Journal of Biomolecular NMR

, Volume 54, Issue 1, pp 115–121 | Cite as

NMR structure note: solution structure of Ca2+ binding domain 2B of the third isoform of the Na+/Ca2+ exchanger

  • Vincent Breukels
  • Wouter G. Touw
  • Geerten W. Vuister
NMR structure note


Resonance Assignment Isothermal Titration Calorimetry Backbone Dynamic Alignment Medium Side Chain Chemical Shift 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Ca2+ binding domain 2


4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid


Macroscopic dissociation constant


Isothermal titration calorimetry


Na+/Ca2+ exchanger isoform 3


Nuclear Overhauser effect


Optical density at 600 nm


Residual dipolar coupling


Root mean square


Root mean square deviation


Sodium dodecyl sulfate polyacrylamide gel electrophoresis



We thank Jurgen Doreleijers for assistance with Xplor-NIH and iCing, Gjalt van Rutten for support with using the HPC Cloud infrastructure at BitBrains. This work was supported by grants from the Netherlands Organization for Scientific Research (NWO): 700.55.443 and 700.57.101.

Supplementary material

10858_2012_9654_MOESM1_ESM.pdf (3.2 mb)
Supplementary material 1 (PDF 3261 kb)


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Vincent Breukels
    • 1
    • 2
  • Wouter G. Touw
    • 1
  • Geerten W. Vuister
    • 3
  1. 1.Institute for Molecules and MaterialsRadboud University NijmegenNijmegenThe Netherlands
  2. 2.Department of RadiologyRadboud University Nijmegen Medical CentreNijmegenThe Netherlands
  3. 3.Department of BiochemistryUniversity of LeicesterLeicesterUK

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