A combined method for bilayered vascular graft fabrication

  • Tamer Al Kayal
  • Devid Maniglio
  • Walter Bonani
  • Paola Losi
  • Claudio Migliaresi
  • Giorgio Soldani
Tissue Engineering Constructs and Cell Substrates Rapid Communication
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates


Autologous saphenous vein is still the conduit of choice for peripheral by-pass. Synthetic vascular grafts in polyethylene terephthalate and expanded polytetrafluoroethylene are used if vein access cannot be obtained. However they are successfully used to replace large diameter vessels, but they fail in small diameters (<6 mm). In the present study a bilayered synthetic vascular graft was developed. The graft was composed of an inner nanofibrous layer obtained by electrospinning able to host endothelial cells and a highly porous external layer obtained by spray, phase-inversion technique capable to sustain tunica media regeneration. Graft morphology and thickness, fiber size, pore size and layer adhesion strength were assessed. The innovative combination of two different consolidated techniques allowed to manufacture a nanostructured composite graft featuring a homogeneous microporous layer firmly attached on the top of the electrospun layer. By tuning the mechanical properties and the porosity of vascular prostheses, it will be possible to optimize the graft for in situ tissue regeneration while preventing blood leakage.

Graphical Abstract


Vascular Graft Peel Test Polycarbonate Urethane Synthetic Vascular Graft Graft Morphology 
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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Tamer Al Kayal
    • 1
  • Devid Maniglio
    • 2
    • 3
  • Walter Bonani
    • 2
    • 3
  • Paola Losi
    • 1
  • Claudio Migliaresi
    • 2
    • 3
  • Giorgio Soldani
    • 1
  1. 1.Institute of Clinical PhysiologyNational Research CouncilMassaItaly
  2. 2.Department of Industrial Engineering and BIOtech Research CentreUniversity of TrentoTrentoItaly
  3. 3.Trento Research UnitEuropean Institute of Excellence on Tissue Engineering and Regenerative Medicine and INSTM Research CenterTrentoItaly

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