Advertisement

A combined method for bilayered vascular graft fabrication

  • Tamer Al Kayal
  • Devid Maniglio
  • Walter Bonani
  • Paola Losi
  • Claudio Migliaresi
  • Giorgio Soldani
Tissue Engineering Constructs and Cell Substrates Rapid Communication
Part of the following topical collections:
  1. Tissue Engineering Constructs and Cell Substrates

Abstract

Autologous saphenous vein is still the conduit of choice for peripheral by-pass. Synthetic vascular grafts in polyethylene terephthalate and expanded polytetrafluoroethylene are used if vein access cannot be obtained. However they are successfully used to replace large diameter vessels, but they fail in small diameters (<6 mm). In the present study a bilayered synthetic vascular graft was developed. The graft was composed of an inner nanofibrous layer obtained by electrospinning able to host endothelial cells and a highly porous external layer obtained by spray, phase-inversion technique capable to sustain tunica media regeneration. Graft morphology and thickness, fiber size, pore size and layer adhesion strength were assessed. The innovative combination of two different consolidated techniques allowed to manufacture a nanostructured composite graft featuring a homogeneous microporous layer firmly attached on the top of the electrospun layer. By tuning the mechanical properties and the porosity of vascular prostheses, it will be possible to optimize the graft for in situ tissue regeneration while preventing blood leakage.

Graphical Abstract

Keywords

Vascular Graft Peel Test Polycarbonate Urethane Synthetic Vascular Graft Graft Morphology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Liu S, Dong C, Lu G, Lu Q, Li Z, Kaplan DL, Zhu H. Bilayered vascular grafts based on silk proteins. Acta Biomater. 2013;9:8991–9003.CrossRefGoogle Scholar
  2. 2.
    Madhavan K, Elliott WH, Bonani W, Monnet E, Tan W. Mechanical and biocompatible characterizations of a readily available multilayer vascular graft. J Biomed Mater Res B Appl Biomater. 2013;101:506–19.CrossRefGoogle Scholar
  3. 3.
    Soletti L, Hong Y, Guan J, Stankus JJ, El-Kurdi MS, Wagner WR, Vorp DA. A bilayered elastomeric scaffold for tissue engineering of small diameter vascular grafts. Acta Biomater. 2010;6:110–22.CrossRefGoogle Scholar
  4. 4.
    de Valence S, Tille JC, Giliberto JP, Mrowczynski W, Gurny R, Walpoth BH, Möller M. Advantages of bilayered vascular grafts for surgical applicability and tissue regeneration. Acta Biomater. 2012;8:3914–20.CrossRefGoogle Scholar
  5. 5.
    Bondar B, Fuchs S, Motta A, Migliaresi C, Kirkpatrick CJ. Functionality of endothelial cells on silk fibroin nets: comparative study of micro- and nanometric fibre size. Biomaterials. 2008;29:561–72.CrossRefGoogle Scholar
  6. 6.
    Zonari A, Novikoff S, Electo NR, Breyner NM, Gomes DA, Martins A, et al. Endothelial differentiation of human stem cells seeded onto electrospun polyhydroxybutyrate-polyhydroxybutyrate-co-hydroxyvalerate fiber mesh. PLoS One. 2012;7:e35422.CrossRefGoogle Scholar
  7. 7.
    Cattaneo I, Figliuzzi M, Azzollini N, Catto V, Farè S, Tanzi MC, Alessandrino A, et al. In vivo regeneration of elastic lamina on fibroin biodegradable vascular scaffold. Int J Artif Organs. 2013;36:166–74.CrossRefGoogle Scholar
  8. 8.
    Wang Z, Cui Y, Wang J, Yang X, Wu Y, Wang K, et al. The effect of thick fibers and large pores of electrospun poly(ε-caprolactone) vascular grafts on macrophage polarization and arterial regeneration. Biomaterials. 2014;35:5700–10.CrossRefGoogle Scholar
  9. 9.
    Bergmeister H, Schreiber C, Grasl C, Walter I, Plasenzotti R, Stoiber M, et al. Healing characteristics of electrospun polyurethane grafts with various porosities. Acta Biomater. 2013;9:6032–40.CrossRefGoogle Scholar
  10. 10.
    Soldani G, Losi P, Bernabei M, Burchielli S, Chiappino D, Kull S, et al. Long term performance of small-diameter vascular grafts made of a poly(ether)urethane-polydimethylsiloxane semi-interpenetrating polymeric network. Biomaterials. 2010;31:2592–605.CrossRefGoogle Scholar
  11. 11.
    Khorasani MT, Shorgashti S. Fabrication of microporous polyurethane by spray phase inversion method as small diameter vascular grafts material. J Biomed Mater Res A. 2006;77:253–60.CrossRefGoogle Scholar
  12. 12.
    Bonani W, Maniglio D, Motta A, Tan W, Migliaresi C. Biohybrid nanofiber constructs with anisotropic biomechanical properties. J Biomed Mater Res B Appl Biomater. 2011;96:276–86.CrossRefGoogle Scholar
  13. 13.
    Vasita R, Katti DS. Nanofibers and their applications in tissue engineering. Int J Nanomedicine. 2006;1:15–30.CrossRefGoogle Scholar
  14. 14.
    Sell SA, McClure MJ, Garg K, Wolfe PS, Bowlin GL. Electrospinning of collagen/biopolymers for regenerative medicine and cardiovascular tissue engineering. Adv Drug Deliv Rev. 2009;5:1007–19.CrossRefGoogle Scholar
  15. 15.
    Bezuidenhout D, Williams DF, Zilla P. Polymeric heart valves for surgical implantation, catheter-based technologies and heart assist devices. Biomaterials. 2014;36C:6–25.Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Tamer Al Kayal
    • 1
  • Devid Maniglio
    • 2
    • 3
  • Walter Bonani
    • 2
    • 3
  • Paola Losi
    • 1
  • Claudio Migliaresi
    • 2
    • 3
  • Giorgio Soldani
    • 1
  1. 1.Institute of Clinical PhysiologyNational Research CouncilMassaItaly
  2. 2.Department of Industrial Engineering and BIOtech Research CentreUniversity of TrentoTrentoItaly
  3. 3.Trento Research UnitEuropean Institute of Excellence on Tissue Engineering and Regenerative Medicine and INSTM Research CenterTrentoItaly

Personalised recommendations