Advertisement

In vitro cyto-biocompatibility study of thin-film transistors substrates using an organotypic culture method

  • Eric Leclerc
  • Jean-Luc Duval
  • Christophe Egles
  • Satoshi Ihida
  • Hiroshi Toshiyoshi
  • Agnès Tixier-Mita
Biocompatibility Studies Original Research
Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

Thin-Film-Transistors Liquid-Crystal Display has become a standard in the field of displays. However, the structure of these devices presents interest not only in that field, but also for biomedical applications. One of the key components, called here TFT substrate, is a glass substrate with a dense and large array of thousands of transparent micro-electrodes that can be considered as a large scale multi-electrode array(s). Multi-electrode array(s) are widely used for in vitro electrical investigations on neurons and brain, allowing excitation, registration, and recording of their activity. However, the range of application of conventional multi-electrode array(s) is usually limited to some tens of cells in a homogeneous cell culture, because of a small area, small number and a low density of the micro-electrodes. TFT substrates do not have these limitations and the authors are currently studying the possibility to use TFT substrates as new tools for in vitro electrical investigation on tissues and organoids. In this respect, experiments to determine the cyto-biocompatibility of TFT substrates with tissues were conducted and are presented in this study. The investigation was performed using an organotypic culture method with explants of brain and liver tissues of chick embryos. The results in term of morphology, cell migration, cell density and adhesion were compared with the results from Thermanox®, a conventional plastic for cell culture, and with polydimethylsiloxane, a hydrophobic silicone. The results with TFT substrates showed similar results as for the Thermanox®, despite the TFT hydrophobicity. TFT substrates have a weak cell adhesion and promote cell migration similarly to Thermanox®. It could be concluded that the TFT substrates are cyto-biocompatible with the two studied organs.

Keywords

Contact Angle PMMA PDMS Complementary Metal Oxide Semiconductor Organotypic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

References

  1. 1.
    Gross GW, Rhoades BK, Azzazy HME, Wu MC. The use of neuronal networks on multi-electrode array(s) as biosensors. Biosens Bioelectron. 1995;10:553–67.CrossRefGoogle Scholar
  2. 2.
    Chopra KL, Major S, Pandya DK. Transparent conductors—a status review. Thin Solid Film. 1983;102(1):1–46.CrossRefGoogle Scholar
  3. 3.
    Ballini M, Muller J, Livi P, Yihui C, Frey U, Stettler A, Shadmani A, Viswam V, Lloyd Jones I, Jackel D, Radivojevic M, Lewandowska MK, Wei G, Fiscella M, Bakkum DJ, Heer F, Hierlemann A. A 1024-channel CMOS microelectrode array with 26,400 electrodes for recording and stimulation of electrogenic cells in vitro. IEEE J Solid-St Circ. 2014;49(11):2705–19.CrossRefGoogle Scholar
  4. 4.
    Kuo Y. Thin film transistor technology—past, present, and future. Electrochem Soc Interf. 2013;22(1):55–61.Google Scholar
  5. 5.
    Sterling J-D, Chen C, Nadim A. Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like. Patent US 2004/0231987 A1 2004.Google Scholar
  6. 6.
    Tixier-Mita A, Ségard B-D, Kim Y-J, Matsunaga Y, Fujita H and Toshiyoshi H. TFT Display panel technology as a base for biological cells electrical manipulation—Application to dielectrophoresis. The 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS’2015, 18–22 January 2015; Estoril, Portugal.Google Scholar
  7. 7.
    Wolff E, Haffen K. Sur une méthode de culture d’organes embryonnaires in vitro. Tex Rep Biol Med. 1952;10:463–72.Google Scholar
  8. 8.
    Sigot-Luizard MF, Lanfranchi M, Duval JL, Benslimane S, Sigot M, Guidoin RG, King MW. The cyto-compatibility of compound polyester-protein surfaces using an in vitro technique. In Vitro Cell Dev B. 1986;22:234CrossRefGoogle Scholar
  9. 9.
    Duval JL, Letort M, Sigot-Luizard MF. Comparative assessment of cell/substratum static adhesion using an in vitro organ culture method and computerized analysis system. Biomaterials. 1988;9:155–61.CrossRefGoogle Scholar
  10. 10.
    Duval JL, Dinis T, Vidal G, Vigneron P, Kaplan DL, Egles C. Organotypic culture to assess cell adhesion, growth and alignment of different organs on silk fibroin. J Tissue Eng Regen Med. 2014. doi: 10.1002/term.1916 Google Scholar
  11. 11.
    Leclerc E, Corlu A, Griscom L, Baudoin R, Legallais C. Guidance of liver and kidney organotypic cultures inside rectangular silicone microchannels. Biomaterials. 2006;27:4109–19.CrossRefGoogle Scholar
  12. 12.
    Anderson JR, Chiu DT, McDonald JC, Jackman RJ, Cherniavskaya O, Wu H, Whitesides S, Whitesides GM. Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. Anal Chem. 2000;72:3158–64.CrossRefGoogle Scholar
  13. 13.
    Charati SG, Stern SA. Diffusion of gases in silicone polymer: molecular dynamics simulations. Macromolecules. 1998;31:5529–35.CrossRefGoogle Scholar
  14. 14.
    Fujii T. PDMS-based microfluidic devices for biomedical applications. Microelectron Eng. 2002;61-62:907–14.CrossRefGoogle Scholar
  15. 15.
    Churaev NV. Contact Angles and surface forces. Adv Colloid Interface Sci. 1995;58:87–118.CrossRefGoogle Scholar
  16. 16.
    Leclerc E, Duval J-L, Pezron I, Nadaud F. Behaviors of liver and kidney explants from chicken embryos inside plasma treated PDMS microchannel. Mat Sci Eng C. 2009;29:861–68.CrossRefGoogle Scholar
  17. 17.
    Frazer RQ, Byron RT, Osborne PB, West KP. PMMA: an essential material in medicine and dentistry. J Long Term Eff Med Implants. 2005;15:629–39.CrossRefGoogle Scholar
  18. 18.
    Hwang IT, Ahn MY, Jung CH, Choi JH, Shin K. Micropatterning of mammalian cells on indium tin oxide substrates using ion implantation. J Biomed Nanotechnol. 2013;9:819–24.CrossRefGoogle Scholar
  19. 19.
    Selvakumaran J, Hughes MP, Keddie JL, Ewins DJ. Assessing biocompatibility of materials for implantable microelectrodes using cytotoxicity and protein adsorption studies. Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB. 2002; pp. 261–4Google Scholar
  20. 20.
    Leclerc E, Corlu A, Griscom L, Baudoin R, Legallais C. Guidance of liver and kidney organotypic cultures inside rectangular silicone microchannels. Biomaterials. 2006;27:4109–19.CrossRefGoogle Scholar
  21. 21.
    Leclerc E, Duval JL, Griscom L, Baudoin R, Legallais C. Selective control of liver and kidney cells migration during organotypic co-cultures inside fibronectin coated rectangular silicone microchannels. Biomaterials. 2007;28:1820–29.CrossRefGoogle Scholar
  22. 22.
    Leclerc E, Duval JL, Jalabert L. Comparison of the migration of liver and kidney explants inside trapezoidal PDMS microchannels. Mater Sci Eng C. 2010;30:1190–6.CrossRefGoogle Scholar
  23. 23.
    Dalton BA, Walboomers XF, Dziegielewski M, Evans MD, Taylor S, Jansen JA, Steele JG. Modulation of epithelial tissue and cell migration by microgrooves. J Biomed Mater Res. 2001;56:195–207.CrossRefGoogle Scholar
  24. 24.
    Anderson AS, Olsson P, Lidberg U, Sutherland D. The effects of continuous and discontinuous groove edges on cell shape and alignment. Exp Cell Res. 2003;288:177–88.CrossRefGoogle Scholar
  25. 25.
    Hamilton DW, Wong KS, Brunette DM. Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro. Calcif Tissue Int. 2006;78:314–25.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Sorbonne universités, Université de Technologie de Compiègne, CNRSCompiègne cedexFrance
  2. 2.Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial SciencesUniversity of TokyoMeguro-kuJapan
  3. 3.Department of Oral and Maxillofacial PathologyTufts University, School of Dental MedicineBostonUSA
  4. 4.Institute of Industrial SciencesThe University of TokyoMeguro-kuJapan

Personalised recommendations