In situ synthesis of hydroxyapatite nanocomposites using iron oxide nanofluids at ambient conditions
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This paper describes a simple method for the room temperature synthesis of magnetite/hydroxyapatite composite nanocomposites using ferrofluids. The in situ synthesis of magnetic–hydroxyapatite results in a homogenous distribution of the two phases as seen both in transmission electron micrographs and assembled to a micron range in the confocal micrographs. The selected area diffraction pattern analysis shows the presence of both phases of iron oxide and hydroxyapatite. To the dialyzed ferrofluid, the constituents of hydroxyapatite synthesis was added, the presence of the superparamagnetic iron oxide particles imparts directionality to the hydroxyapatite crystal growth. Electron probe microanalysis confirms the co-existence of both iron and calcium atoms. Vibrating Sample magnetometer data shows magnetization three times more than the parent ferrofluid, the local concentration of iron oxide nanoparticles affects the strength of dipolar interparticle interactions changing the energy barrier for determining the collective magnetic behavior of the sample. The limitations inherent to the use of external magnetic fields which can be circumvented by the introduction of internal magnets located in the proximity of the target by a minimal surgery or by using a superparamagnetic scaffold under the influence of externally applied magnetic field inspires us to increase the magnetization of our samples. The composite in addition shows anti-bacterial properties against the two gram (−ve) bacteria tested. This work is significant as magnetite–hydroxyapatite composites are attracting a lot of attention as adsorbents, catalysts, hyperthermia agents and even as regenerative medicine.
KeywordsHydroxyapatite Iron Oxide Nanoparticles Differential Interference Contrast Calcium Nitrate Rapid Weight Loss
The authors acknowledge CSIR, Ministry of Science and Technology, Govt. of India for providing financial assistance from the network project “ESC-0103” and “OLP-0230”. Lubna acknowledges Department of Science & Technology (DST), Govt. of India for providing INSPIRE fellowship.
- 2.Moroz P, Jones SK, Gray BN. Magnetically mediated hyperthermia: current status and future directions. Int J Hyperth. 2002;18:267–84.Google Scholar
- 19.Cornell RM, Schwertmann U. The iron oxides. VCH verlagesellschaft: Federal Republic of Germany; 1996.Google Scholar
- 23.Mann S. Biomimetic materials chemistry. New York: VCH publishers, Inc.; 1996.Google Scholar
- 30.Predoi D, Barsan M, Andronescu E, Vatasescu-Balcan RA, Costache M. Hydroxyapatite-iron oxide bioceramic prepared using nano-size powders. J Optoelectron Adv Mater. 2007;9(11):3609–13.Google Scholar
- 31.Hoppe CE, Rivadulla F, Vidal-Vidal J, Lopez-Quintela MA, Rivas J. Magnetic relaxation of gamma Fe2O3 nanoparticles arrangements and electronic phase-segregated. J Nanosci Nanotechnol. 2008;8:2883–90.Google Scholar
- 32.Klabunde KJ, Mulukutla RS. Nanoscale materials in chemistry, chap. 7. New York: Wiley Interscience; 2001. p. 223–61.Google Scholar
- 33.Tran N, Mir A, Mallik D, Sinha A, Nayar S, Webster TJ. Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus. Int J Nanomed. 2010;5:277–83.Google Scholar
- 34.Panseri S, Cunha C, D’Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A. Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect. PLoS One. 2012;7(6):e38710.CrossRefGoogle Scholar