Poly(2-substituted-2-oxazoline) surfaces for dermal fibroblasts adhesion and detachment

  • Andrzej Dworak
  • Alicja Utrata-Wesołek
  • Natalia Oleszko
  • Wojciech Wałach
  • Barbara Trzebicka
  • Jacek Anioł
  • Aleksander L. Sieroń
  • Agnieszka Klama-Baryła
  • Marek Kawecki


The thermoresponsive surfaces of brush structure (linear polymer chains tethered on the surface) based on poly(2-isopropyl-2-oxazoline)s and copolymers of 2-ethyl-2-oxazoline and 2-nonyl-2-oxazoline were obtained using the grafting-to method. The living oxazoline (co)polymers have been synthesized by cationic ring-opening polymerization and subsequently terminated by the reactive amine groups present on the surface. The changes in the surface morphology, philicity and thickness occurring during surface modification were monitored via atomic force microscopy, contact angle and ellipsometry. The thickness of the (co)poly(2-substituted-2-oxazoline) layers ranged from 4 to 11 nm depending on the molar mass of immobilized polymer and reversibly varied with the temperature changes. This confirmed thermoresponsive properties of obtained surfaces. The obtained polymer surfaces were used as a support for dermal fibroblast culture and detachment. The fibroblasts’ adhesion and proliferation on the polymer surfaces were observed when the culture temperature was above the cloud point temperature of the immobilized polymer. Lowering the temperature resulted in the detachment of the dermal fibroblast sheets from the polymer layers, which makes these surfaces suitable for the treatment of wounds and in skin tissue engineering.


Contact Angle Molar Mass Dermal Fibroblast Polymer Layer Cell Sheet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the European Union, European Regional Development Fund, project “DERMOSTIM” UDA-POIG.01.03.01-00-088/08. Part of this work was supported by the National Science Centre, the decision DEC-2012/07/N/ST5/00261. N.O. gratefully acknowledges European Social Fund within the project “Scholarships for the development of Silesia” for financial support. The authors thank Dr. Liliana Szyk-Warszynska from the Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences for performing the ellipsometry measurements.

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

10856_2013_5135_MOESM1_ESM.docx (241 kb)
Supplementary material 1 (DOCX 240 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Andrzej Dworak
    • 1
  • Alicja Utrata-Wesołek
    • 1
  • Natalia Oleszko
    • 1
  • Wojciech Wałach
    • 1
  • Barbara Trzebicka
    • 1
  • Jacek Anioł
    • 2
  • Aleksander L. Sieroń
    • 2
  • Agnieszka Klama-Baryła
    • 3
  • Marek Kawecki
    • 3
  1. 1.Center of Polymer and Carbon MaterialsPolish Academy of SciencesZabrzePoland
  2. 2.Department of General, Molecular Biology and GeneticsMedical University of SilesiaKatowicePoland
  3. 3.Center for Burn TreatmentSiemianowice SlaskiePoland

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