Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility

  • Kun Zhang
  • Jun-ying Chen
  • Wei Qin
  • Jing-an Li
  • Fang-xia Guan
  • Nan Huang
Tissue Engineering Constructs and Cell Substrates Original Research
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates


The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.


Activate Partial Thromboplastin Time Collagen Coating Blood Compatibility Platelet Adhesion Test Fluid Flow Shear Stress 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was financially supported by the Funds of Key Basic Research Program (2011CB606204), the Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan province (U1504310), China Postdoctoral Science Foundation (2014M562333, 2015M582206), and Postdoctoral Scientific Research Fund of Henan Province (2014020).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kun Zhang
    • 1
    • 2
    • 3
  • Jun-ying Chen
    • 3
  • Wei Qin
    • 4
  • Jing-an Li
    • 3
  • Fang-xia Guan
    • 1
    • 2
  • Nan Huang
    • 3
  1. 1.School of Life ScienceZhengzhou UniversityZhengzhouPeople’s Republic of China
  2. 2.Center of Stem Cell and Regenerative MedicineFirst Affiliated Hospital of Zhengzhou UniversityZhengzhouPeople’s Republic of China
  3. 3.Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and EngineeringSouthwest Jiaotong UniversityChengduPeople’s Republic of China
  4. 4.Jiangsu Heze Allian Cells Bioscience Co., LtdChangzhouPeople’s Republic of China

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