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Journal of Bioenergetics and Biomembranes

, Volume 36, Issue 6, pp 525–531 | Cite as

Regulation by Magnesium of Potato Tuber Mitochondrial Respiratory Activities

  • Joaquim A. F. Vicente
  • Vítor M. C. Madeira
  • Anibal E. Vercesi
Article

Abstract

Dehydrogenase activities of potato tuber mitochondria and corresponding phosphorylation rates were measured for the dependence on external and mitochondrial matrix Mg2+. Magnesium stimulated state 3 and state 4 respiration, with significantly different concentrations of matrix Mg2+ required for optimal activities of the several substrates. Maximal stimulation of respiration with all substrates was obtained at 2-mM external Mg2+. However, respiration of malate, citrate, and α-ketoglutarate requires at least 4-mM Mg2+ inside mitochondria for maximization of dehydrogenase activities. The phosphorylation system, requires a low level of internal Mg2+ (0.25 mM) to reach high activity, as judged by succinate-dependent respiration. However, mitochondria respiring on citrate or α-ketoglutarate only sustain high levels of phosphorylation with at least 4-mM matrix Mg2+. Respiration of succinate is active without external and matrix Mg2+, although stimulated by the cation. Respiration of α-ketoglutarate was strictly dependent on external Mg2+ required for substrate transport into mitochondria, and internal Mg2+ is required for dehydrogenase activity. Respiration of citrate and malate also depend on internal Mg2+ but, unlike α-ketoglutarate, some activity still remains without external Mg2+. All the substrates revealed insensitive to external and internal mitochondrial Ca2+, except the exogenous NADH dehydrogenase, which requires either external Ca2+ or Mg2+ for detectable activity. Calcium is more efficient than Mg2+, both having cumulative stimulation. Unlike Ca2+, Mn2+ could substitute for Mg2+, before and after addition of A23, showing its ability to regulate phosphorylation and succinate dehydrogenase activities, with almost the same efficiency as Mg2+.

Keywords

Magnesium regulation plant mitochondria mitochondrial respiration A23187 mitochondrial Mg2+ depletion 

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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Joaquim A. F. Vicente
    • 1
    • 4
  • Vítor M. C. Madeira
    • 2
  • Anibal E. Vercesi
    • 3
  1. 1.Departamento de BotânicaUniversidade de CoimbraCoimbraPortugal
  2. 2.Departamento de Bioquímica da Faculdade de Ciências e TecnologiaUniversidade de CoimbraCoimbraPortugal
  3. 3.Departamento de Patologia Clínica, Faculdade de Ciências MédicasUniversidade Estadual de CampinasCampinasBrasil
  4. 4.Departamento de Zoologia, FCTUniversidade de CoimbraCoimbraPortugal

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