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Bioprosthetic mesh of bacterial cellulose for treatment of abdominal muscle aponeurotic defect in rat model

  • Raquel Kelner Silveira
  • Antônio Roberto Barros Coelho
  • Flávia Cristina Morone Pinto
  • Amanda Vasconcelos de Albuquerque
  • Djalma Agripino de Melo Filho
  • José Lamartine de Andrade Aguiar
Biocompatibility Studies Original Research
Part of the following topical collections:
  1. Biocompatibility Studies

Abstract

The use of meshes for treatment of hernias continues to draw attention of surgeons and the industry in the search of an ideal prosthesis. The purpose of this work is to use meshes manufactured from bacterial cellulose, evaluate their organic tissue interaction and compare with an expanded polytetrafluorethylene (ePTFE’s) prosthesis used to repair acute defect of muscle aponeurotic induced in rats. Forty-five male Wistar rats were classified using the following criteria: (1) surgical repair of acute muscle aponeurotic defect with perforated bacterial cellulose film (PBC; n = 18); (2) compact bacterial cellulose film (CBC; n = 12) and (3) ePTFE; (n = 15). After postoperative period, rectangles (2 × 3 cm) including prosthesis, muscles and peritoneum were collected for biomechanical, histological and stereological analysis. In all cases, the maximum acceptable error probability for rejecting the null hypothesis was 5 %. Between PBC and CBC samples, the variables of strain (P = 0.011) and elasticity (P = 0.035) were statistically different. The same was found between CBC and ePTFE (elasticity, P = 0.000; strain, P = 0.009). PBC differed from CBC for giant cells (P = 0.001) and new blood vessels (P = 0.000). In conclusion, there was biological integration and biomechanical elasticity of PBC; therefore, we think this option should be considered as a new alternative biomaterial for use as a bio prosthesis.

Keywords

Sugarcane Giant Cell Collagen Fiber Bacterial Cellulose Polypropylene Mesh 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Research Performed at Center for Experimental Surgery of the Federal University of Pernambuco UFPE, Recife-PE, Brazil. Research Performed at collaboration with the Laboratory of Human Reproduction of the University of São Paulo (UNIFESP), Laboratory of Immunopathology Keizo Asami (LIKA), and the Department of Nuclear Energy (DEN) of the Federal University of Pernambuco, Recife/PE, Brazil. The English version of this text has been revised by a native speaker, Sidney Pratt, Canadian, BA, MAT (The Johns Hopkins University), RSA diploma (TEFL) University of Cambridge.

Financial source

National Council for Scientific and Technological Development – CNPq.

Compliance with ethical standards

Conflicts of interest

All authors have read the journal’s policy and there are no potential conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Raquel Kelner Silveira
    • 1
  • Antônio Roberto Barros Coelho
    • 1
  • Flávia Cristina Morone Pinto
    • 2
  • Amanda Vasconcelos de Albuquerque
    • 2
  • Djalma Agripino de Melo Filho
    • 1
  • José Lamartine de Andrade Aguiar
    • 1
  1. 1.Department of Surgery, Center for Health SciencesFederal University of Pernambuco, UFPERecifeBrazil
  2. 2.Center for Experimental Surgery, Department of Surgery, Center for Health SciencesFederal University of Pernambuco, UFPERecifeBrazil

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