Preparation of hydroxyapatite/collagen injectable bone paste with an anti-washout property utilizing sodium alginate. Part 1: influences of excess supplementation of calcium compounds
- 350 Downloads
The anti-washout property, viscosity, and cytocompatibility to an osteoblastic cell line, MG-63, of anti-washout pastes were investigated. Mixing a hydroxyapatite/collagen bone-like nanocomposite (HAp/Col), an aqueous solution of sodium alginate (Na-Alg), which is a paste hardening and lubricant agent, and supplementation of calcium carbonate or calcium citrate (Ca-Cit) as a calcium resource for the hardening reaction realized an injectable bone paste. Adding Ca-Cit at a concentration greater than eight times the Ca2+ ion concentration to Na-Alg improved the anti-washout property. Although the viscosity test indicated a gradual increase in the paste viscosity as the calcium compounds increased, pastes with excess supplementation of calcium compounds exhibited injectability through a syringe with a 1.8 mm inner diameter, realizing an injectable bone filler. Furthermore, the anti-washout pastes with Ca-Cit had almost the same cell proliferation rate as that of the HAp/Col dense body. Therefore, HAp/Col injectable anti-washout pastes composed of the HAp/Col, Na-Alg, and Ca-Cit are potential candidates for bioresorbable bone filler pastes.
KeywordsAlginate Dense Body Calcium Compound Washout Ratio Dicalcium Phosphate Dihydrate
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- 6.Konishi T, Mizumoto M, Honda M, Horiguchi Y, Oribe K, Morisue H, et al. Fabrication of novel biodegradable α-tricalcium phosphate cement set by chelating capability of inositol phosphate and its biocompatibility. J Nanomater. 2013; doi: 10.1155/2013/864374.
- 7.Konishi T, Takahashi S, Zhuang Z, Nagata K, Mizumoto M, Honda M, Takeuchi Y, Matsunari H, Nagashima H, Aizawa M. Biodegradable β-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate. J Mater Sci Mater Med. 2013;24:1383–94.CrossRefGoogle Scholar
- 12.Shinomiya K, Ishizuki M, Morioka H, Matasumoto S, Nakamura T, Abe S, Beppu Y. A phase III randomized controlled trial of selforganized hydroxyapatite/collagen composite versus beta tricalcium phosphate as bone substitute for treatment of osseous defect. Seikei Geka-Shi. 2012;63:921–6. [in Japanese].Google Scholar