Advertisement

Antibiotic-loaded chitosan–Laponite films for local drug delivery by titanium implants: cell proliferation and drug release studies

  • Farideh Ordikhani
  • Mehdi Dehghani
  • Arash Simchi
Delivery Systems Original Research
Part of the following topical collections:
  1. Delivery Systems

Abstract

In this study, chitosan–Laponite nanocomposite coatings with bone regenerative potential and controlled drug-release capacity are prepared by electrophoretic deposition technique. The controlled release of a glycopeptide drug, i.e. vancomycin, is attained by the intercalation of the polymer and drug macromolecules into silicate galleries. Fourier-transform infrared spectrometry reveals electrostatic interactions between the charged structure of clay and the amine and hydroxyl groups of chitosan and vancomycin, leading to a complex positively-charged system with high electrophoretic mobility. By applying electric field the charged particles are deposited on the surface of titanium foils and uniform chitosan films containing 25–55 wt% Laponite and 937–1655 µg/cm2 vancomycin are obtained. Nanocomposite films exhibit improved cell attachment with higher cell viability. Alkaline phosphatase assay reveals enhanced cell proliferation due the gradual dissolution of Laponite particles into the culture medium. In-vitro drug-release studies show lower release rate through a longer period for the nanocomposite compared to pristine chitosan.

Graphical Abstract

Keywords

Chitosan Composite Coating Nanocomposite Film Bioactive Glass Chitosan Film 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank funding support from the Grant Program of Sharif University of Technology (No. G930305) and Elite National Institute (No. ENL 5418).

Supplementary material

10856_2015_5606_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)

References

  1. 1.
    Shchipunov Y, Ivanova N, Silant’ev V. Bionanocomposites formed by in situ charged chitosan with clay. Green Chem. 2009;11:1758–61.CrossRefGoogle Scholar
  2. 2.
    Kevadiya BD, Joshi GV, Mody HM, Bajaj HC. Biopolymer–clay hydrogel composites as drug carrier: host–guest intercalation and in vitro release study of lidocaine hydrochloride. Appl Clay Sci. 2011;52:364–7.CrossRefGoogle Scholar
  3. 3.
    Ojijo V, Ray SS. Processing strategies in bionanocomposites. Prog Polym Sci. 2013;38:1543–89.CrossRefGoogle Scholar
  4. 4.
    Pongjanyakul T, Khunawattanakul W, Strachan CJ, Gordond KC, Puttipipatkhachorn S, Rades T. Characterization of chitosan–magnesium aluminum silicate nanocomposite films for buccal delivery of nicotine. Int J Biol Macromol. 2013;55:24–31.CrossRefGoogle Scholar
  5. 5.
    Croisier F, Jerome C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013;49(4):780–92.CrossRefGoogle Scholar
  6. 6.
    Sarasam A, Brown P, Khajotia S, Dmytryk J, Madihally S. Antibacterial activity of chitosan-based matrices on oral pathogens. J Mater Sci. 2008;19(3):1083–90. doi: 10.1007/s10856-007-3072-z.Google Scholar
  7. 7.
    Xie W, Xu P, Wang W, Liu Q. Preparation and antibacterial activity of a water-soluble chitosan derivative. Carbohydr Polym. 2002;50(1):35–40.CrossRefGoogle Scholar
  8. 8.
    Pattnaik S, Nethala S, Tripathi A, Saravanan S, Moorthi A, Selvamurugan N. Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering. Int J Biol Macromol. 2011;49(5):1167–72.CrossRefGoogle Scholar
  9. 9.
    Cai Q, Gu Z, Fu T, Liu Y, Song H, Li F. Kinetic study of chitosan degradation by an electrochemical process. Polym Bull. 2011;67(4):571–82.CrossRefGoogle Scholar
  10. 10.
    Sinha VR, Singla AK, Wadhawan S, Kaushik R, Kumria R, Bansal K, et al. Chitosan microspheres as a potential carrier for drugs. Int J Pharm. 2004;274(1–2):1–33.Google Scholar
  11. 11.
    Di Martino A, Sittinger M, Risbud MV. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials. 2005;26(30):5983–90.CrossRefGoogle Scholar
  12. 12.
    Bernkop-Schnürch A, Dünnhaupt S. Chitosan-based drug delivery systems. Eur J Pharm Biopharm. 2012;81:463–9.CrossRefGoogle Scholar
  13. 13.
    Peter M, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H, et al. Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications. Carbohydr Polym. 2010;79(2):284–9.CrossRefGoogle Scholar
  14. 14.
    Khunawattanakul W, Puttipipatkhachorn S, Rades T, Pongjanyakul T. Chitosan–magnesium aluminum silicate nanocomposite films: physicochemical characterization and drug permeability. Int J Pharm. 2010;393:219–29.CrossRefGoogle Scholar
  15. 15.
    Cojocariu A, Profire L, Aflori M, Vasile C. In vitro drug release from chitosan/Cloisite 15A hydrogels. Appl Clay Sci. 2012;57:1–9.CrossRefGoogle Scholar
  16. 16.
    Salcedo I, Aguzzi C, Sandri G, Bonferoni MC, Mori M, Cerezo P, et al. In vitro biocompatibility and mucoadhesion of montmorillonite chitosan nanocomposite: a new drug delivery. Appl Clay Sci. 2012;55:131–7.CrossRefGoogle Scholar
  17. 17.
    Jung H, Kim H-M, Choy YB, Hwang S-J, Choy J-H. Laponite-based nanohybrid for enhanced solubility and controlled release of itraconazole. Int J Pharm. 2008;349:28–290.CrossRefGoogle Scholar
  18. 18.
    Wang Q, Zhang J, Wang A. Alkali activation of halloysite for adsorption and release of ofloxacin. Appl Surf Sci. 2013;287:54–61.CrossRefGoogle Scholar
  19. 19.
    Fan Q, Shan D, Xue H, He Y, Cosnier S. Amperometric phenol biosensor based on laponite clay–chitosan nanocomposite matrix. Biosens Bioelectron. 2007;22:816–21.CrossRefGoogle Scholar
  20. 20.
    Gaharwar AK, Rivera CP, Wu C-J, Schmidt G. Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles. Acta Biomater. 2011;7:4139–48.CrossRefGoogle Scholar
  21. 21.
    Ghadiri M, Chrzanowski W, Lee WH, Fathi A, Dehghani F, Rohanizadeh R. Physico-chemical, mechanical and cytotoxicity characterizations of Laponite®/alginate nanocomposite. Appl Clay Sci. 2013;58:64–73.CrossRefGoogle Scholar
  22. 22.
    Gaharwar AK, Schexnailder PJ, Kline BP, Schmidt G. Assessment of using Laponite® cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater. 2011;7:568–77.CrossRefGoogle Scholar
  23. 23.
    Shi Q, Li Q, Shan D, Fan Q, Xue H. Biopolymer-clay nanoparticles composite system (Chitosan-laponite) for electrochemical sensing based on glucose oxidase. Mater Sci Eng C. 2008;28:1372–5.CrossRefGoogle Scholar
  24. 24.
    Yang H, Hua S, Wang W, Wang A. Composite hydrogel beads based on chitosan and laponite: preparation, swelling, and drug release behaviour. Iran Polym J. 2011;20(6):479–90.Google Scholar
  25. 25.
    Esposito S, Leone S. Prosthetic joint infections: microbiology, diagnosis, management and prevention. Int J Antimicrob Agents. 2008;32(4):287–93.CrossRefGoogle Scholar
  26. 26.
    Xie Z, Liu X, Jia W, Zhang C, Huang W, Wang J. Treatment of osteomyelitis and repair of bone defect by degradable bioactive borate glass releasing vancomycin. J Control. Release. 2009;139(2):118–26.CrossRefGoogle Scholar
  27. 27.
    Goodman SB, Yao Z, Keeney M, Yang F. The future of biologic coatings for orthopaedic implants. Biomaterials. 2013;34(13):3174–83.CrossRefGoogle Scholar
  28. 28.
    Corni I, Ryan MP, Boccaccini AR. Electrophoretic deposition: from traditional ceramics to nanotechnology. J Eur Ceram Soc. 2008;28(7):1353–67.CrossRefGoogle Scholar
  29. 29.
    Boccaccini AR, Keim S, Ma R, Li Y, Zhitomirsky I. Electrophoretic deposition of biomaterials. J R Soc Interface. 2010;7(Suppl 5):S581–613.CrossRefGoogle Scholar
  30. 30.
    Gebhardt F, Seuss S, Turhan MC, Hornberger H, Virtanen S, Boccaccini AR. Characterization of electrophoretic chitosan coatings on stainless steel. Mater Lett. 2012;66(1):302–4.CrossRefGoogle Scholar
  31. 31.
    Simchi A, Pishbin F, Boccaccini AR. Electrophoretic deposition of chitosan. Mater Lett. 2009;63(26):2253–6.CrossRefGoogle Scholar
  32. 32.
    Pishbin F, Simchi A, Ryan MP, Boccaccini AR. Electrophoretic deposition of chitosan/45S5 Bioglass® composite coatings for orthopaedic applications. Surf Coat Technol. 2011;205:5260–8.CrossRefGoogle Scholar
  33. 33.
    Ordikhani F, Tamjid E, Simchi A. Characterization and antibacterial performance of electrodeposited chitosan-vancomycin composite coatings for prevention of implant-associated infections. Mater Sci Eng C. 2014;41:240–8.CrossRefGoogle Scholar
  34. 34.
    Ordikhani F, Simchi A. Long-term antibiotic delivery by chitosan-based composite coatings with bone regenerative potential. Appl Surf Sci. 2014;317:56–66.CrossRefGoogle Scholar
  35. 35.
    Ordikhani F, Farani MR, Dehghani M, Tamjid E, Simchi A. Physicochemical and biological properties of electrodeposited graphene oxide/chitosan films with drug-eluting capacity. Carbon. 2015;84:91–102.CrossRefGoogle Scholar
  36. 36.
    Chakraborty SP, Pramanik P, Roy S. A review on emergence of antibioticresistant staphylococcus aureus and role of chitosan nanoparicle in drug delivery. Int J Life Sci Pharma Res. 2012;2:L96–115.CrossRefGoogle Scholar
  37. 37.
    Chakraborty SP, Sahu SK, Pramanik P, Roy S. In vitro antimicrobial activity of nanoconjugated vancomycin against drug resistant Staphylococcus aureus. Int J Pharm. 2012;436(1–2):659–76.CrossRefGoogle Scholar
  38. 38.
    Xu Y, Ren X, Hanna MA. Chitosan/clay nanocomposite film preparation and characterization. J Appl Polym Sci. 2006;99:1684–91.CrossRefGoogle Scholar
  39. 39.
    Besra L, Liu M. A review on fundamentals and applications of electrophoretic deposition (EPD). Prog Mater Sci. 2007;52(1):1–61.CrossRefGoogle Scholar
  40. 40.
    Yuan Q, Shah J, Hein S, Misra RDK. Controlled and extended drug release behavior of chitosan-based nanoparticle carrier. Acta Biomater. 2010;6(3):1140–8.CrossRefGoogle Scholar
  41. 41.
    Ponsonnet L, Reybier K, Jaffrezic N, Comte V, Lagneau C, Lissac M, et al. Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour. Mater Sci Eng C. 2003;23(4):551–60.CrossRefGoogle Scholar
  42. 42.
    Vlacic-Zischke J, Hamlet SM, Friis T, Tonetti MS, Ivanovski S. The influence of surface microroughness and hydrophilicity of titanium on the up-regulation of TGFβ/BMP signalling in osteoblasts. Biomaterials. 2011;32(3):665–71.CrossRefGoogle Scholar
  43. 43.
    Pishbin F, Mourino V, Gilchrist JB, McComb DW, Kreppel S, Salih V, et al. Single-step electrochemical deposition of antimicrobial orthopaedic coatings based on a bioactive glass/chitosan/nano-silver composite system. Acta Biomater. 2013;9(7):7469–79.CrossRefGoogle Scholar
  44. 44.
    Depan D, Kumar AP, Singh RP. Cell proliferation and controlled drug release studies of nanohybrids based on chitosan-g-lactic acid and montmorillonite. Acta Biomater. 2009;5:93–100.CrossRefGoogle Scholar
  45. 45.
    Gaharwar AK, Schexnailder PJ, Jin Q, Wu C-J, Schmidt G. Addition of chitosan to silicate cross-linked PEO for tuning osteoblast cell adhesion and mineralization. Appl Mater Interfaces. 2010;2:3119–27.CrossRefGoogle Scholar
  46. 46.
    Pon-On W, Charoenphandhu N, Teerapornpuntakit J, Thongbunchoo J, Krishnamra N, Tang IM. In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen. Mater Sci Eng C. 2013;33(3):1423–31.CrossRefGoogle Scholar
  47. 47.
    Cheng Y, Luo X, Bentley WE, Betz J, Rubloff GW, Buckhout-White S, et al. In situ quantitative visualization and characterization of chitosan electrodeposition with paired sidewall electrodes. Soft Matter. 2010;6:3177–83.CrossRefGoogle Scholar
  48. 48.
    Ghadiri M, Hau H, Chrzanowski W, Agus H, Rohanizadeh R. Laponite clay as a carrier for in situ delivery of tetracycline. RSC Adv. 2013;3:20193–201.CrossRefGoogle Scholar
  49. 49.
    Davies JE, Ajami E, Moineddin R, Mendes VC. The roles of different scale ranges of surface implant topography on the stability of the bone/implant interface. Biomaterials. 2013;34(14):3535–46.CrossRefGoogle Scholar
  50. 50.
    Mieszawska AJ, Fourligas N, Georgakoudi I, Ouhib NM, Belton DJ, Perry CC, et al. Osteoinductive silk-silica composite biomaterials for bone regeneration. Biomaterials. 2010;31(34):8902–10.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Farideh Ordikhani
    • 1
  • Mehdi Dehghani
    • 1
  • Arash Simchi
    • 1
    • 2
  1. 1.Department of Materials Science and EngineeringSharif University of TechnologyTehranIran
  2. 2.Institute for Nanoscience and NanotechnologySharif University of TechnologyTehranIran

Personalised recommendations