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Cytocompatibility testing of cyclodextrin-functionalized antimicrobial textiles—a comprehensive approach

  • Biocompatibility Studies
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Functionalized textiles can be used in wound management to reduce the microbial burden in the wound area, to prevent wound infections, and to avoid cross-contamination between patients. In the present study, a comprehensive in vitro approach to enable the assessment of antibacterial activity of functionalized textiles and cytotoxicity of cyclodextrin (CD)-complexes with chlorhexidine diacetate (CHX), iodine (IOD), and polihexanide (PHMB) is suggested to evaluate their properties for supporting optimal conditions for wound healing. For all β-CD-antiseptic functionalized cotton samples a strong antibacterial effect on the Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis as well as on the Gram-negative bacteria Klebsiella pneumoniae and Escherichia coli was proven. In addition, β-CD-CHX and β-CD-PHMB were effective against the yeast Candida albicans. The growth of Pseudomonas aeruginosa could be reduced significantly by β-CD-IOD and β-CD-PHMB. The established comprehensive testing system for determination of biocompatibility on human HaCaT keratinocytes is suitable for obtaining robust data on cell viability, cytotoxicity and mode of cell death of the β-CD-antiseptic-complexes. The promising results of the high antimicrobial activity of these functionalized textiles show the high potential of such materials in medical applications.

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References

  1. White RJ, Cutting K, Kingsley A. Topical antimicrobials in the control of wound bioburden. Ostomy Wound Manag. 2006;52(8):26–58.

    Google Scholar 

  2. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev. 1999;12(1):147–79.

    Google Scholar 

  3. Wiegand C, Heinze T, Hipler UC. Comparative in vitro study on cytotoxicity, antimicrobial activity, and binding capacity for pathophysiological factors in chronic wounds of alginate and silver-containing alginate. Wound Repair Regen. 2009;17(4):511–21.

    Article  Google Scholar 

  4. Ip M, Lui SL, Poon VK, Lung I, Burd A. Antimicrobial activities of silver dressings: an in vitro comparison. J Med Microbiol. 2006;55(Pt 1):59–63.

    Article  Google Scholar 

  5. Hidalgo E, Bartolome R, Barroso C, Moreno A, Dominguez C. Silver nitrate: antimicrobial activity related to cytotoxicity in cultured human fibroblasts. Skin Pharmacol Appl. 1998;11(3):140–51.

    Article  Google Scholar 

  6. Szejtli J. Introduction and general overview of cyclodextrin chemistry. Chem Rev. 1998;98(5):1743–54.

    Article  Google Scholar 

  7. Del Valle EMM. Cyclodextrins and their uses: a review. Process Biochem. 2004;39(9):1033–46.

    Article  Google Scholar 

  8. Loftsson T, Duchene D. Cyclodextrins and their pharmaceutical applications. Int J Pharm. 2007;329(1–2):1–11.

    Article  Google Scholar 

  9. Stella VJ, He Q. Cyclodextrins. Toxicol Pathol. 2008;36(1):30–42.

    Article  Google Scholar 

  10. Kurkov SV, Loftsson T. Cyclodextrins. Int J Pharm. 2013;453(1):167–80.

    Article  Google Scholar 

  11. El Ghoul Y, Blanchemain N, Laurent T, Campagne C, El Achari A, Roudesli S, et al. Chemical, biological and microbiological evaluation of cyclodextrin finished polyamide inguinal meshes. Acta Biomater. 2008;4(5):1392–400.

    Article  Google Scholar 

  12. Hoang Thi TH, Chai F, Lepretre S, Blanchemain N, Martel B, Siepmann F, et al. Bone implants modified with cyclodextrin: study of drug release in bulk fluid and into agarose gel. Int J Pharm. 2010;400(1–2):74–85.

    Article  Google Scholar 

  13. Laurent T, Kacem I, Blanchemain N, Cazaux F, Neut C, Hildebrand HF, et al. Cyclodextrin and maltodextrin finishing of a polypropylene abdominal wall implant for the prolonged delivery of ciprofloxacin. Acta Biomater. 2011;7(8):3141–9.

    Article  Google Scholar 

  14. Buschmann HJ. Applications in the food and textile industries. In: Schneider HJ, editor. Applications of supramolecular chemistry. Boca Raton, FL: CRC Press; 2012. p. 417–34.

    Google Scholar 

  15. Fouda MM, Knittel D, Hipler UC, Elsner P, Schollmeyer E. Antimycotic influence of beta-cyclodextrin complexes - in vitro measurements using laser nephelometry in microtiter plates. Int J Pharm. 2006;311(1–2):113–21.

    Article  Google Scholar 

  16. Aleem O, Kuchekar B, Pore Y, Late S. Effect of beta-cyclodextrin and hydroxypropyl beta-cyclodextrin complexation on physicochemical properties and antimicrobial activity of cefdinir. J Pharmac Biomed. 2008;47(3):535–40.

    Article  Google Scholar 

  17. Teixeira KI, Denadai AM, Sinisterra RD, Cortes ME. Cyclodextrin modulates the cytotoxic effects of chlorhexidine on microrganisms and cells in vitro. Drug Deliv. 2015;22(3):444–53.

    Article  Google Scholar 

  18. Müller G, Kramer A. Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity. J Antimicrob Chemoth. 2008;61(6):1281–7.

    Article  Google Scholar 

  19. Müller G, Koburger T, Jethon FUW, Kramer A. Vergleich der bakterioziden Wirksamkeit und In-vitro-Zytotoxizität von Lavasept® und Prontosan®. GMS Krankenhaushygiene interdisziplinar. 2007;2(2):Doc42.

    Google Scholar 

  20. Kloth LC, Berman JE, Laatsch LJ, Kirchner PA. Bactericidal and cytotoxic effects of chloramine-T on wound pathogens and human fibroblasts in vitro. Advances in Skin & Wound Care. 2007;20(6):331–45.

    Article  Google Scholar 

  21. Wiegand C, Hipler UC. Evaluation of biocompatibility and cytotoxicity using keratinocyte and fibroblast cultures. Skin Pharmacol Physiol. 2009;22(2):74–82.

    Article  Google Scholar 

  22. Lindner K, Szente L, Szejtli J. Food flavoring with β-cyclodextrin complexed flavour substances. Acta Alimentaria. 1981;10:175–86.

    Google Scholar 

  23. Wang T, Li B, Feng Y, Guo Q. Preparation, quantitative analysis and bacteriostasis of solid state iodine inclusion complex with b-cyclodextrin. J Incl Phenom Macrocycl Chem. 2011;69(1):255–62.

    Article  Google Scholar 

  24. Reuscher H, Hirsenkorn R. Beta W7 MCT – New Ways in Surface Modification. Proc 8 Int Symp Cyclodextrins. 1996:553–558.

  25. Ameri Dehabadi V, Buschmann HJ, Gutmann JS. Spectrophotometric estimation of the accessible inclusion sites of b-cyclodextrin fixed on cotton fabrics using phenolic dyestuffs. Anal Methods. 2014;6:3382–7.

  26. Hipler UC, Schonfelder U, Hipler C, Elsner P. Influence of cyclodextrins on the proliferation of HaCaT keratinocytes in vitro. J Biomed Mater Res A. 2007;83(1):70–9.

    Article  Google Scholar 

  27. Niles AL, Moravec RA, Riss TL. Multiplex caspase activity and cytotoxicity assays. Methods Mol Biol. 2008;414:151–62.

    Google Scholar 

  28. Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL. A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal Biochem. 2007;366(2):197–206.

    Article  Google Scholar 

  29. Percival SL, Hill KE, Williams DW, Hooper SJ, Thomas DW, Costerton JW. A review of the scientific evidence for biofilms in wounds. Wound Repair Regen. 2012;20(5):647–57.

    Article  Google Scholar 

  30. Cabrera CE, Gómez RF, Zuñiga AE, Corral RH, López B, Chávez M. Epidemiology of nosocomial bacteria -resistant to antimicrobials. Colomb Med. 2011;42(1):117–25.

    Google Scholar 

  31. Finger S, Wiegand C, Buschmann HJ, Hipler UC. Antimicrobial properties of cyclodextrin-antiseptics-complexes determined by microplate laser nephelometry and ATP bioluminescence assay. Int J Pharm. 2012;436(1–2):851–6.

    Article  Google Scholar 

  32. Finger S, Wiegand C, Buschmann HJ, Hipler UC. Antibacterial properties of cyclodextrin-antiseptics-complexes determined by microplate laser nephelometry and ATP bioluminescence assay. Int J Pharm. 2013;452(1–2):188–93.

    Article  Google Scholar 

  33. Wlodkowic D, Telford W, Skommer J, Darzynkiewicz Z. Apoptosis and beyond: cytometry in studies of programmed cell death. Methods Cell Biol. 2011;103:55–98.

    Article  Google Scholar 

  34. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999;15:269–90.

    Article  Google Scholar 

  35. Schonfelder U, Radestock A, Elsner P, Hipler UC. Cyclodextrin-induced apoptosis in human keratinocytes is caspase-8 dependent and accompanied by mitochondrial cytochrome c release. Exp Dermatol. 2006;15(11):883–90.

    Article  Google Scholar 

  36. Wutzler P, Sauerbrei A, Klocking R, Brogmann B, Reimer K. Virucidal activity and cytotoxicity of the liposomal formulation of povidone-iodine. Antiviral Res. 2002;54(2):89–97.

    Article  Google Scholar 

  37. Shrivastava A, Tiwari M, Sinha RA, Kumar A, Balapure AK, Bajpai VK, et al. Molecular iodine induces caspase-independent apoptosis in human breast carcinoma cells involving the mitochondria-mediated pathway. J Biol Chem. 2006;281(28):19762–71.

    Article  Google Scholar 

  38. Liu XH, Chen GG, Vlantis AC, Tse GM, van Hasselt CA. Iodine induces apoptosis via regulating MAPKs-related p53, p21, and Bcl-xL in thyroid cancer cells. Mol Cell Endocrinol. 2010;320(1–2):128–35.

    Article  Google Scholar 

  39. Vitale M, Di Matola T, D’Ascoli F, Salzano S, Bogazzi F, Fenzi G, et al. Iodide excess induces apoptosis in thyroid cells through a p53-independent mechanism involving oxidative stress. Endocrinology. 2000;141(2):598–605.

    Google Scholar 

  40. Faria G, Cardoso CRB, Larson RE, Silva JS, Rossi MA. Chlorhexidine-induced apoptosis or necrosis in L929 fibroblasts: a role for endoplasmic reticulum stress. Toxicol Appl Pharm. 2009;234(2):256–65.

    Article  Google Scholar 

  41. Giannelli M, Chellini F, Margheri M, Tonelli P, Tani A. Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol In Vitro. 2008;22(2):308–17.

    Article  Google Scholar 

  42. Rohner E, Seeger JB, Hoff P, Dahn-Wollenberg S, Perka C, Matziolis G. Toxicity of polyhexanide and hydrogen peroxide on human chondrocytes in vitro. Orthopedics. 2011;34(7):e290–4.

    Google Scholar 

  43. Creppy EE, Diallo A, Moukha S, Eklu-Gadegbeku C, Cros D. Study of epigenetic properties of Poly(HexaMethylene Biguanide) hydrochloride (PHMB). Int J Environ Res Public Health. 2014;11(8):8069–92.

    Article  Google Scholar 

  44. Wiegand C, Abel M, Kramer A, Mueller G, Ruth P, Hipler UC. Proliferationsförderung und Biokompatibilität von Polihexanid. GMS Krankenhaushygiene Interdisziplinar. 2007;2(2):Doc43.

    Google Scholar 

  45. Niles AL, Moravec RA, Riss TL. In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screening. Curr Chem Genomics. 2009;3:33–41.

    Article  Google Scholar 

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Acknowledgments

The authors would like to acknowledge support of this work by 15997BG grant from Arbeitsgemeinschaft Industrieller Forschungsvereinigungen (AIF).

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Authors and Affiliations

  1. Department of Dermatology, University Hospital Jena, Jena, Germany

    Kirsten Reddersen, Susanne Finger, Michael Zieger, Cornelia Wiegand, Peter Elsner & Uta-Christina Hipler

  2. Deutsches Textilforschungszentrum Nord-West e.V. Krefeld, Krefeld, Germany

    Hans-Jürgen Buschmann

Authors
  1. Kirsten Reddersen
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  2. Susanne Finger
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  3. Michael Zieger
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  4. Cornelia Wiegand
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  5. Hans-Jürgen Buschmann
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  7. Uta-Christina Hipler
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Correspondence to Kirsten Reddersen.

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Reddersen, K., Finger, S., Zieger, M. et al. Cytocompatibility testing of cyclodextrin-functionalized antimicrobial textiles—a comprehensive approach. J Mater Sci: Mater Med 27, 190 (2016). https://doi.org/10.1007/s10856-016-5804-4

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  • DOI: https://doi.org/10.1007/s10856-016-5804-4

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