Silk fibroin-based woven endovascular prosthesis with heparin surface modification
A novel seamless silk fibroin-based endovascular prosthesis (SFEPs) with bifurcated woven structure and anticoagulant function for the improvement of patency is described. The SFEPs were prepared from silk fibroin (SF) and polyester filaments using an installed weaving machine. The production processing parameters were optimized using orthogonal design methods. The inner surface of SFEPs was modified with polyethylenimine (PEI) and EDC/NHS-activated low-molecular-weight heparin (LMWH) to enhance anticoagulant function. The surface morphology and mechanical properties of the SFEPs were evaluated according to standard protocols. The thickness of modified SFEPs was lower than 0.085 ± 0.004 mm and water permeability was lower than 5.19 ± 0.30 mL/(cm2 × min). The results of mechanical properties showed that the diametral tensile strength and burst strength reached 61.6 ± 1.8 and 23.7 ± 2.2 MPa, respectively. Automatic coagulometer and energy-dispersive X-ray (EDX) confirmed LMWH immobilization on the surface of the SFEPs and the blood compatibility was improved with the heparin modification with PEI polymerization. In conclusion, the new prosthesis has potential applications in the blood vessel repairs where minimal thickness but superior mechanical strength and biocompatibility are important.
This work was supported by the Natural Science Foundation of China (51603140), Natural Science Foundation of Jiangsu Province (BK20150372) and University Science Research Project of Jiangsu Province (16KJB540003). We would like to thank the support of China Postdoctoral Science Foundation, Municipal Science and Technology Project of Nantong and Key Industry Technology Innovation, Science and Technology Project of Suzhou (SYG201638) and Sino-Germany Joint Project (GZ1094).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Chlupáč J, Filova E, Bačáková L. Blood vessel replacement: 50 years of development and tissue engineering paradigms in vascular surgery. Physiol Res. 2009;58:S119–39.Google Scholar
- 10.Enomoto S, Sumi M, Kan K, Nakazawa Y, Rui T, Takabayashi C, Asakura T, Sata M. Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material. J Cardiovasc Surg. 2010;51:155–64.Google Scholar
- 11.Podsiadlo P, Qin M, Cuddihy M, Zhu J, Critchley K, Kheng E, Kaushik AK, Qi Y, Kim H-S, Noh S-T, Arruda EM, Waas AM, Kotov NA. Highly ductile multilayered films by layer-by-layer assembly of oppositely charged polyurethanes for biomedical applications. Langmuir. 2009;25:14093–99.CrossRefGoogle Scholar
- 12.Vepari C, Matheson D, Drummy L, Naik R, Kaplan DL. Surface modification of silk fibroin with poly(ethylene glycol) for antiadhesion and antithrombotic applications. J Biomed Mater Res Part A. 2010;93:595–606.Google Scholar
- 21.Ji X, Wang L, King MW, Robert G. Physical characteristics of knitted polyester vascular prostheses: can the wall be considered as a scaffold for tissue engineering. J Donghua Uni. 2010;27:6–13.Google Scholar
- 22.Zhao J, Jing Z, Wang Z, Ye H, Bao J. Value of digital subtraction angiography in endovascular graft exclusion for abdominal aortic aneurysms. J Med Coll Pla. 2000;17:13–6.Google Scholar
- 27.Wang Y, Li Y, Chen X, Yuan T. The design, manufacturing and performance analysis of ultrathin textile for endovascular exclusion vascular prosthesis. Tec Text. 2010;233:10–6.Google Scholar
- 28.Rousseau H, Puel J, Joffre F, Sigwart U, Duboucher C, Imbert C, Knight C, Kropf L, Wallsten H. Self-expanding endovascular prosthesis: an experimental study. J Cardiovasc Surg. 1987;8:709–14.Google Scholar
- 30.Yu J, Wang A, Tang Z, Henry J, Lee LP, Zhu Y, Yuan F, Huang F, Li S. The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration. Biomaterials. 2012;33:8062–74.CrossRefGoogle Scholar