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Chemical stability and osteogenic activity of plasma-sprayed boron-modified calcium silicate-based coatings

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

In recent years, CaSiO3 bio-ceramic coatings have attracted great attention because of their good bioactivity. However, their high degradation rates in physiological environment restrict their practical applications. In this work, boron-modified CaSiO3 ceramic (Ca11Si4B2O22, B-CS) coating was developed on Ti substrates by plasma-spraying technique attempting to obtain enhanced chemical stability and osteogenic activity. The B-CS coating possessed significantly increased chemical stability due to the introduction of boron and consequently the modified crystal structure, while maintaining good bioactivity. Scanning electron microscope and immunofluorescence studies showed that better cellular adhesion and extinctive filopodia-like processes were observed on the B-CS coating. Compared with the pure CaSiO3 (CS) coating, the B-CS coating promoted MC3T3-E1 cells attachment and proliferation. In addition, enhanced collagen I (COL-I) secretion, alkaline phosphatase activity, and extracellular matrix mineralization levels were detected from the B-CS coating. According to RT-PCR results, notable up-regulation expressions of mineralized tissue-related genes, such as runt-related transcription factor 2 (Runx2), bone sialoprotein and osteocalcin, and bone morphogenetic protein 7 (BMP-7) were observed on the B-CS coating compared with the CS coating. The above results suggested that Ca11Si4B2O22 coatings possess excellent osteogenic activity and might be a promising candidate for orthopedic applications.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (Grant No. 51502328, No. 81301537, No. 81300917) and the Opening Project of the Shanghai Key Laboratory of Orthopedic Implant (Grant No. KFKT2016003).

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Authors and Affiliations

  1. Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China

    Xiang Lu, Kai Li, Youtao Xie, Liping Huang & Xuebin Zheng

  2. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, PR China

    Xiang Lu

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  1. Xiang Lu
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Correspondence to Kai Li or Xuebin Zheng.

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Lu, X., Li, K., Xie, Y. et al. Chemical stability and osteogenic activity of plasma-sprayed boron-modified calcium silicate-based coatings. J Mater Sci: Mater Med 27, 166 (2016). https://doi.org/10.1007/s10856-016-5781-7

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