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Characterization of doxycycline-loaded calcium phosphate cement: implications for treatment of aneurysmal bone cysts

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

Percutaneous doxycycline for treatment for aneurysmal bone cysts (ABCs) has been shown to decrease recurrence rates, however, this requires multiple procedures, includes the risks soft tissue necrosis, and does not provide structural support. We propose utilizing curettage with doxycycline-loaded calcium phosphate cement. This study aimed to evaluate the elution profile of doxycycline from calcium phosphate cement. Calcium phosphate cement underwent an in vitro elution protocol evaluating doxycycline concentrations of 0, 5, 10, and 15 mg/mL. Eluted concentrations were quantified utilizing high performance liquid chromatography at predetermined time points over 96 h. Compressive strength was evaluated both pre- and post-elution and micro-computed tomography was utilized to assess changes in cement porosity. Cement with 15 mg/mL of doxycycline maintained a higher average concentration (mean, 95% confidence intervals) (14.5 µg/mL [9.2–19.9 µg/mL]) compared to both 5 mg/mL (5.8 µg/mL [3.1–8.6 µg/mL]; P < 0.001) and 10 mg/mL (8.4 ± µg/mL [6.0–10.9 µg/mL]; P < 0.001). Ultimate stress significantly decreased between pre- and post-elution samples for 10 mg/mL (P= 0.001) and 15 mg/mL (P = 0.004) groups. This study demonstrated a dose-dependent response in ultimate strength and compressive modulus with addition of doxycycline to calcium phosphate cement.

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Acknowledgements

Authors would like to acknowledge Stryker (Mahwah, NJ, USA) for providing calcium phosphate cement for the completion of this study. This entity was not involved in study design, testing, data collection or analysis, or preparation of this manuscript.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Beaumont Health, 3601 W. 13 Mile Rd, Royal Oak, MI, 48073, USA

    Erika Daley, Michael D. Kurdziel, Denise Koueiter & Drew D. Moore

  2. Department of Orthopaedic Surgery, Oakland University-William Beaumont School of Medicine, 2200 N Squirrel Rd, Rochester, MI, 48309, USA

    Michael D. Kurdziel & Drew D. Moore

Authors
  1. Erika Daley
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  2. Michael D. Kurdziel
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  3. Denise Koueiter
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Correspondence to Drew D. Moore.

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Daley, E., Kurdziel, M.D., Koueiter, D. et al. Characterization of doxycycline-loaded calcium phosphate cement: implications for treatment of aneurysmal bone cysts. J Mater Sci: Mater Med 29, 109 (2018). https://doi.org/10.1007/s10856-018-6117-6

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  • DOI: https://doi.org/10.1007/s10856-018-6117-6

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