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NMR structure note: solution structure of Ca2+ binding domain 2B of the third isoform of the Na+/Ca2+ exchanger

  • NMR structure note
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Fig. 1
Fig. 2

Abbreviations

CBD2:

Ca2+ binding domain 2

HEPES:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

KD :

Macroscopic dissociation constant

ITC:

Isothermal titration calorimetry

NCX3:

Na+/Ca2+ exchanger isoform 3

NOE:

Nuclear Overhauser effect

OD600 :

Optical density at 600 nm

RDC:

Residual dipolar coupling

r.m.s.:

Root mean square

r.m.s.d.:

Root mean square deviation

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Acknowledgments

We thank Jurgen Doreleijers for assistance with Xplor-NIH and iCing, Gjalt van Rutten for support with using the HPC Cloud infrastructure at BitBrains. This work was supported by grants from the Netherlands Organization for Scientific Research (NWO): 700.55.443 and 700.57.101.

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Author notes

  1. Vincent Breukels

    Present address: Department of Radiology, Radboud University Nijmegen Medical Centre, Geert Grooteplein 10, 6526 GA, Nijmegen, The Netherlands

Authors and Affiliations

  1. Institute for Molecules and Materials, Radboud University Nijmegen, Geert Grooteplein 26-28, 6525 GA, Nijmegen, The Netherlands

    Vincent Breukels & Wouter G. Touw

  2. Department of Biochemistry, University of Leicester, Henry Wellcome Building, Lancaster Road, Leicester, LE1 9HN, UK

    Geerten W. Vuister

Authors
  1. Vincent Breukels
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  2. Wouter G. Touw
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Correspondence to Geerten W. Vuister.

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Breukels, V., Touw, W.G. & Vuister, G.W. NMR structure note: solution structure of Ca2+ binding domain 2B of the third isoform of the Na+/Ca2+ exchanger. J Biomol NMR 54, 115–121 (2012). https://doi.org/10.1007/s10858-012-9654-1

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