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Journal of Biomolecular NMR

, Volume 57, Issue 4, pp 319–326 | Cite as

SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR

  • Lucio Ferella
  • Claudio Luchinat
  • Enrico Ravera
  • Antonio Rosato
Communication

Abstract

We have proposed solid state NMR (SSNMR) of sedimented solutes as a novel approach to sample preparation for biomolecular SSNMR without crystallization or other sample manipulations. The biomolecules are confined by high gravity—obtained by centrifugal forces either directly in a SSNMR rotor or in a ultracentrifugal device—into a hydrated non-crystalline solid suitable for SSNMR investigations. When gravity is removed, the sample reverts to solution and can be treated as any solution NMR sample. We here describe a simple web tool to calculate the relevant parameters for the success of the experiment.

Keywords

Ultracentrifuge Sedimentation Solid state NMR Biological macromolecules Aggregates 

Notes

Acknowledgments

This work has been supported by the EC contracts East-NMR No. 228461, WeNMR No. 261572 and Bio-NMR No. 261863, INSTRUCT (European FP7 e-Infrastructure Grant, Contract No. 211252, http://www.instruct-fp7.eu/), the project PRIN (2009FAKHZT_001) “Biologia strutturale meccanicistica: avanzamenti metodologici e biologici” and Ente Cassa Risparmio Firenze. We thank Frank Engelke (Bruker Biospin), Paolo Santino (Agilent Tech.), Yusuke Nishiyama (Jeol) and F. David Doty (Doty Scientific) for providing rotor specifications.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Lucio Ferella
    • 1
  • Claudio Luchinat
    • 1
    • 2
    • 3
  • Enrico Ravera
    • 1
    • 3
  • Antonio Rosato
    • 1
    • 3
  1. 1.Center for Magnetic Resonance (CERM)University of FlorenceSesto FiorentinoItaly
  2. 2.Fondazione Farmacogenomica FiorGen OnlusSesto FiorentinoItaly
  3. 3.Department of ChemistryUniversity of FlorenceSesto FiorentinoItaly

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