Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility
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The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.
KeywordsActivate Partial Thromboplastin Time Collagen Coating Blood Compatibility Platelet Adhesion Test Fluid Flow Shear Stress
This work was financially supported by the Funds of Key Basic Research Program (2011CB606204), the Joint Fund for Fostering Talents of National Natural Science Foundation of China and Henan province (U1504310), China Postdoctoral Science Foundation (2014M562333, 2015M582206), and Postdoctoral Scientific Research Fund of Henan Province (2014020).
- 8.Enrico J, Oliver M, Siavosh M, Jandt E, Mutschke O, Mahboobi S, Uecker A, Platz R, Berndt A, Böhmer FD, Figulla HR, Werner GS. Stent-based release of a selective PDGF-receptor blocker from the bis-indolylmethanon class inhibits restenosis in the rabbit animal model. Vascul Pharmacol. 2010;52:55–62.CrossRefGoogle Scholar
- 20.Liu T, Liu Y, Chen Y, Liu SH, Maitz MF, Wang X, Zhang K, Wang J, Wang Y, Chen JY, Huang N. Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. Acta Biomater. 2014;2014(10):1940–4.CrossRefGoogle Scholar
- 22.Huang N, Leng YX, Ding PD. 17—Surface engineered titanium alloys for biomedical devices. Surf Eng Light Alloys. 2010;568–602.Google Scholar
- 25.Yang Y, Qi PK, Wen F, Li XY, Xia Q, Maitz MF, Yang ZL, Shen R, Tu QF, Huang N. Mussel-inspired one-step adherent coating rich in amine groups for covalent immobilization of heparin: hemocompatibility, growth behaviors of vascular cells, and tissue response. ACS Appl Mater Interfaces. 2014;6:14608–20.CrossRefGoogle Scholar
- 30.Zhang K, Li JA, Wang J, Liu T, Wang X, Chen JY, Huang N, Guan FX (2015). Combined REDV polypeptide and heparin onto titanium surface for the hemocompatibility and selectively endothelialization. J Cell Sci Therapy. 2015;6(1):1. doi:10.4172/2157-7013.1000198Google Scholar
- 31.Li JA, Zhang K, Wu F, He ZK, Yang P, Huang N. Constructing bio-functional layers of hyaluronan and type IV collagen on titanium surface for improving endothelialization. J Mater Sci. 2015;50:3226–36.Google Scholar
- 41.Li JA, Zhang K, Yang P, Qin W, Li GC, Zhao AS, Huang N. Human vascular endothelial cell morphology and functional cytokine secretion influenced by different size of HA micro-pattern on titanium substrate. Colloids Surf B. 2013;10:199–207.Google Scholar