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The grafting of a thin layer of poly(sodium styrene sulfonate) onto poly(ε-caprolactone) surface can enhance fibroblast behavior

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Poly(sodium styrene sulfonate) (pNaSS) was grafted onto poly(ε-caprolatone) (PCL) surfaces via ozonation and graft polymerization. The effect of ozonation and polymerization time, as well as the Mohr’s salt concentration in the grafting solution, on the degree of grafting was investigated. The degree of grafting was determined through toluidine blue staining. The surface chemical change was characterized by attenuated total reflection Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The result demonstrated that the grafting did not induce any degradation of PCL, and that pNaSS was grafted onto PCL as a thin and covalently stable layer. Furthermore, the modified PCL surface reveals a significant increase in the metabolic activity of fibroblastic cells, as well as a better cell spreading with higher adhesion strength. Consequently, bioactivity of PCL is greatly enhanced by immobilizing a thin layer of pNaSS onto its surface. The grafting of pNaSS is a promising approach to increase the bioactivity of PCL-based materials used in tissue engineering applications, such as ligament reconstruction.

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Acknowledgments

The authors thank the Ministry of National Education, Research and Technology for the MENRT scholarship granted to Stéphane Huot. The XPS experiments were done at NESAC/Bio, which is funded by grant EB-002027 from the US National Institutes of Health. The authors have no conflict of interest to disclose.

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Authors and Affiliations

  1. Université Paris 13, Sorbonne Paris Cité, Laboratoire de Chimie, Structures, Propriétés de Biomatériaux et d’Agents Thérapeutiques (CSPBAT), CNRS UMR 7244, 93430, Villetaneuse, France

    Géraldine Rohman, Stéphane Huot, Maria Vilas-Boas, Gabriela Radu-Bostan & Véronique Migonney

  2. National ESCA and Surface Analysis Center for Biomedical Problems (NESAC/Bio), Departments of Bioengineering and Chemical Engineering, University of Washington, Box 351653, Seattle, WA, USA

    David G. Castner

Authors
  1. Géraldine Rohman
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  2. Stéphane Huot
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  3. Maria Vilas-Boas
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  4. Gabriela Radu-Bostan
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  5. David G. Castner
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  6. Véronique Migonney
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Correspondence to Géraldine Rohman.

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Rohman, G., Huot, S., Vilas-Boas, M. et al. The grafting of a thin layer of poly(sodium styrene sulfonate) onto poly(ε-caprolactone) surface can enhance fibroblast behavior. J Mater Sci: Mater Med 26, 206 (2015). https://doi.org/10.1007/s10856-015-5539-7

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  • DOI: https://doi.org/10.1007/s10856-015-5539-7

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