Skip to main content
SpringerLink
Log in

Influence of chitosan-chitin nanofiber composites on cytoskeleton structure and the proliferation of rat bone marrow stromal cells

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Chitosan scaffolds have gained much attention in various tissue engineering applications, but the effect of their microstructure on cell-material spatial interactions remains unclear. Our objective was to evaluate the effect of chitosan-based matrices doping with chitin nano-whiskers (CNW) on adhesion, spreading, cytoskeleton structure, and proliferation of rat bone marrow stromal cells (BMSCs). The behavior of BMSCs during culture on chitosan-CNW films was determined by the molecular mass, hydrophobicity, porosity, crosslinking degree, protonation degree and molecular structure of the composite chitosan-CNW films. The shape, spreading area, cytoskeleton structure, and proliferation of BMSCs on chitosan matrices with a crystalline structure and high porosity were similar to that observed for BMSCs cultured on polystyrene tissue culture plates. The amorphous polymer structure and high swelling led to a decrease in the spreading area and cell proliferation. Thus, we can control the behavior of cells in culture (adhesion, spreading, and proliferation) by changing the physico-chemical properties of the chitosan-CNW films.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Lanza RP, Langer R, Vacanti JP. Principles of tissue engineering. 3rd ed. Academic Press; San Diego, CA, USA. 2011.

  2. Conget PA, Minguell JJ. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol. 1999;181(1):67–73. doi:10.1002/(SICI)1097-4652(199910)181:1<67::AID-JCP7>3.0.CO;2-C.

    Article  Google Scholar 

  3. Gao J, Yao JQ, Caplan AI. Stem cells for tissue engineering of articular cartilage. Proc Inst Mech Eng H. 2007;221(5):441–50.

    Article  Google Scholar 

  4. Bianco P, Riminucci M, Gronthos S, Robey PG. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells. 2001;19(3):180–92. doi:10.1634/stemcells.19-3-180.

    Article  Google Scholar 

  5. Ciapetti G, Ambrosio L, Marletta G, Baldini N, Giunti A. Human bone marrow stromal cells: in vitro expansion and differentiation for bone engineering. Biomaterials. 2006;27(36):6150–60. doi:10.1016/j.biomaterials.2006.08.025.

    Article  Google Scholar 

  6. Panarin EF, et al. Matrices for cell culture of human skin cells based on natural polysaccharides chitin and chitosan. Kletochnaya Transplantologiya i Tkanevaya Inzheneriya (Cell Transplantology and Tissue Engineering). 2009;4(3):42–6.

    Google Scholar 

  7. Shoichet MS. Polymer scaffolds for biomaterials applications. Macromolecules. 2010;43(2):581–91. doi:10.1021/ma901530r.

    Article  Google Scholar 

  8. Suh JK, Matthew HW. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials. 2000;21(24):2589–98.

    Article  Google Scholar 

  9. Afanas’eva NV, et al. Molecular mobility of chitosan and its interaction with montmorillonite in composite films: dielectric spectroscopy and FTIR studies. Poly Sci Series A. 2013;55(12):738–48. doi:10.1134/S0965545X13120018.

    Article  Google Scholar 

  10. Khan A, et al. Mechanical and barrier properties of nanocrystalline cellulose reinforced chitosan based nanocomposite films. Carbohydr Polym. 2012;90(4):1601–8. doi:10.1016/j.carbpol.2012.07.037.

    Article  Google Scholar 

  11. Petrova VA, et al. Specific features of chitosan-montmorillonite interaction in an aqueous acid solution and properties of related composite films. Poly Sci Series A. 2012;54(3):224–30. doi:10.1134/S0965545X1203008X.

    Article  Google Scholar 

  12. Shchipunov YA, Silant’ev VE, Postnova IV. Self-organization in the chitosan-clay nanoparticles system regulated through polysaccharide macromolecule charging. 1. Hydrogels. Colloid J. 2012;74(5):627–35. doi:10.1134/S1061933X12050092.

    Article  Google Scholar 

  13. Fan Y, Saito T, Isogai A. Individual chitin nano-whiskers prepared from partially deacetylated α-chitin by fibril surface cationization. Carbohydr Polym. 2010;79(4):1046–51. doi:10.1016/j.carbpol.2009.10.044.

    Article  Google Scholar 

  14. Kiroshka VV, et al. Adhesion, growth, and proliferation of endothelial cells on biopolymer extracellular film matrices. Bull Exp Biol Med. 2014;158(1):153–8. doi:10.1007/s10517-014-2712-9.

    Article  Google Scholar 

  15. Zotkin MA, Vikhoreva GA, Kechek’yan AS. Thermal modification of chitosan films in the form of salts with various acids. Poly Sci Series B. 2004;46(1-2):39–42.

    Google Scholar 

  16. Nud’ga LA, et al. Chemical and structural transformations in chitosan films in the course of storage. Russ J Appl Chem. 2008;81(11):1992–6. doi:10.1134/S1070427208110244.

    Article  Google Scholar 

  17. Pogodina NV, et al. Conformational characteristics of chitosan molecules as demonstrated by diffusion-sedimentation analysis and viscometry. Poly Sci USSR. 1986;28(2):251–9. doi:10.1016/0032-3950(86)90076-6.

    Article  Google Scholar 

  18. Kim K, Dean D, Mikos AG, Fisher JP. Effect of initial cell seeding density on early osteogenic signal expression of rat bone marrow stromal cells cultured on cross-linked poly(propylene fumarate) disks. Biomacromolecules. 2009;10(7):1810–7. doi:10.1021/bm900240k.

    Article  Google Scholar 

  19. Anokhina EB, Buravkova LB. Heterogeneity of stromal precursor cells isolated from rat bone marrow. Tsitologiia. 2007;49(1):40–7.

    Google Scholar 

  20. Huang Y, Siewe M, Madihally SV. Effect of spatial architecture on cellular colonization. Biotechnol Bioeng. 2006;93(1):64–75. doi:10.1002/bit.20703.

    Article  Google Scholar 

  21. Lai JY, Lin PK, Hsiue GH, Cheng HY, Huang SJ, Li YT. Low bloom strength gelatin as a carrier for potential use in retinal sheet encapsulation and transplantation. Biomacromolecules. 2009;10(2):310–9. doi:10.1021/bm801039n.

    Article  Google Scholar 

  22. Petrenko YA, Ivanov RV, Petrenko AY, Lozinsky VI. Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells. J Mater Sci Mater Med. 2011;22(6):1529–40. doi:10.1007/s10856-011-4323-6.

    Article  Google Scholar 

  23. Davis JM, ed. Basic cell culture. 2nd ed. Oxford University Press: Oxford, UK. 2002.

  24. Yudin VE, et al. Wet spinning of fibers made of chitosan and chitin nanofibrils. Carbohydr Polym. 2014;108:176–82. doi:10.1016/j.carbpol.2014.02.090.

    Article  Google Scholar 

  25. Kulichikhin VG, Semakov AV, Karbushev VV, Platé NA, Picken SJ. The chaos-to-order transition in critical modes of shearing for polymer and nanocomposite melts. Poly Sci Series A. 2009;51(11):1303–12. doi:10.1134/S0965545X09110169.

    Article  Google Scholar 

  26. Yin H, Mo D, Chen D. Orientation behavior of attapulgite nanoparticles in poly(acrylonitrile)/attapulgite solutions by rheological analysis. J Poly Sci Part B: Polym Phys. 2009;47(10):945–54. doi:10.1002/polb.21701.

    Article  Google Scholar 

  27. Balaban NQ, et al. Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nat Cell Biol. 2001;3(5):466–72. doi:10.1038/35074532.

    Article  Google Scholar 

  28. Okuyama K, Noguchi K, Hanafusa Y, Osawa K, Ogawa K. Structural study of anhydrous tendon chitosan obtained via chitosan/acetic acid complex. Int J Biol Macromol. 1999;26(4):285–93.

    Article  Google Scholar 

  29. Okuyama K, Noguchi K, Kanenari M, Egawa T, Osawa K, Ogawa K. Structural diversity of chitosan and its complexes. Carbohydr Poly. 2000;41(3):237–47. doi:10.1016/S0144-8617(99)00142-3.

    Article  Google Scholar 

  30. Zhang Y, Xue C, Xue Y, Gao R, Zhang X. Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction. Carbohydr Res. 2005;340(11):1914–7. doi:10.1016/j.carres.2005.05.005.

    Article  Google Scholar 

  31. Rinaudo M. Chitin and chitosan: properties and applications. Prog Polym Sci. 2006;31(7):603–32. doi:10.1016/j.progpolymsci.2006.06.001.

    Article  Google Scholar 

  32. Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials. 2003;24(24):4337–51.

    Article  Google Scholar 

  33. Zaborowska M, Bodin A, Backdahl H, Popp J, Goldstein A, Gatenholm P. Microporous bacterial cellulose as a potential scaffold for bone regeneration. Acta Biomater. 2010;6(7):2540–7. doi:10.1016/j.actbio.2010.01.004.

    Article  Google Scholar 

  34. Hillberg AL, Holmes CA, Tabrizian M. Effect of genipin cross-linking on the cellular adhesion properties of layer-by-layer assembled polyelectrolyte films. Biomaterials. 2009;30(27):4463–70. doi:10.1016/j.biomaterials.2009.05.026.

    Article  Google Scholar 

  35. Mendelsohn JD, Yang SY, Hiller J, Hochbaum AI, Rubner MF. Rational design of cytophilic and cytophobic polyelectrolyte multilayer thin films. Biomacromolecules. 2003;4(1):96–106. doi:10.1021/bm0256101.

    Article  Google Scholar 

Download references

Acknowledgements

VA Petrova, DD Chernyakov, and YA Skorik are grateful to the Russian Science Foundation (project #16-19-10536) for financial support.

Author information

Authors and Affiliations

  1. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya ul. 23, Kharkov, 61015, Ukraine

    Victoria V. Kiroshka, Yulia O. Bozhkova & Katerina V. Kiroshka

  2. Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation

    Valentina A. Petrova, Daniil D. Chernyakov, Yulia G. Baklagina & Yury A. Skorik

  3. Institute of Silicate Chemistry of the Russian Academy of Sciences, Adm. Makarova nab. 2, St. Petersburg, 199034, Russian Federation

    Dmitry P. Romanov

  4. Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg, 198504, Russian Federation

    Roman V. Kremnev

Authors
  1. Victoria V. Kiroshka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valentina A. Petrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniil D. Chernyakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yulia O. Bozhkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Katerina V. Kiroshka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yulia G. Baklagina
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dmitry P. Romanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Roman V. Kremnev
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yury A. Skorik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yury A. Skorik.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kiroshka, V.V., Petrova, V.A., Chernyakov, D.D. et al. Influence of chitosan-chitin nanofiber composites on cytoskeleton structure and the proliferation of rat bone marrow stromal cells. J Mater Sci: Mater Med 28, 21 (2017). https://doi.org/10.1007/s10856-016-5822-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10856-016-5822-2

Keywords

Search

Navigation