Fig. 4 | Journal of Biomolecular NMR

Fig. 4

From: Efficient production of a functional G protein-coupled receptor in E. coli for structural studies

Fig. 4

Quality test of E. coli-derived β1AR by the CPM thermal shift assay. a Thermal stability of E. coli-derived full-length MBP-TS-β1AR-TrxA and TS-β1AR in comparison to insect-derived TS-β1AR (all apo forms). Samples contained ~ 25 μM receptor in 20 mM Tris, 1 mM EDTA, 750 mM NaCl, 0.1% DM, pH 7.5. b Thermal stability of E. coli-derived TS-β1AR in the presence of various additives. The CPM fluorescence signal (F) is shown as its first derivative (dF/dT). Triplicates of the experiments are shown color-coded by identical colors

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