Evaluation of a laminin-alginate biomaterial, adipocytes, and adipocyte-derived stem cells interaction in animal autologous fat grafting model using 7-Tesla magnetic resonance imaging
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Biomaterials are often added to autologous fat grafts both as supporting matrices for the grafted adipocytes and as cell carrier for adipose-derived stem cells (ADSCs). This in vivo study used an autologous fat graft model to test a lamininalginate biomaterial, adipocytes, and ADSCs in immune-competent rats. We transplanted different combinations of shredded autologous adipose tissue [designated “A” for adipose tissue]), laminin-alginate beads [designated “B” for bead], and ADSCs [designated “C” for cell]) into the backs of 15 Sprague-Dawley rats. Group A received only adipocytes, Group B received only laminin-alginate beads, Group AB received adipocytes mixed with laminin-alginate beads, Group BC received laminin-alginate beads encapsulating ADSCs, and Group ABC received adipocytes and laminin-alginate beads containing ADSCs. Seven-tesla magnetic resonance imaging was used to evaluate the rats at the 1st, 6th, and 12th weeks after transplantation. At the 12th week, the rats were sacrificed and the implanted materials were retrieved for gross examination and histological evaluation. The results based on MRI, gross evaluation, and histological data all showed that implants in Group ABC had better resorption of the biomaterial, improved survival of the grafted adipocytes, and adipogenic differentiation of ADSCs. Volume retention of grafts in Group ABC (89%) was also significantly greater than those in Group A (58%) (p < 0.01). Our findings support that the combination of shredded adipose tissue with ADSCs in laminin-alginate beads provided the best overall outcome.
KeywordsAlginate Adipogenic Differentiation Volume Retention Mature Adipocyte Excellent Soft Tissue Contrast
We thank Dr. Ya-Hui Tsai and Hsiao-Fang Wei for technical and statistic assistance. We also thank the 7T Animal MRI Core Lab of the Neurobiology and Cognitive Science Center, National Taiwan University for their technical support.
The experiments in this study were partially funded by Far Eastern Memorial Hospital (FEMH-2012-C-004). No other funding was received.
Y-S C: First author, partially financed the study and carried out the project. Y-SH: Provided instruction for creating the laminin-alginate beads. Y-YC: Original author of the methods for creating the laminin-alginate biomaterial. C-YL: Pathologist, provided support for histological staining and reading. H-CT: Adviser regarding the medical aspects of this study. F-HL: Professor at the Institute of Biomedical Engineering and project director.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- 1.Coleman SR. Structural fat grafts: the ideal filler? Clin Plast Surg. 2001;28:111–9.Google Scholar
- 12.Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, Redl H, Rubin JP, Yoshimura K, Gimble JM. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15(6):641–8.CrossRefGoogle Scholar
- 16.Toyserkani NM, Quaade ML, Sørensen JA. Cell-assisted lipotransfer: a systematic review of its efficacy. Aesthet Plast Surg. 2016;40(2):309–18.Google Scholar
- 17.Feisst V, Meidinger S, Locke MB. From bench to bedside: use of human adipose-derived stem cells. Stem Cells Cloning. 2015;8:149–62.Google Scholar
- 20.Catalán V, Gómez-Ambrosi J, Rodríguez A, Frühbeck G. Role of extracellular matrix remodelling in adipose tissue pathophysiology: relevance in the development of obesity. Histol Histopathol. 2012;27(12):1515–28.Google Scholar
- 27.Chen YS, Chen YY, Hsueh YS, Tai HC, Lin FH. Modifying alginate with early embryonic extracellular matrix, laminin and hyaluronic acid for adipose tissue engineering. J Biomed Mater Res A. 2015;104(3):669–77. doi: 10.1002/jbm.a.35606.