Rapidly stopping hemorrhage by enhancing blood clotting at an opened wound using chitosan/polylactic acid/polycaprolactone wound dressing device
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Doxycycline and monosodium glutamate (MSG) loaded chitosan (CHI)/polylactic acid (PLA)/polycaprolactone (PCL) blend film was studied as a model device to deliver drug to targeted human organ which in this case was the skin with opened wound. The CHI/PLA/PCL blend film containing 60 % CHI, 28 % PLA, and 12 % PCL exhibited the good properties for making the dressing device. It was observed that doxycycline/MSG loaded CHI/PLA/PCL blend film could rapidly deliver both doxycycline and MSG at the high release percentage approaching 100 % loaded. MSG accelerated blood clotting and fibrin formation; thus, it exhibited the good hemostatic activity. The antibacterial activity of doxycycline loaded CHI/PLA/PCL blend film against Staphylococcus aureus and Escherichia coli as model bacteria was investigated. Doxycycline release played the crucial role in bacterial inhibition as observed from the lowest bacterial cell dry weight observed when compared with the control bacterial culture or the bacterial cultures with the presence of other films studied.
KeywordsDoxycycline Water Vapor Transmission Rate Fibrin Formation Monosodium Glutamate Phosphate Buffer Saline Buffer
This work has been supported by the Graduate School of Chulalongkorn University via The 90th Anniversary Of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund). Partial funding from the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (AM1026A-55) and Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project (CU56-AM05) is also acknowledged.
- 1.Kathryn EU, Scott MC, Robert SL, Kevin MS. Polymeric systems for controlled drug release. Chem Rev. 1999;9:3181–98.Google Scholar
- 7.Mershon MM. Compositions and methods for reducing blood and fluid loss from open wounds. United States Patent no. 7,303,759 Dec 2007.Google Scholar
- 9.Smith SA, Mutch NJ, Baskar D, Rohloff P, Docampo R, Morrissey JH. Polyphosphate modulates blood coagulation and fibrinolysis. Biochemistry. 2006;103:903–8.Google Scholar
- 11.Ahuja A, Martin DP, McCarthy SJ. Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from chitosan and including a polymer mesh material of poly-4-hydroxy butyrate. United States Patent no. US2007/0166387 A1 July 2007.Google Scholar
- 14.Tanveer AK, Peh KK, Ch’ng HS. Mechanical, bioadhesive strength and biological evaluation of Chitosan film for wound dressing. J Pharm Pharm Sci. 2000;3(3):303–11.Google Scholar
- 20.Bowen R. Vitamin K. 1999. http://abl.cumbs.colostate.edu/hbook/pathphysic/misc_topics/vitamink.html. Accessed 12 Dec 2006.
- 21.Higdon J. Vitamin K. 2004. http://lpi.oregoustate.edu/infocenter/vitamins/vitamink/.. Accessed 12 Dec 2006.
- 22.Tollefsen D. Blood coagulation. 2006. http://tollefsen.wustl.edu/projects/Coagulation/Coagulation.html. Accessed 9 Nov 2006.
- 24.Bi E, Lutkenhaus J. Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring. J Bacteriol. 1993;175:1118–25.Google Scholar
- 26.Gottesmann ME. Reaction of ribosome-bound peptidyl transfer ribonucleic acid with aminoacyl transfer ribonucleic acid or puromycin. J Biol Chem. 1967;242:5564.Google Scholar