Silk fibroin-based woven endovascular prosthesis with heparin surface modification

  • Zekun Liu
  • Gang Li
  • Zhaozhu Zheng
  • Yuling Li
  • Yifan Han
  • David L. Kaplan
  • Xiaoqin Wang
Clinical Applications of Biomaterials Original Research
Part of the following topical collections:
  1. Clinical Applications of Biomaterials


A novel seamless silk fibroin-based endovascular prosthesis (SFEPs) with bifurcated woven structure and anticoagulant function for the improvement of patency is described. The SFEPs were prepared from silk fibroin (SF) and polyester filaments using an installed weaving machine. The production processing parameters were optimized using orthogonal design methods. The inner surface of SFEPs was modified with polyethylenimine (PEI) and EDC/NHS-activated low-molecular-weight heparin (LMWH) to enhance anticoagulant function. The surface morphology and mechanical properties of the SFEPs were evaluated according to standard protocols. The thickness of modified SFEPs was lower than 0.085 ± 0.004 mm and water permeability was lower than 5.19 ± 0.30 mL/(cm2 × min). The results of mechanical properties showed that the diametral tensile strength and burst strength reached 61.6 ± 1.8 and 23.7 ± 2.2 MPa, respectively. Automatic coagulometer and energy-dispersive X-ray (EDX) confirmed LMWH immobilization on the surface of the SFEPs and the blood compatibility was improved with the heparin modification with PEI polymerization. In conclusion, the new prosthesis has potential applications in the blood vessel repairs where minimal thickness but superior mechanical strength and biocompatibility are important.



This work was supported by the Natural Science Foundation of China (51603140), Natural Science Foundation of Jiangsu Province (BK20150372) and University Science Research Project of Jiangsu Province (16KJB540003). We would like to thank the support of China Postdoctoral Science Foundation, Municipal Science and Technology Project of Nantong and Key Industry Technology Innovation, Science and Technology Project of Suzhou (SYG201638) and Sino-Germany Joint Project (GZ1094).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Chlupáč J, Filova E, Bačáková L. Blood vessel replacement: 50 years of development and tissue engineering paradigms in vascular surgery. Physiol Res. 2009;58:S119–39.Google Scholar
  2. 2.
    Dong HK, Heo SJ, Yun GK, Ji WS, Park SH, Shin JW. Shear stress and circumferential stretch by pulsatile flow direct vascular endothelial lineage commitment of mesenchymal stem cells in engineered blood vessels. J Mater Sci Mater Med. 2016;27:1–10.CrossRefGoogle Scholar
  3. 3.
    Zhao H, Wang L, Li Y, Liu X, King MW. The mathematical model for evaluating fatigue resistance of SG tubular fabric: relationship between textile parameters and fatigue performance. J Biomater Appl. 2010;24:579–90.CrossRefGoogle Scholar
  4. 4.
    Boden M, Richard R, Schwarz MC, Kangas S, Huibregtse B, Barry JJ. In vitro and in vivo evaluation of the safety and stability of the TAXUS® Paclitaxel-Eluting Coronary Stent. J Mater Sci Mater Med. 2009;20:1553–62.CrossRefGoogle Scholar
  5. 5.
    Abdessalem SB, Durand B, Akesbi S, Chakfe N. Blood flow in a polyester textile vascular prosthesis: experimental and numerical study. Text Res J. 2001;71:178–83.CrossRefGoogle Scholar
  6. 6.
    Ma Z, Kotaki M, Yong T, He W, Ramakrishna S. Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering. Biomaterials. 2005;26:2527–36.CrossRefGoogle Scholar
  7. 7.
    Lovett M, Cannizzaro C, Daheron L, Messmer B, Vunjaknovakovic G, Kaplan DL. Silk fibroin microtubes for blood vessel engineering. Biomaterials. 2007;28:5271–79.CrossRefGoogle Scholar
  8. 8.
    Li G, Li Y, Chen G, He J, He Y, Wang X, Kaplan DL. Silk-based biomaterials in biomedical textiles and fiber-based implants. Adv Healthc Mater. 2015;4:1134–51.CrossRefGoogle Scholar
  9. 9.
    Guan Y, Yang X, Wang L, Guan G, King MW. A novel silk/polyester woven small diameter arterial prosthesis: degumming and the influence on cytocompatibility. Fibers Polym. 2015;16:1533–39.CrossRefGoogle Scholar
  10. 10.
    Enomoto S, Sumi M, Kan K, Nakazawa Y, Rui T, Takabayashi C, Asakura T, Sata M. Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material. J Cardiovasc Surg. 2010;51:155–64.Google Scholar
  11. 11.
    Podsiadlo P, Qin M, Cuddihy M, Zhu J, Critchley K, Kheng E, Kaushik AK, Qi Y, Kim H-S, Noh S-T, Arruda EM, Waas AM, Kotov NA. Highly ductile multilayered films by layer-by-layer assembly of oppositely charged polyurethanes for biomedical applications. Langmuir. 2009;25:14093–99.CrossRefGoogle Scholar
  12. 12.
    Vepari C, Matheson D, Drummy L, Naik R, Kaplan DL. Surface modification of silk fibroin with poly(ethylene glycol) for antiadhesion and antithrombotic applications. J Biomed Mater Res Part A. 2010;93:595–606.Google Scholar
  13. 13.
    Cui J, Koeverden MPV, Müllner M, Kempe K, Caruso F. Emerging methods for the fabrication of polymer capsules. Adv Colloid Interface Sci. 2014;207:14–31.CrossRefGoogle Scholar
  14. 14.
    Elahi MF, Guan G, Wang L, Zhao X, Wang F, King MW. Surface modification of silk fibroin fabric using layer-by-layer polyelectrolyte deposition and heparin immobilization for small diameter vascular prostheses. Langmuir. 2015;31:2517–26.CrossRefGoogle Scholar
  15. 15.
    Elahi M, Guan G, Wang L, King M. Influence of layer-by-layer polyelectrolyte deposition and EDC/NHS activated heparin immobilization onto silk fibroin fabric. Materials. 2014;7:2956–77.CrossRefGoogle Scholar
  16. 16.
    Dashwood MR, Anand R, Loesch A, Souza DS. Hypothesis: a potential role for the vasa vasorum in the maintenance of vein graft patency. Angiology. 2004;55:385–95.CrossRefGoogle Scholar
  17. 17.
    Bicknell CD, Cheshire NJ, Riga CV, Bourke P, Wolfe JH, Gibbs RG, Jenkins MP, Hamady M. Treatment of complex aneurysmal disease with fenestrated and branched stent grafts. Eur J Vasc Endovasc. 2009;37:175–81.CrossRefGoogle Scholar
  18. 18.
    Singh S, Wu BM, Dunn JCY. The enhancement of VEGF-mediated angiogenesis by polycaprolactone scaffolds with surface cross-linked heparin. Biomaterials. 2011;32:2059–69.CrossRefGoogle Scholar
  19. 19.
    Hu X, Kaplan DL. Peggy Cebe. Determining beta-sheet crystallinity in fibrous proteins by thermal analysis and infrared spectroscopy. Macromolecules. 2006;39:6161–70.CrossRefGoogle Scholar
  20. 20.
    Guidoin R, King M, Marceau D, Cardou A, De la Faye D, Legendre JM, Blais P. Textile arterial prostheses: Is water permeability equivalent to porosity? J Biomed Mater Res. 1987;21:65–87.CrossRefGoogle Scholar
  21. 21.
    Ji X, Wang L, King MW, Robert G. Physical characteristics of knitted polyester vascular prostheses: can the wall be considered as a scaffold for tissue engineering. J Donghua Uni. 2010;27:6–13.Google Scholar
  22. 22.
    Zhao J, Jing Z, Wang Z, Ye H, Bao J. Value of digital subtraction angiography in endovascular graft exclusion for abdominal aortic aneurysms. J Med Coll Pla. 2000;17:13–6.Google Scholar
  23. 23.
    Li G, Liu Y, Lan P, Li Y. A prospective bifurcated biomedical stent with seamless woven structure. J Text Inst. 2013;104:1017–23.CrossRefGoogle Scholar
  24. 24.
    Cestari M, Muller V, Rodrigues JH, Nakamura CV, Rubira AF, Muniz EC. Preparing silk fibroin nanofibers through electrospinning: further heparin immobilization toward hemocompatibility improvement. Biomacromolecules. 2014;15:1762–7.CrossRefGoogle Scholar
  25. 25.
    Zhang Y, Zhang C, Liu L, Kaplan DL, Zhu H, Lu Q. Hierarchical charge distribution controls self-assembly process of silk in vitro. Fron Mater Sci. 2015;9:382–91.CrossRefGoogle Scholar
  26. 26.
    Zhang G, Sheng M, Wei Z, Du Q, Chou LL. Self-assembly of silanated poly(ethylene glycol) on silicon and glass surfaces for improved haemocompatibility. Appl Surf Sci. 2009;255:6771–80.CrossRefGoogle Scholar
  27. 27.
    Wang Y, Li Y, Chen X, Yuan T. The design, manufacturing and performance analysis of ultrathin textile for endovascular exclusion vascular prosthesis. Tec Text. 2010;233:10–6.Google Scholar
  28. 28.
    Rousseau H, Puel J, Joffre F, Sigwart U, Duboucher C, Imbert C, Knight C, Kropf L, Wallsten H. Self-expanding endovascular prosthesis: an experimental study. J Cardiovasc Surg. 1987;8:709–14.Google Scholar
  29. 29.
    Wang H, Feng Y, Zhao H, Xiao R, Lu J, Li Z, Jing T. Electrospun hemocompatible PU/gelatin-heparin nanofibrous bilayer scaffolds as potential artificial blood vessels. Macromol Res. 2012;20:347–50.CrossRefGoogle Scholar
  30. 30.
    Yu J, Wang A, Tang Z, Henry J, Lee LP, Zhu Y, Yuan F, Huang F, Li S. The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration. Biomaterials. 2012;33:8062–74.CrossRefGoogle Scholar
  31. 31.
    Zhou M, Liu Z, Wei Z, Liu C, Qiao T, Ran F, Bai Y, Jiang X, Ding Y. Development and validation of small-diameter vascular tissue from a decellularized scaffold coated with heparin and vascular endothelial growth factor. Artif Organs. 2009;33:230–9.CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National Engineering Laboratory for Modern Silk, College of Textile and Clothing EngineeringSoochow UniversitySuzhouChina
  2. 2.College of TextilesDonghua UniversityShanghaiChina
  3. 3.Department of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHong KongChina
  4. 4.Department of Biomedical EngineeringTufts UniversityMedfordUSA

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