display preview. Download preview PDF.
- Douce, R., Bourguignon, R., and Neuberger, M. (1984). Methods Enzymol. 148, 403–415.Google Scholar
- Neff, R. J., and Neff, R. H. (1964). In Methods in Cell Physiology, (Prescott, D., ed.), Academic Press, New York, pp. 213–245.Google Scholar
- Sluse, F. E. and Jarmuszkiewicz, W. (2001). In Handbook of plant growth: pH as the master variable, (Rengel., Z., ed.), Marcel Dekker, Inc., New York, Basel, pp. 173–209.Google Scholar