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Journal of Biomolecular NMR

, Volume 56, Issue 4, pp 365–377 | Cite as

PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer

  • Vipin Agarwal
  • Mariana Sardo
  • Ingo Scholz
  • Anja Böckmann
  • Matthias Ernst
  • Beat H. Meier
Article

Abstract

In this article, we describe third-spin assisted heteronuclear recoupling experiments, which play an increasingly important role in measuring long-range heteronuclear couplings, in particular 15N–13C, in proteins. In the proton-assisted insensitive nuclei cross polarization (PAIN-CP) experiment (de Paëpe et al. in J Chem Phys 134:095101, 2011), heteronuclear polarization transfer is always accompanied by homonuclear transfer of the proton-assisted recoupling (PAR) type. We present a phase-alternating experiment that promotes heteronuclear (e.g. 15N → 13C) polarization transfer while simultaneously minimizing homonuclear (e.g.13C → 13C) transfer (PAIN without PAR). This minimization of homonuclear polarization transfer is based on the principle of the resonant second-order transfer (RESORT) recoupling scheme where the passive proton spins are irradiated by a phase-alternating sequence and the modulation frequency is matched to an integer multiple of the spinning frequency. The similarities and differences between the PAIN-CP and this het-RESORT experiment are discussed here.

Keywords

Solid-state NMR Heteronuclear correlation PAIN-CP RESORT Heteronuclear RESORT 

Notes

Acknowledgments

Financial support was provided by the Swiss National Science Foundation (Grant 200020_124611), the ETH Zurich and the CNRS (ANR-12-BS08-0013-01 XLproteinSSNMR). M.S. also acknowledges the Portuguese Foundation for Science and Technology for a post-doctoral grant—SFRH/BPD/65978/2009. We also acknowledge support from the European Commission under the Seventh Framework Programme (FP7), contract Bio-NMR 261863.

Supplementary material

10858_2013_9756_MOESM1_ESM.pdf (816 kb)
Supplementary material 1 (PDF 815 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Physical ChemistryETH ZurichZurichSwitzerland
  2. 2.Chemistry Department, CICECOUniversity of AveiroAveiroPortugal
  3. 3.IBCPUMR 5086 CNRS, Université de Lyon 1LyonFrance

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