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Journal of Biomolecular NMR

, Volume 63, Issue 2, pp 223–228 | Cite as

Structure of the α-crystallin domain from the redox-sensitive chaperone, HSPB1

  • Ponni Rajagopal
  • Ying Liu
  • Lei Shi
  • Amanda F. Clouser
  • Rachel E. Klevit
NMR structure note

Biological context

Cells subject to various stresses such as ischemia, oxidation, and heat shock are at risk of accumulating unfolded or misfolded proteins that can aggregate and negatively affect cellular function. Chaperones are expressed at high levels under stress conditions to (1) maintain proteins in soluble states and (2) help proteins regain their native fold. Small Heat Shock Proteins (sHSPs) are ATP-independent chaperones that perform the former function and HSP90 or HSP70 are ATP-dependent chaperones that perform the latter. Ten sHSPs are encoded by the human genome. Progress towards structural and functional understanding of sHSPs has proven challenging. sHSPs form dynamic supramolecular and oligomeric structures from subunits that are ~20 kDa in size. Two pseudo-atomic structural models of HSPB5 24-mers composed of full-length subunits, (also known as αB-crystallin and CRYAB) have been determined using a combination of techniques including solid-state NMR, Small Angle...

Keywords

NOESY Spectrum Dime Interface Residual Dipolar Coupling Sedimentation Velocity Experiment Mammalian sHSPs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank David Baker for access to RosettaOligomer software and computing facilities, William Atkins for use of his fluorimeter, and Hannah Baughman for help with fluorescence experiments. The work was supported by NIH Grant 1R01 EY017370 (to REK).

Supplementary material

10858_2015_9973_MOESM1_ESM.pdf (388 kb)
Supplementary material 1 (PDF 387 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of WashingtonSeattleUSA

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