Long-term biocompatibility evaluation of 0.5 % zinc containing hydroxyapatite in rabbits
This study investigates the long-term biocompatibility of 0.5 % zinc-containing hydroxyapatite compared with hydroxyapatite. Spheres (425 < ∅ < 550) of both materials were produced by extrusion of ceramic slurry in calcium chloride and characterized by FTIR, XRD, XRF and SEM. Fifteen White New Zealand rabbits were submitted to general anesthesia, and an perforation (2 mm), was made in each tibia, one for zinc-containing hydroxyapatite sphere implantation and one for hydroxyapatite sphere implantation. After 26, 52 and 78 weeks, the animals were euthanized, and the fragment containing the biomaterial was harvested. A 30–50 μm section was obtained for histological analysis in bright field and polarized light. SEM images revealed similar morphologies between the tested biomaterials. Histological analysis showed that there was no difference between the test groups. The morphometric analysis, however, indicates that there was a greater absorption. The materials are biocompatible, promote osteogenesis and that the zinc-containing hydroxyapatite microspheres were absorbed more quickly.
KeywordsApatite Hydroxyapatite Energy Dispersive Spectroscopy Sodium Alginate Meloxicam
The authors acknowledge the financial support of FAPERJ, FINEP, DECIT-MS and CNPq. In addition to the research partners LNLS, COPPE, INMETRO and CBPF.
- 17.Barrere F, Van Blitterswijk CA, Groot KD. Bone regeneration: molecular and cellular interactions with calcium phosphate ceramics. Int J Nanomed. 2006;1:317–32.Google Scholar
- 18.de Lima IR, Alves GG, Soriano CA, Campaneli AP, Gasparoto TH, Ramos ES Jr, de Sena LÁ, Rossi AM, Granjeiro JM. Understanding the impact of divalent cation substitution on hydroxyapatite: an in vitro multiparametric study on biocompatibility. J Biomed Mater Res A. 2011;98:351–8.Google Scholar