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Automated projection spectroscopy in solid-state NMR

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Abstract

Given that solid-state NMR is being used for protein samples of increasing molecular weight and complexity, higher-dimensionality methods are likely to be more and more indispensable for unambiguous chemical shift assignments in the near future. In addition, solid-state NMR spectral properties are increasingly comparable with solution NMR, allowing adaptation of more sophisticated solution NMR strategies for the solid state in addition to the conventional methodology. Assessing first principles, here we demonstrate the application of automated projection spectroscopy for a micro-crystalline protein in the solid state.

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Acknowledgements

The authors acknowledge Karin Giller and Dr. Stefan Becker for sample preparation and Prof. Dr. Sebastian Hiller for APSY-related scripts. Financial support is acknowledged from the Deutsche Forschungsgemeinschaft (SFB 749, TP A13, SFB 1309, TP A03, as well as the Emmy Noether program), the Excellence Clusters CiPS-M and RESOLV, and the Center for NanoScience (CeNS).

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Authors and Affiliations

  1. Department of Chemistry, Ludwig-Maximilians-University Munich, Butenandtstr. 5-13, 81377, Munich, Germany

    Alexander Klein, Suresh K. Vasa & Rasmus Linser

  2. Center for Integrated Protein Science (CiPSM), Munich, Germany

    Alexander Klein, Suresh K. Vasa & Rasmus Linser

  3. Faculty of Chemistry and Chemical Biology, Technical University Dortmund, Otto-Hahn-Straße 4a, 44227, Dortmund, Germany

    Rasmus Linser

Authors
  1. Alexander Klein
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  2. Suresh K. Vasa
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  3. Rasmus Linser
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Correspondence to Rasmus Linser.

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Klein, A., Vasa, S.K. & Linser, R. Automated projection spectroscopy in solid-state NMR. J Biomol NMR 72, 163–170 (2018). https://doi.org/10.1007/s10858-018-0215-0

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  • DOI: https://doi.org/10.1007/s10858-018-0215-0

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