Journal of Materials Science: Materials in Medicine

, Volume 25, Issue 11, pp 2501–2512 | Cite as

Hybrid nanostructured Ag/ZnO decorated powder cellulose fillers for medical plastics with enhanced surface antibacterial activity

  • Pavel Bazant
  • Ivo Kuritka
  • Lukas Munster
  • Michal Machovsky
  • Zuzana Kozakova
  • Petr Saha


Hybrid inorganic–organic fillers based on nanostructured silver/zinc oxide decorations on micro-cellulose carrier particles were prepared by stepwise microwave assisted hydrothermal synthesis using soluble salts as precursors of silver and zinc oxide. Hexamethylenetetramine was used as precipitating agent for zinc oxide and reducing agent for silver. The inorganics covered all available surfaces of the cellulose particles with a morphology resembling a coral reef. Prepared particulate fillers were compounded to medical grade poly(vinyl chloride) matrix. Scanning electron microscopy and powder X-ray diffractometry were used to investigate the morphology and crystalline phase structure of fillers. The scanning electron microscopy was used for morphological study of composites. With respect to prospective application, the composites were tested on electrical and antibacterial properties. A small effect of water absorption in polymer composites on their dielectric properties was observed but no adverse effect of water exposure on prepared materials was manifested. Electrical conductivity of fillers and composites was measured and no influence of water soaking of composites was found at all. The surface antibacterial activity of prepared composites was evaluated according to the standard ISO 22196. Excellent performance against Escherichia coli and very high against Staphylococcus aureus was achieved.


Polymer Matrix Silver Nanoparticles Cellulose Powder Dielectric Spectrum Prepared Composite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This article was written with support of Operational Program Research and Development for Innovations co-funded by the European Regional Development Fund (ERDF) and national budget of Czech Republic, within the framework of project Centre of Polymer Systems (reg. number: CZ.1.05/2.1.00/03.0111). The authors also acknowledge the support of Operational Program Education for Competitiveness co-funded by the European Social Fund (ESF) and national budget of Czech Republic, within the framework of project Advanced Theoretical and Experimental Studies of Polymer Systems (reg. number: CZ.1.07/2.3.00/20.0104). The work of L. M. was supported by the Internal Grant Agency of Tomas Bata University in Zlin; contract Grant Number: IGA/FT/2014/008.

Supplementary material

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Supplementary material 1 (DOCX 112 kb)


  1. 1.
    Vroman I, Tighzert L. Biodegradable polymers. Materials. 2009;2(2):307–44.CrossRefGoogle Scholar
  2. 2.
    Sannino A, Demitri C, Madaghiele M. Biodegradable cellulose-based hydrogels: design and applications. Materials. 2009;2(2):353–73.CrossRefGoogle Scholar
  3. 3.
    Sirolli V, Di Stante S, Stuard S, Di Liberato L, Amoroso L, Cappelli P, et al. Biocompatibility and functional performance of a polyethylene glycol acid-grafted cellulosic membrane for hemodialysis. Int J Artif Organs. 2000;23(6):356–64.Google Scholar
  4. 4.
    Kwon JW, Yoon SH, Lee SS, Seo KW, Shim IW. Preparation of silver nanoparticles in cellulose acetate polymer and the reaction chemistry of silver complexes in the polymer. Bull Korean Chem Soc. 2005;26(5):837–40.CrossRefGoogle Scholar
  5. 5.
    Silva AR, Unali G. Controlled silver delivery by silver-cellulose nanocomposites prepared by a one-pot green synthesis assisted by microwaves. Nanotechnology. 2011;22(31):315605.CrossRefGoogle Scholar
  6. 6.
    Siqueira G, Bras J, Dufresne A. Cellulosic bionanocomposites: a review of preparation, properties and applications. Polymers. 2010;2(4):728–65.CrossRefGoogle Scholar
  7. 7.
    Stenstad P, Andresen M, Tanem BS, Stenius P. Chemical surface modifications of microfibrillated cellulose. Cellulose. 2008;15(1):35–45.CrossRefGoogle Scholar
  8. 8.
    Goncalves G, Marques PAAP, Neto CP, Trindade T, Peres M, Monteiro T. Growth, structural, and optical characterization of ZnO-coated cellulosic fibers. Cryst Growth Des. 2009;9(1):386–90.CrossRefGoogle Scholar
  9. 9.
    Singh AV, Rahman A, Kumar N, Aditi AS, Galluzzi M, Bovio S, et al. Bio-inspired approaches to design smart fabrics. Mater Des. 2012;36:829–39.CrossRefGoogle Scholar
  10. 10.
    Wiesner MR, Lowry GV, Alvarez P, Dionysiou D, Biswas P. Assessing the risks of manufactured nanomaterials. Environ Sci Technol. 2006;40(14):4336–45.CrossRefGoogle Scholar
  11. 11.
    Dastjerdi R, Montazer M. A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties. Colloids Surf B Biointerfaces. 2010;79(1):5–18.CrossRefGoogle Scholar
  12. 12.
    Sastri VS. Plastics in medical devices: properties, requirements and applications. Norwich: Elsevier/William Andrew; 2010.Google Scholar
  13. 13.
    Cioffi N, Rai M. Nano-antimicrobials: progress and prospects. Berlin: Springer; 2012.CrossRefGoogle Scholar
  14. 14.
    von Eiff C, Jansen B, Kohnen W, Becker K. Infections associated with medical devices—pathogenesis, management and prophylaxis. Drugs. 2005;65(2):179–214.CrossRefGoogle Scholar
  15. 15.
    Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16(10):2346–53.CrossRefGoogle Scholar
  16. 16.
    Sedlarik V. Antimicrobial modifications of polymers. In: Chamy R, Rosenkranz F, editors. Biodegradation—life of science. Croatia: InTech; 2013.Google Scholar
  17. 17.
    Panacek A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N, et al. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B. 2006;110(33):16248–53.CrossRefGoogle Scholar
  18. 18.
    Yousef JM, Danial EN. In vitro antibacterial activity and minimum inhibitory concentration of zinc oxide and nano-particle zinc oxide against pathogenic strains. J Health Sci. 2012;2(4):38–42.CrossRefGoogle Scholar
  19. 19.
    Li QL, Mahendra S, Lyon DY, Brunet L, Liga MV, Li D, et al. Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res. 2008;42(18):4591–602.CrossRefGoogle Scholar
  20. 20.
    Yamamoto O, Nakakoshi K, Sasamoto T, Nakagawa H, Miura K. Adsorption and growth inhibition of bacteria on carbon materials containing zinc oxide. Carbon. 2001;39(11):1643–51.CrossRefGoogle Scholar
  21. 21.
    Cao XL, Cheng C, Ma YL, Zhao CS. Preparation of silver nanoparticles with antimicrobial activities and the researches of their biocompatibilities. J Mater Sci Mater Med. 2010;21(10):2861–8.CrossRefGoogle Scholar
  22. 22.
    Lu Z, Rong KF, Li J, Yang H, Chen R. Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria. J Mater Sci Mater Med. 2013;24(6):1465–71.CrossRefGoogle Scholar
  23. 23.
    Ghosh S, Goudar VS, Padmalekha KG, Bhat SV, Indi SS, Vasan HN. ZnO/Ag nanohybrid: synthesis, characterization, synergistic antibacterial activity and its mechanism. RSC Adv. 2012;2(3):930–40.CrossRefGoogle Scholar
  24. 24.
    Lu WW, Liu GS, Gao SY, Xing ST, Wang JJ. Tyrosine-assisted preparation of Ag/ZnO nanocomposites with enhanced photocatalytic performance and synergistic antibacterial activities. Nanotechnology. 2008;19(44):445711.CrossRefGoogle Scholar
  25. 25.
    Shah AH, Manikandan E, Ahmed MB, Ganesan V. Enhanced bioactivity of Ag/ZnO nanorods-a comparative antibacterial study. J Nanomed Nanotechol. 2013;4(168):2.Google Scholar
  26. 26.
    Bazant P, Kuritka I, Hudecek O, Machovsky M, Mrlik M, Sedlacek T. Microwave-assisted synthesis of Ag/ZnO hybrid filler, preparation, and characterization of antibacterial poly(vinyl chloride) composites made from the same. Polym Compos. 2014;35(1):19–26.CrossRefGoogle Scholar
  27. 27.
    Sachot N, Castano O, Mateos-Timoneda MA, Engel E, Planell JA. Hierarchically engineered fibrous scaffolds for bone regeneration. J R Soc Interface. 2013;10(88):20130684.CrossRefGoogle Scholar
  28. 28.
    ISO 22196:2007 (E). Plastics—measurement of antimicrobial activity on plastics surfaces. Geneva, Switzerland: International standard, International Organization for Standardization; 2007.Google Scholar
  29. 29.
    Jones A. Killer plastics: antimicrobial additives for polymers. Plast Eng. 2008;64(8):34–40.Google Scholar
  30. 30.
    JIS Z 2801. Antimicrobial products—test for antimicrobial activity and efficacy. Tokyo, Japan: Japanese Standards Association, JIS Z 2801; 2000.Google Scholar
  31. 31.
    Torlak E, Sert D. Antibacterial effectiveness of chitosane-propolis coated polypropylene films against foodborne pathogens. Int J Biol Macromol. 2013;60:52–5.CrossRefGoogle Scholar
  32. 32.
    Bazant P, Kuritka I, Machovsky M, Sedlacek T, Pastorek M, editors. Microwave assisted synthesis of Ag–ZnO particles and their antibacterial properties. Mathematical methods and techniques in engineering and environmental science 4th WSEAS international conferences on material science; 3–5.11.2011; Catania, Italy; 2011.Google Scholar
  33. 33.
    Baruah S, Dutta J. Hydrothermal growth of ZnO nanostructures. Sci Technol Adv Mater. 2009;10(1):013001.CrossRefGoogle Scholar
  34. 34.
    Chazeau L, Cavaille JY, Canova G, Dendievel R, Boutherin B. Viscoelastic properties of plasticized PVC reinforced with cellulose whiskers. J Appl Polym Sci. 1999;71(11):1797–808.CrossRefGoogle Scholar
  35. 35.
    Cassie ABD, Baxter S. Wettability of porous surfaces. Trans Faraday Soc. 1944;40:546–51.CrossRefGoogle Scholar
  36. 36.
    Wenzel R. Resistance of solid surfaces to wetting by water. Ind Eng Chem. 1936;28(8):988–94.CrossRefGoogle Scholar
  37. 37.
    Extrand CW. Criteria for ultralyophobic surfaces. Langmuir. 2004;20(12):5013–8.CrossRefGoogle Scholar
  38. 38.
    Rush S, Mcfee R, Abildskov JA. Resistivity of body tissues at low frequencies. Circ Res. 1963;12(1):40–50.CrossRefGoogle Scholar
  39. 39.
    Latif I, Al-Abodi EE, Badri DH, Khafari JA. Preparation, characterization and electrical study of (carboxymethylated polyvinyl alcohol/ZnO) nanocomposites. Am J Polym Sci. 2012;2(6):135–40.CrossRefGoogle Scholar
  40. 40.
    Jasem SH, Hussain WA. Dielectric properties of carbon black/PVC (cement) composites. J Appl Polym Sci. 2012;38(1.A):60–70.Google Scholar
  41. 41.
    Geilich BM, Webster TJ. Reduced adhesion of Staphylococcus aureus to ZnO/PVC nanocomposites. Int J Nanomed. 2013;8:1177–84.Google Scholar
  42. 42.
    Marambio-Jones C, Hoek EMV. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res. 2010;12(5):1531–51.CrossRefGoogle Scholar
  43. 43.
    Kong H, Jang J. Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles. Langmuir. 2008;24(5):2051–6.CrossRefGoogle Scholar
  44. 44.
    Nair S, Sasidharan A, Rani VVD, Menon D, Nair S, Manzoor K, et al. Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. J Mater Sci Mater Med. 2009;20:235–41.CrossRefGoogle Scholar
  45. 45.
    Spathis P, Poulios I. The corrosion and photocorrosion of zinc and zinc-oxide coatings. Corros Sci. 1995;37(5):673–80.CrossRefGoogle Scholar
  46. 46.
    Yang ZM, Zhang P, Ding YH, Jiang Y, Long ZL, Dai WL. Facile synthesis of Ag/ZnO heterostructures assisted by UV irradiation: highly photocatalytic property and enhanced photostability. Mater Res Bull. 2011;46(10):1625–31.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Pavel Bazant
    • 1
  • Ivo Kuritka
    • 1
  • Lukas Munster
    • 1
  • Michal Machovsky
    • 1
  • Zuzana Kozakova
    • 1
  • Petr Saha
    • 1
  1. 1.Centre of Polymer Systems, University InstituteTomas Bata University in ZlínZlinCzech Republic

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