Abstract
This study shows the potential risk of microfiltration between two different types of implant-abutment connections screwed at 45 Ncm: external and internal. For the first time the use of a mechanical artificial mouth is used with the values (compression and torsion loads with a frequency of 2 Hz) of the human chewing. The mechanical tests were performed with an artificial saliva at 37 °C. The microgap in the connection was measured by an Image Analysis software incorporated in a high resolution scanning electron microscopy. Implant connections were filled with methylene blue by using self-adjustable precision pipettes and the quantity of leakage was determined by high sensitivity spectometry. We showed that the internal connection has lower microgaps compared to the external ones and these microgaps increased with the number of mechanical cycles. The leakage of methylene blue was higher when the external connection was performed. Microgaps and the influence of the mechanical loads are very important for the long-term behavior avoiding the bacteria colonization in the dental implants. These aspects should be known by the implantologists.
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Wagenberg B, Froum SJ. Prospective study of 94 platform-switched implants observed from 1992 to 2006. Int J Periodontics Restor Dent. 2010;30:9–17.
Tarnow DP, Cho SC, Wallace SS. The effect of inter-implant distance on the height of the inter-implant bone crest. J Periodontol. 2000;71:546–9.
Albrektsson T, Brånemark PI, Hansson HA, Lindström J. Acta Orthop Scand. 1981;52:155–70.
Steinebrunner L, Wolfart S, Bossmann K, Kern M. In vitro evaluation of bacterial leakage along the internal implant-34 abutment interface implant-abutment interface of different implant systems. Int J Oral Maxillofac Implants. 2005;20:875–81.
Quirynen M, Bollen CM, Eyssen H, van Steenberghe D. Microbial penetration along the implant components of the Brånemark system. An in vitro study. Clin Oral Implants Res. 1994;5(4):239–44.
Broggini N, Mcmanus LM, Hermann JS. Persistent acute inflamation at the implant-abutment interface. J Dent Res. 2003;82:232–7.
Adell R, Lekholm U, Rockler B, Brånemark P-J. A 15-year study of osseintegrated implants in the treatment of the edentulous jaw. Int J Oral Surg. 1981;10:387–416.
Quirynen M, van Steenberghe D. Bacterial colonization of the internal part of two –stage implants. An in vitro study. Clin Oral Implants Res. 1993;4:158–61.
Traversy MC, Birek P. Fluid and microbial leakage of implant abutment assembly in vitro. J Dent Res. 1992;71:754.
Ericsson I, Persson LG, Berglundh T, Marinello CP, Lindhe J, Kinge B. Different types of inflamatory reactions in periimplant soft tissues. J Clin Periodontol. 1995;22:255–61.
Broggini N, McManus LM, Hermann JS. Periimplant inflamation defined by the implant-abutment interface. J Dent Res. 2006;85:473–8.
Hermann JS, Schoolfied JD, Schenk RK, Buser D, Cochran DL. Influence of the size of the microgap on crestal bone changes around titanium implants. A histometric evaluation of unloaded non-submerged implants in the canine mandible. J Periodontol. 2001;72:1372–83.
Tesmer M, Wallet S, Koutouzis T, Lundgren T. Bacterial colonization of the dental implant fixture-abutment interface: an in vitro study. J Periodontol. 2009;80(12):1991–7.
Texeira W, Ribeiro RF, Sato S, Pedrazzi V. Microleakage into and from two-stage implants: an in vitro comparative study. Int J Oral Maxillofac Implants. 2011;26:56–62.
Koutouzis T, Wallet S, Calderon N, Lundgren T. Bacterial colonization of the implant-abutment interface using an in vitro dynamic loading model. J Periodontol. 2011;82(4):613–8.
Volker K, Jansen J. Microbial leakage and marginal fit of the implant- abutment interface. Int J Maxillofac Implants. 1997;12:527–40.
Do Nascimento C, Miani PK, Pedrazzi V, Gonçalves RB, Ribeiro RF, Faria AC, Macedo AP, de Albuquerque RF Jr. Leakage of saliva through the implant-abutment interface: in vitro evaluation of three different implant connections under unloaded and loaded conditions. Int J Oral Maxillofac Implants. 2012;27(3):551–60.
Jaworski ME, Melo AC, Picheth CM, Sartori IA. Analysis of the bacterial seal at the implant-abutment interface in external-hexagon and morse taper-connection implants: an in vitro study using a new methodology. Int J Oral Maxillofac Implants. 2012;27(5):1091–5.
Berglundh T, Abrahamsson I, Lindhe J. Bone reactions to longstanding functional load at implants: an experimental study in dogs. J Clin Periodontol. 2005;32:925–32.
Laurell L, Lundgren D. Marginal bone level changes at dental implants after 5 years in function: a meta-analysis. Clin Impl Dent Relat Res. 2011;13:19–28.
Aloise JP, Curcio R, Laporta MZ, Rossi L, da Silva AM, Rapoport A. Microbial leakage throught the implant- abutment interface of Morse taper implants in vitro. Clin Oral Implants Res. 2010;21:328–35.
Gross M, Abramovich I, Weiss EI. Microleakage at the abutment-implant interface of osseointegrated implants: a comparative study. Int J Oral Maxillofac Implants. 1999;14:94.
Binon PP. The effect of implant/abutment hexagonal misfit on screw joint stability. Int J Prosthodont. 1996;9:149–60.
Ranger B, Gunne J, Sullivan DY. Mechanical aspects of a Bränemark implant connected to a natural tooth: an in vitro study. Int J Oral Maxillofac Implants. 1991;6:177–86.
Zipprich H, Weigl P, Lange B, Lauer H-C. Micromovements at the implant-abutment interface: measurement, causes and consequences (in German). Implantologie. 2007;15:31–45.
Assenza B, Tripodi D, Scarano A, Perrotti V, Piattelli A, Iezzi G, D’Ercole S. Bacterial leakage in implants with different implant-abutment connections: an in vitro study. J Periodontol. 2012;83(4):491–7.
Coelho PG, Sudack P, Suzuki M, Kurtz KS, Romanos GE, Silva NR. In vitro evaluation of the implant abutment connection sealing capability of different implant systems. J Oral Rehabil. 2008;35(12):917–24.
Acknowledgments
This work was supported by CICYT Spanish project MAT-2012-30706 and the Andorra and Catalan Government for CTP grants. The authors are grateful to Soadco® for your support.
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The authors have not financial relationship with the organization that sponsored the research. The authors have full control of all primary data and that they agree to allow the journal to review their data if requested.
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Martin-Gili, D., Molmeneu, M., Fernandez, M. et al. Determination of fluid leakages in the different screw-retained implant-abutment connections in a mechanical artificial mouth. J Mater Sci: Mater Med 26, 211 (2015). https://doi.org/10.1007/s10856-015-5544-x
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DOI: https://doi.org/10.1007/s10856-015-5544-x