Bioactivity of porous biphasic calcium phosphate enhanced by recombinant human bone morphogenetic protein 2/silk fibroin microsphere

  • Liang Chen
  • Yong Gu
  • Yu Feng
  • Xue-Song Zhu
  • Chun-Zeng Wang
  • Hai-Long Liu
  • Hai-Yun Niu
  • Chi Zhang
  • Hui-Lin Yang


To prepare a bioactive bone substitute, which integrates biphasic calcium phosphate (BCP) and rhBMP-2/silk fibroin (SF) microsphere, and to evaluate its characteristics. Hydroxyapatite and β-tricalcium phosphate were integrated with a ratio of 60–40 %. RhBMP-2/SF (0.5 μg/1 mg) microsphere was prepared, and its rhBMP-2-release kinetics was assed. After joining pore-forming agent (Sodium chloride, NaCl), porous BCP/rhBMP-2/SF were manufactured, and its characteristics and bioactivity in vitro were evaluated. Mean diameter of rhBMP-2/SF microsphere was 398.7 ± 99.86 nm, with a loading rate of 4.53 ± 0.08 %. RhBMP-2 was released in a dual-phase pattern, of which fast-release (nearly half of protein released) focused on the initial 3 days, and slow-release sustained more than 28 days. With the increase in concentration of NaCl, greater was porosity and pore size, but smaller mechanical strength of BCP/rhBMP-2/SF. Material with 150 % (w/v) NaCl had an optimal performance, with a porosity of 78.83 %, pore size of 293.25 ± 42.77μm and mechanical strength of 31.03 MPa. Proliferation of human placenta-derived mesenchymal stem cells (hPMSCs) on leaching extract medium was similar to the normal medium (P = 0.89), which was better than that on control group (P = 0.03). Activity of alkaline phosphatase on BCP/rhBMP-2/SF surface was higher than on pure BCP at each time point except at 1 day (P < 0.05). RhBMP-2 has a burst release on early times and a sustaining release on later times. BCP/rhBMP-2/SF with 150 % (w/v) pore-forming agent has excellent porosity, pore size and mechanical strength. The biomaterial induces proliferation and differentiation hPMSCs effectively.


Silk Fibroin Burst Release Biphasic Calcium Phosphate Gelatin Hydrogel Silk Fibroin Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The study was financially supported by the National Natural Science Foundation of China (81071450, 81371930, 81171689 and 81301559), Natural Science Foundation of Jiang Su Province (BK2011264), Key Talented Man Project of Jiang Su Province (RC2011102), and Young Instructor Science Project of Soochow University (SDY2011A35).


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Liang Chen
    • 1
  • Yong Gu
    • 1
  • Yu Feng
    • 1
  • Xue-Song Zhu
    • 1
  • Chun-Zeng Wang
    • 1
  • Hai-Long Liu
    • 1
  • Hai-Yun Niu
    • 1
  • Chi Zhang
    • 1
  • Hui-Lin Yang
    • 1
  1. 1.Department of Orthopaedic SurgeryThe First Affiliated Hospital of Soochow UniversitySuzhouChina

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