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Journal of Bioenergetics and Biomembranes

, Volume 37, Issue 2, pp 97–107 | Cite as

In Phosphorylating Acanthamoeba castellanii Mitochondria the Sensitivity of Uncoupling Protein Activity to GTP Depends on the Redox State of Quinone

  • Wieslawa Jarmuszkiewicz
  • Aleksandra Swida
  • Malgorzata Czarna
  • Nina Antos
  • Claudine M. Sluse-Goffart
  • Francis E. Sluse
Article

Abstract

In isolated Acanthamoeba castellanii mitochondria respiring in state 3 with external NADH or succinate, the linoleic acid-induced purine nucleotide-sensitive uncoupling protein activity is able to uncouple oxidative phosphorylation. The linoleic acid-induced uncoupling can be inhibited by a purine nucleotide (GTP) when quinone (Q) is sufficiently oxidized, indicating that in A. castellanii mitochondria respiring in state 3, the sensitivity of uncoupling protein activity to GTP depends on the redox state of the membranous Q. Namely, the inhibition of the linoleic acid-induced uncoupling by GTP is not observed in uninhibited state 3 respiration as well as in state 3 respiration progressively inhibited by complex III inhibitors, i.e., when the rate of quinol (QH2)-oxidizing pathway is decreased. On the contrary, the progressive decrease of state 3 respiration by declining respiratory substrate availability (by succinate uptake limitation or by decreasing external NADH concentration), i.e., when the rate of Q-reducing pathways is decreased, progressively leads to a full inhibitory effect of GTP. Moreover, in A. castellanii mitochondria isolated from cold-treated cells, where a higher uncoupling protein activity is observed, the inhibition of the linoleic acid-induced proton leak by GTP is revealed for the same low values of the Q reduction level.

Keywords

Mitochondria uncoupling protein purine nucleotide inhibition quinone redox state Acanthamoeba castellanii 

Abbreviations:

ΔμH+

proton electrochemical gradient

UCP

uncoupling protein

UCP1

uncoupling protein of brown adipose tissue mitochondria

FFA

free fatty acids

ΔΨ

mitochondrial membrane potential

LA

linoleic acid

BSA

bovine serum albumin

BHAM

benzohydroxamate

AcUCP

uncoupling protein of Acanthamoeba castellanii mitochondria

Q

quinone

Qox

oxidized quinone

Qred or QH2

reduced quinone (quinol)

Qtot

total endogenous pool of quinone in the inner mitochondrial membrane (Qox + Qred)

Qred/Qtot

reduction level of quinone

Jo

respiratory rate in phosphorylating state 3

Jp

rate of ATP synthesis

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Wieslawa Jarmuszkiewicz
    • 1
    • 3
  • Aleksandra Swida
    • 1
  • Malgorzata Czarna
    • 1
  • Nina Antos
    • 1
  • Claudine M. Sluse-Goffart
    • 2
  • Francis E. Sluse
    • 2
  1. 1.Laboratory of BioenergeticsAdam Mickiewicz UniversityPoznanPoland
  2. 2.Laboratory of Bioenergetics, Department of Life Sciences and Centre of Oxygen Research and Development, Institute of Chemistry B6cUniversity of LiegeLiegeBelgium
  3. 3.Laboratory of BioenergeticsAdam Mickiewicz UniversityPoznanPoland

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