Long-term biocompatibility evaluation of 0.5 % zinc containing hydroxyapatite in rabbits

  • Rodrigo F. B. ResendeEmail author
  • Gustavo V. O. Fernandes
  • Sílvia R. A. Santos
  • Alexandre M. Rossi
  • Inayá Lima
  • José M. Granjeiro
  • Mônica D. Calasans-Maia


This study investigates the long-term biocompatibility of 0.5 % zinc-containing hydroxyapatite compared with hydroxyapatite. Spheres (425 < ∅ < 550) of both materials were produced by extrusion of ceramic slurry in calcium chloride and characterized by FTIR, XRD, XRF and SEM. Fifteen White New Zealand rabbits were submitted to general anesthesia, and an perforation (2 mm), was made in each tibia, one for zinc-containing hydroxyapatite sphere implantation and one for hydroxyapatite sphere implantation. After 26, 52 and 78 weeks, the animals were euthanized, and the fragment containing the biomaterial was harvested. A 30–50 μm section was obtained for histological analysis in bright field and polarized light. SEM images revealed similar morphologies between the tested biomaterials. Histological analysis showed that there was no difference between the test groups. The morphometric analysis, however, indicates that there was a greater absorption. The materials are biocompatible, promote osteogenesis and that the zinc-containing hydroxyapatite microspheres were absorbed more quickly.


Apatite Hydroxyapatite Energy Dispersive Spectroscopy Sodium Alginate Meloxicam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge the financial support of FAPERJ, FINEP, DECIT-MS and CNPq. In addition to the research partners LNLS, COPPE, INMETRO and CBPF.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Rodrigo F. B. Resende
    • 1
    • 6
    Email author
  • Gustavo V. O. Fernandes
    • 1
  • Sílvia R. A. Santos
    • 2
  • Alexandre M. Rossi
    • 2
  • Inayá Lima
    • 3
  • José M. Granjeiro
    • 1
    • 4
    • 5
  • Mônica D. Calasans-Maia
    • 6
  1. 1.Cell and Molecular Biology Department, Biology InstituteFluminense Federal UniversityNiteroi, Rio de JaneiroBrazil
  2. 2.Biomaterials Laboratory-LABIOMATBrazilian Physics of Center ResearchesRio de JaneiroBrazil
  3. 3.Nuclear Instrumentation LaboratoryNuclear Engineering Program, COPPE/UFRJRio de JaneiroBrazil
  4. 4.Bioengineering DepartmentNational Institute of Metrology, Quality and TechnologyDuque de Caxias, Rio de JaneiroBrazil
  5. 5.Clinical Research UnitFluminense Federal UniversityNiteroi, Rio de JaneiroBrazil
  6. 6.Oral Surgery Department—Dentistry SchoolFluminense Federal UniversityNiteroi, Rio de JaneiroBrazil

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