Advertisement

Journal of Biomolecular NMR

, Volume 33, Issue 1, pp 69–72 | Cite as

Solution Structure of Human Dihydrofolate Reductase in its Complex with Trimethoprim and NADPH

  • Nadezhda V. Kovalevskaya
  • Yegor D. Smurnyy
  • Vladimir I. Polshakov
  • Berry Birdsall
  • Alan F. Bradbury
  • Tom Frenkiel
  • James Feeney
NMR structure note

Key words

co-operative ligand binding dihydrofolate reductase protein structure protein–ligand interactions trimethoprim 

Abbreviations

DHFR

dihydrofolate reductase

hDHFR

human dihydrofolate reductase

MTX

methotrexate

PDB

Protein Data Bank

TMP

trimethoprim

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

supp.doc (60 kb)
Supplementary material
SuppImage_hi_res1.tif (9.8 mb)
Supplementary image (high resolution)
SuppImage_hi_res2.tif (50.6 mb)
Supplementary image (high resolution)
SuppImage_hi_res3.tif (20.4 mb)
Supplementary image (high resolution)
SuppImage_hi_res4.tif (10.9 mb)
Supplementary image (high resolution)
SuppImage_hi_res5.tif (5.5 mb)
Supplementary image (high resolution)
SuppImage_hi_res6.tif (42.9 mb)
Supplementary image (high resolution)
SuppImage_hi_res7.tif (3.2 mb)
Supplementary image (high resolution)

References

  1. Baccanari, D.P., Kuyper, L.F. 1993J. Chemother.5393399PubMedGoogle Scholar
  2. Bartels, Ch., Xia, T.-H., Billeter, M., Güntert, P., Wüthrich, K. 1995J. Biomol. NMR5110CrossRefPubMedGoogle Scholar
  3. Birdsall, B., Andrews, J., Ostler, G., Tendler, S.J.B., Feeney, J., Roberts, G.C.K., Davies, R.W., Cheung, H.T.A. 1989Biochemistry2813531362CrossRefPubMedGoogle Scholar
  4. Brünger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T., Warren, G.L. 1998Acta Crystallogr. D Biol. Crystallogr.54905921CrossRefPubMedGoogle Scholar
  5. Cody, V., Luft, J.R., Pangborn, W., Gangjee, A. 2003Acta Crystallogr., Sect. D5916031609Google Scholar
  6. Cornilescu, G., Delaglio, F., Bax, A. 1999J. Biomol. NMR13289302CrossRefPubMedGoogle Scholar
  7. Coulson, C.J. 1995Molecular Mechanisms of Drug ActionTaylor & FrancisLondonGoogle Scholar
  8. Davies, J.F., Delcamp, T.J., Prendergast, N.J., Ashford, V.A., Freisheim, J.H., Kraut, J. 1990Biochemistry2994679479CrossRefPubMedGoogle Scholar
  9. Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A. 1995J. Biomol. NMR6277293CrossRefPubMedGoogle Scholar
  10. Habeck, M., Rieping, W., Linge, J.P., Nilges, M. 2004Methods Mol. Biol.278379402PubMedGoogle Scholar
  11. Hitchings, G.H. 1989Angew. Chem. Int. Ed.28879885CrossRefGoogle Scholar
  12. Johnson, J.M., Meiering, E.M., Wright, J.E., Pardo, J., Rosowsky, A., Wagner, G. 1997Biochemistry3643994411CrossRefPubMedGoogle Scholar
  13. Klon, A.E., Heroux, A., Ross, L.J., Pathak, V., Johnson, C.A., Piper, J.R., Borhani, D.W. 2002J. Mol. Biol.320677693CrossRefPubMedGoogle Scholar
  14. Laskowski, R.A., Rullmann, J.A.C., MacArthur, M.W., Kaptein, R., Thornton, J.M. 1996J. Biomol. NMR8477486CrossRefPubMedGoogle Scholar
  15. Polshakov, V.I., Frenkiel, T.A., Birdsall, B., Soteriou, A., Feeney, J. 1995J. Magn. Reson. B.1083143CrossRefPubMedGoogle Scholar
  16. Polshakov, V.I., Birdsall, B., Feeney, J. 1999Biochemistry381596215969CrossRefPubMedGoogle Scholar
  17. Polshakov, V.I., Smirnov, E.G., Birdsall, B., Kelly, G., Feeney, J. 2002J. Biomol. NMR246770CrossRefPubMedGoogle Scholar
  18. Prendergast, N.J., Delcamp, T.J., Smith, P.L., Freisheim, J.H. 1988Biochemistry2736643671CrossRefPubMedGoogle Scholar
  19. Roberts, G.C.K., Feeney, J., Burgen, A.S.V., Daluge, S. 1981FEBS Lett.1318588CrossRefPubMedGoogle Scholar
  20. Stockman, B.J., Nirmala, N.R., Wagner, G., Delcamp, T.J., DeYarman, M.T., Freisheim, J.H. 1992Biochemistry31218229CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Nadezhda V. Kovalevskaya
    • 1
  • Yegor D. Smurnyy
    • 2
  • Vladimir I. Polshakov
    • 1
    • 2
  • Berry Birdsall
    • 3
  • Alan F. Bradbury
    • 3
  • Tom Frenkiel
    • 4
  • James Feeney
    • 3
  1. 1.Center for Drug ChemistryMoscowRussia
  2. 2.Center for Magnetic Tomography and SpectroscopyM.V. Lomonosov Moscow State UniversityMoscowRussia
  3. 3.Division of Molecular StructureNational Institute for Medical ResearchLondonU.K.
  4. 4.MRC Biomedical NMR CentreNational Institute for Medical ResearchLondonU.K.

Personalised recommendations