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Fabrication of thermo-sensitive complex micelles for reversible cell targeting

  • Biomaterials Synthesis and Characterization
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Abstract

To ideally solve the contradiction between enhanced cellular uptake and prolonged blood circulation, reversible targeting polymeric micelles based on the expanding and shrinking behavior of a temperature-responsive polymer were developed. The micelle contained a hydrophobic PCL core and a mixed shell consisting of poly(N-isopropylacrylamide) (PNIPAAm) and biotin-terminated poly(ethylene glycol) (Biotin-PEG), and its targeting ability could be switched on/off by temperature. The cellular uptake of the complex polymeric micelles was studied. The results from a quantitative enzyme-linked immunosorbent assay (ELISA) indicated that the surface biotin content increased by as much as 11.6-fold when the temperature increased above the lower critical solution temperature (LCST). More importantly, the ELISA confirmed that biotin-mediated targeting on the surface was reversibly switched on and off for at least five cycles. In addition, the results from quantitative flow cytometry and confocal spectroscopy indicated that the cellular uptake of the targeted micelles at temperatures above the LCST was much higher than that at temperatures below the LCST. This complex polymeric micelle with reversible targeting property could be a promising alternative for drug delivery.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (51433004), Natural Science Foundation of Tianjin (13JCYBJC25100), and PCSIRT (IRT1257). We thank Prof. Deling Kong, Nankai University, for his help with the whole cell experiments.

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  1. Key Laboratory of Functional Polymer Materials of Ministry of Education and Institute of Polymer Chemistry, Nankai University, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300071, China

    Yukun Wu, Chengling Yang, Quanyong Lai, Qian Zhang, Wei Wang & Zhi Yuan

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  1. Yukun Wu
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Correspondence to Zhi Yuan.

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Wu, Y., Yang, C., Lai, Q. et al. Fabrication of thermo-sensitive complex micelles for reversible cell targeting. J Mater Sci: Mater Med 26, 255 (2015). https://doi.org/10.1007/s10856-015-5584-2

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