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Evaluation of a laminin-alginate biomaterial, adipocytes, and adipocyte-derived stem cells interaction in animal autologous fat grafting model using 7-Tesla magnetic resonance imaging

  • Yo-Shen Chen
  • Yu-Sheng Hsueh
  • Yen-Yu Chen
  • Cheng-Yu Lo
  • Hao-Chih Tai
  • Feng-Huei Lin
Biomaterials Synthesis and Characterization Original Research
Part of the following topical collections:
  1. Biomaterials Synthesis and Characterization

Abstract

Biomaterials are often added to autologous fat grafts both as supporting matrices for the grafted adipocytes and as cell carrier for adipose-derived stem cells (ADSCs). This in vivo study used an autologous fat graft model to test a lamininalginate biomaterial, adipocytes, and ADSCs in immune-competent rats. We transplanted different combinations of shredded autologous adipose tissue [designated “A” for adipose tissue]), laminin-alginate beads [designated “B” for bead], and ADSCs [designated “C” for cell]) into the backs of 15 Sprague-Dawley rats. Group A received only adipocytes, Group B received only laminin-alginate beads, Group AB received adipocytes mixed with laminin-alginate beads, Group BC received laminin-alginate beads encapsulating ADSCs, and Group ABC received adipocytes and laminin-alginate beads containing ADSCs. Seven-tesla magnetic resonance imaging was used to evaluate the rats at the 1st, 6th, and 12th weeks after transplantation. At the 12th week, the rats were sacrificed and the implanted materials were retrieved for gross examination and histological evaluation. The results based on MRI, gross evaluation, and histological data all showed that implants in Group ABC had better resorption of the biomaterial, improved survival of the grafted adipocytes, and adipogenic differentiation of ADSCs. Volume retention of grafts in Group ABC (89%) was also significantly greater than those in Group A (58%) (p < 0.01). Our findings support that the combination of shredded adipose tissue with ADSCs in laminin-alginate beads provided the best overall outcome.

Keywords

Alginate Adipogenic Differentiation Volume Retention Mature Adipocyte Excellent Soft Tissue Contrast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Dr. Ya-Hui Tsai and Hsiao-Fang Wei for technical and statistic assistance. We also thank the 7T Animal MRI Core Lab of the Neurobiology and Cognitive Science Center, National Taiwan University for their technical support.

Funding

The experiments in this study were partially funded by Far Eastern Memorial Hospital (FEMH-2012-C-004). No other funding was received.

Author contributions

Y-S C: First author, partially financed the study and carried out the project. Y-SH: Provided instruction for creating the laminin-alginate beads. Y-YC: Original author of the methods for creating the laminin-alginate biomaterial. C-YL: Pathologist, provided support for histological staining and reading. H-CT: Adviser regarding the medical aspects of this study. F-HL: Professor at the Institute of Biomedical Engineering and project director.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yo-Shen Chen
    • 1
    • 2
  • Yu-Sheng Hsueh
    • 1
  • Yen-Yu Chen
    • 1
  • Cheng-Yu Lo
    • 3
  • Hao-Chih Tai
    • 4
  • Feng-Huei Lin
    • 1
    • 5
  1. 1.Institute of Biomedical EngineeringNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of Plastic SurgeryFar Eastern Memorial HospitalNew Taipei CityTaiwan
  3. 3.Department of PathologyFar Eastern Memorial HospitalNew Taipei CityTaiwan
  4. 4.Department of Plastic SurgeryNational Taiwan University HospitalTaipeiTaiwan
  5. 5.Institute of Biomedical Engineering and NanomedicineNational Health Research InstitutesMiaoliTaiwan

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