Abstract
In this study we investigated the dynamic behavior of the chimeric cell-penetrating peptide transportan in membrane-like environments using NMR. Backbone amide 15N spin relaxation was used to investigate the dynamics in two bicelles: neutral DMPC bicelles and partly negatively charged DMPG-containing bicelles. The structure of the peptide as judged from CD and chemical shifts is similar in the two cases. Both the overall motion as well as the local dynamics is, however, different in the two types of bicelles. The overall dynamics of the peptide is significantly slower in the partly negatively charged bicelle environment, as evidenced by longer global correlation times for all measured sites. The local motion, as judged from generalized order parameters, is for all sites in the peptide more restricted when bound to negatively charged bicelles than when bound to neutral bicelles (increase in S 2 is on average 0.11 ± 0.07). The slower dynamics of transportan in charged membrane model systems cause significant line broadening in the proton NMR spectrum, which in certain cases limits the observation of 1H signals for transportan when bound to the membrane. The effect of transportan on DMPC and DHPC motion in zwitterionic bicelles was also investigated, and the motion of both components in the bicelle was found to be affected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CPMG:
-
Carr-Purcell-Meiboom-Gill
- CPP:
-
cell-penetrating peptide
- DHPC:
-
1,2-dihexanoyl-sn-glycero-3-phosphocholine
- DHPC-d 22 :
-
deuterated 1,2-dihexanoyl-sn-glycero-3-phosphocholine
- DMPC:
-
1,2-dimyristoyl-sn-glycero-3-phosphocholine
- DMPC-d 54 :
-
deuterated 1,2-dimyristoyl-sn-glycero-3-phosphocholine
- DMPG-d 54 :
-
deuterated 1,2-dimyrisotoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
- HSQC:
-
heteronuclear single quantum coherence
- NMR:
-
nuclear magnetic resonance
- NOE:
-
nuclear Overhauser enhancement
- PNAs:
-
peptide nucleic acids
- siRNA:
-
small interfering RNA
References
Andersson A., Almqvist J., Hagn F., Mäler L. (2004) Biochim. Biophys. Acta. 1661:18–25
Andersson A., Mäler L. (2003) FEBS Lett. 545:139–143
Andersson, A. and Mäler, L. (2005) Langmuir. 21:7702–7709
Bárány-Wallje E., Andersson A., Gräslund A., Mäler L. (2004) FEBS Lett. 567:265–269
Biverståhl H., Andersson A., Gräslund A., Mäler L. (2004) Biochemistry. 43:14940–14947
Callaghan P., Komlosh M., Nydén M. (1998) J. Magn. Reson. 133:177–182
Carr H.Y., Purcell E.M. (1954) Phys. Rev. 94:630–638
Damberg P., Jarvet J., Gräslund A. (2001) J. Magn. Reson. 148:343–348
Derossi D., Chassaing G., Prochiantz A. (1998) Trends Cell Biol. 8:84–87
Deshayes S., Morris M.C., Divita G., Heitz F. (2005) Cell. Mol. Life Sci. 62:1839–1849
Drin G., Cottin S., Blanc E., Rees A.R., Temsamani J. (2003) J. Biol. Chem. 278:31192–31201
Glover K.J., Whiles J.A., Wu G., Yu N.-J., Deems R., Struppe J.O., Stark R.E., Komives E.A., Vold R.R. (2001) Biophys. J. 81:2163–2171
Greenfield N, Fasman G.D. (1969) Biochemistry. 8:4108–4116
Järver P, Langel Ü. (2004) Drug Discov. Today. 9:395–402
Jones S.W., Christison R., Bundell K., Voyce C.J., Brockbank S.M.V., Newham P., Lindsay M.A. (2005) Br. J. Pharmacol. 145:1093–1102
Kay L.E., Keifer P., Saarinen T. (1992) J. Am. Chem. Soc. 114:10663–10665
Lakowicz J.R. (1999) Principles of Fluorescence Spectroscopy 2nd edn. Kluwer Academic, New York
Lee L.K., Rance M., Chazin W.J., Palmer III A.G. (1997) J. Biomol. NMR. 9:287–298
Lipari G., Szabo A. (1982a) J. Am. Chem. Soc. 104:4546–4559
Lipari G., Szabo A. (1982b) J. Am. Chem. Soc. 104:4559–4570
Lundberg M., Johansson M. (2002) Biochem. Biophys. Res. Commun. 291:367–371
Magzoub M., Gräslund A. (2004) Q. Rev. Biophys. 37:147–195
Magzoub M., Kilk K., Eriksson L.E.G., Langel Ü., Gräslund A. (2001) Biochim. Biophys. Acta 1516:77–89
Mandel A.M., Akke M., Palmer III A.G. (1995) J. Mol. Biol. 246:144–163
Meiboom S., Gill D. (1958) Rev. Sci. Instrum. 29:688–691
Palmer III A.G., Kroenke C.D., Loria P.J. (2001) Meth. Enzymol. 339:204–238
Palmer III A.G., Rance M., Wright P. (1991) J. Am. Chem. Soc. 113:4371–4380
Papadopoulos, E., Oglecka, K., Mäler, L., Jarvet, J., Wright, P.E., Dyson, J. and Gräslund, A. (2006) Biochemistry 45, 159–166
Pooga M., Hällbrink M., Zorko M., Langel Ü. (1998) FASEB J. 12:67–77
Richard J.P., Melikov K., Vives E., Ramos C., Verbeure B., Gait M.J., Chernomordik L.V., Lebleu B. (2003) J. Biol. Chem. 278:585–590
Sakai N., Matile S. (2003) J. Am. Chem. Soc. 125:14348–14356
Skelton N.J., Palmer III A.G., Akke M., Kördel J., Rance M., Chazin W.J. (1993) J. Magn. Reson. B. 102:253–264
Stejskal E.O., Tanner J.E. (1965) J. Chem. Phys. 42:288–292
Struppe J., Whiles J.A., Vold R.R. (2000) Biophys. J. 78:281–289
Terrone D., Sang S.L.W., Roudaia L., Silvius J.R. (2003) Biochemistry. 42:13787–13799
Vold R.R., Prosser R.S. (1996) J. Magn. Reson. B. 113:267–271
Vold R.R., Prosser S.R., Deese A.J. (1997) J. Biomol. NMR. 9:329–335
Wang C., Grey M.J., Palmer III A.G. (2001) J. Biomol. NMR. 21:361–366
Acknowledgements
We wish to thank Joshua Hicks for helpful insights and careful reading of the manuscript. This work is supported by grants from the Swedish Research Council and the Carl Trygger Foundation.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Bárány-Wallje, E., Andersson, A., Gräslund, A. et al. Dynamics of transportan in bicelles is surface charge dependent. J Biomol NMR 35, 137–147 (2006). https://doi.org/10.1007/s10858-006-9008-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10858-006-9008-y