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Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Osteoporosis is a chronic disease that impairs proper bone remodeling. Guided bone regeneration is a surgical technique that improves bone defect in a particular region through new bone formation, using barrier materials (e.g. membranes) to protect the space adjacent to the bone defect. The polytetrafluorethylene membrane is widely used in guided bone regeneration, however, new membranes are being investigated. The purpose of this study was to evaluate the effect of P(VDFTrFE)/BT [poly(vinylidene fluoride-trifluoroethylene)/barium titanate] membrane on in vivo bone formation. Twenty-three Wistar rats were submitted to bilateral ovariectomy. Five animals were subjected to sham surgery. After 150 days, bone defects were created and filled with P(VDF-TrFE)/BT membrane or PTFE membrane (except for the sham and OVX groups). After 4 weeks, the animals were euthanized and calvaria samples were subjected to histomorphometric and computed microtomography analysis (microCT), besides real time polymerase chain reaction (real time PCR) to evaluate gene expression. The histomorphometric analysis showed that the animals that received the P(VDF-TrFE)/BT membrane presented morphometric parameters similar or even better compared to the animals that received the PTFE membrane. The comparison between groups showed that gene expression of RUNX2, BSP, OPN, OSX and RANKL were lower on P(VDF-TrFE)/BT membrane; the gene expression of ALP, OC, RANK and CTSK were similar and the gene expression of OPG, CALCR and MMP9 were higher when compared to PTFE. The results showed that the P(VDF-TrFE)/BT membrane favors bone formation, and therefore, may be considered a promising biomaterial to support bone repair in a situation of osteoporosis.

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Acknowledgments

Sebastião C. Bianco and Milla S. Tavares are acknowledged for technical assistance during the experiments.

Funding

This work was supported by São Paulo Research Foundation—FAPESP [grant number 2014/02984-0], National Council for Scientific and Technological [CNPq] and Coordination for the Improvement of Higher Education Personnel (CAPES). The synthesis of the PVDF-TrFE/BaTiO3 membranes was supported by Minas Gerais State Research Foundation (FAPEMIG, Brazil) under the Grant TEC - APQ-03013-15

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Authors and Affiliations

  1. Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil

    Priscilla Hakime Scalize, Karina F. Bombonato-Prado, Luiz Gustavo de Sousa, Adalberto Luiz Rosa, Marcio Mateus Beloti, Marisa Semprini, Adriana L. G. de Almeida, Fabíola Singaretti de Oliveira, Simone Cecilio Hallak Regalo & Selma Siessere

  2. Federal University of Itajubá (UNIFEI), Itajubá, Minas Gerais, Brazil

    Rossano Gimenes

Authors
  1. Priscilla Hakime Scalize
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  2. Karina F. Bombonato-Prado
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  3. Luiz Gustavo de Sousa
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  4. Adalberto Luiz Rosa
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  5. Marcio Mateus Beloti
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  6. Marisa Semprini
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  7. Rossano Gimenes
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  8. Adriana L. G. de Almeida
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  9. Fabíola Singaretti de Oliveira
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Correspondence to Selma Siessere.

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Scalize, P.H., Bombonato-Prado, K.F., de Sousa, L.G. et al. Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model. J Mater Sci: Mater Med 27, 180 (2016). https://doi.org/10.1007/s10856-016-5799-x

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