Rapidly stopping hemorrhage by enhancing blood clotting at an opened wound using chitosan/polylactic acid/polycaprolactone wound dressing device



Doxycycline and monosodium glutamate (MSG) loaded chitosan (CHI)/polylactic acid (PLA)/polycaprolactone (PCL) blend film was studied as a model device to deliver drug to targeted human organ which in this case was the skin with opened wound. The CHI/PLA/PCL blend film containing 60 % CHI, 28 % PLA, and 12 % PCL exhibited the good properties for making the dressing device. It was observed that doxycycline/MSG loaded CHI/PLA/PCL blend film could rapidly deliver both doxycycline and MSG at the high release percentage approaching 100 % loaded. MSG accelerated blood clotting and fibrin formation; thus, it exhibited the good hemostatic activity. The antibacterial activity of doxycycline loaded CHI/PLA/PCL blend film against Staphylococcus aureus and Escherichia coli as model bacteria was investigated. Doxycycline release played the crucial role in bacterial inhibition as observed from the lowest bacterial cell dry weight observed when compared with the control bacterial culture or the bacterial cultures with the presence of other films studied.


Doxycycline Water Vapor Transmission Rate Fibrin Formation Monosodium Glutamate Phosphate Buffer Saline Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work has been supported by the Graduate School of Chulalongkorn University via The 90th Anniversary Of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund). Partial funding from the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (AM1026A-55) and Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project (CU56-AM05) is also acknowledged.


  1. 1.
    Kathryn EU, Scott MC, Robert SL, Kevin MS. Polymeric systems for controlled drug release. Chem Rev. 1999;9:3181–98.Google Scholar
  2. 2.
    Zhou S, Song B, Li X. In vitro degradation and release profiles for Poly-dl-lactide film containing paracetamol. J Mater Sci Mater Med. 2007;18:1623–6.CrossRefGoogle Scholar
  3. 3.
    Sahoo S, Sasmal A, Nanda R, Phani AR, Nayak PL. Synthesis of chitosan–polycaprolactone blend for control delivery of ofloxacin drug. Carbohydr Polym. 2009;79:106–13. doi: 10.1016/j.carbpol.2009.07.042.CrossRefGoogle Scholar
  4. 4.
    Chen CC, Chueh JY, Tseng H, Huang HM, Lee SY. Preparation and characterization of biodegradable PLA polymeric blends. Biomaterials. 2003;24:1167–73.CrossRefGoogle Scholar
  5. 5.
    Gentry PA. Comparative aspects of blood coagulation. Vet J. 2004;168:238–51.CrossRefGoogle Scholar
  6. 6.
    Urata J, Shojo H, Kaneko Y. Inhibition mechanisms of hematophagous invertebrate compounds acting on the host blood coagulation and platelet aggregation pathway. Biochime. 2003;85:493–500.CrossRefGoogle Scholar
  7. 7.
    Mershon MM. Compositions and methods for reducing blood and fluid loss from open wounds. United States Patent no. 7,303,759 Dec 2007.Google Scholar
  8. 8.
    Ruiz FA, Lea CR, Oldfield E, Docampo R. Human platelet dense granules contain polyphosphate and are similar to acidocalcisomes of bacteria and unicellular eukaryotes. J Biol Chem. 2004;279:44250–7.CrossRefGoogle Scholar
  9. 9.
    Smith SA, Mutch NJ, Baskar D, Rohloff P, Docampo R, Morrissey JH. Polyphosphate modulates blood coagulation and fibrinolysis. Biochemistry. 2006;103:903–8.Google Scholar
  10. 10.
    Wynd FL. A possible function of vitamin K in plants. Am Nat. 1944;78:59–67.CrossRefGoogle Scholar
  11. 11.
    Ahuja A, Martin DP, McCarthy SJ. Hemostatic compositions, assemblies, systems, and methods employing particulate hemostatic agents formed from chitosan and including a polymer mesh material of poly-4-hydroxy butyrate. United States Patent no. US2007/0166387 A1 July 2007.Google Scholar
  12. 12.
    Osoniyi O, Onajobi F. Coagulant and anticoagulant activities in Jatropha curcas latex. J Ethnopharmacol. 2003;89:101–5.CrossRefGoogle Scholar
  13. 13.
    Korsmeyer RC, Peppas NA. Effect of the morphology of hydrophilic polymeric matrices on the diffusion and release of water soluble drugs. J Membr ci. 1981;9:211–27.CrossRefGoogle Scholar
  14. 14.
    Tanveer AK, Peh KK, Ch’ng HS. Mechanical, bioadhesive strength and biological evaluation of Chitosan film for wound dressing. J Pharm Pharm Sci. 2000;3(3):303–11.Google Scholar
  15. 15.
    Queen D, Gaylor JDS, Ebans JH, Courtney JM. The preclinical evaluation of the water vapour transmission rate through burn wound dressing. Biomaterials. 1987;8:367–71.CrossRefGoogle Scholar
  16. 16.
    Boateng JS, Matthews KH, Stevens HNE, Eccleston GM. Wound healing dressings and drug delivery systems: a review. J Pharm Sci. 2008;97(8):2892–923.CrossRefGoogle Scholar
  17. 17.
    Wang QZ, Chen XG, Li ZX, Wang S, Liu CS, Meng XH, Liu CG, Lvm YH, Yu LJ. Preparation and blood coagulation evaluation of chitosan microspheres. J Mater Sci Mater Med. 2008;19:1371–7.CrossRefGoogle Scholar
  18. 18.
    Edwards JV, Howley P, Prevost N, Condon B, Arnold J, Diegelmann R. Positively and negatively charged ionic modifications to cellulose assessed as cotton-based protease-lowering and hemostatic wound agents. Cellulose. 2009;16:911–21.CrossRefGoogle Scholar
  19. 19.
    Schmidt AE, Stewart JE, Mathur A, Krishnaswamy S, Bajaj SP. Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa binding. J Mol Biol. 2005;350:78–91.CrossRefGoogle Scholar
  20. 20.
    Bowen R. Vitamin K. 1999. http://abl.cumbs.colostate.edu/hbook/pathphysic/misc_topics/vitamink.html. Accessed 12 Dec 2006.
  21. 21.
    Higdon J. Vitamin K. 2004. http://lpi.oregoustate.edu/infocenter/vitamins/vitamink/.. Accessed 12 Dec 2006.
  22. 22.
    Tollefsen D. Blood coagulation. 2006. http://tollefsen.wustl.edu/projects/Coagulation/Coagulation.html. Accessed 9 Nov 2006.
  23. 23.
    Vallapa N, Wiarachai O, Thongchul N, Pan JS, Tangpasuthadol V, Kiatkamjornwong S, Hoven VP. Enhancing antibacterial activity of chitosan surface by heterogeneous quaternization. Carbohy Polym. 2011;83:868–75.CrossRefGoogle Scholar
  24. 24.
    Bi E, Lutkenhaus J. Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring. J Bacteriol. 1993;175:1118–25.Google Scholar
  25. 25.
    Klemm P. Fimbrial adhesions of Escherichia coli. Rev Infect Dis. 1985;7:321–40.CrossRefGoogle Scholar
  26. 26.
    Gottesmann ME. Reaction of ribosome-bound peptidyl transfer ribonucleic acid with aminoacyl transfer ribonucleic acid or puromycin. J Biol Chem. 1967;242:5564.Google Scholar
  27. 27.
    Oehler R, Polacek N, Steiner G, Barta A. Interaction of tetracycline with RNA: photoincorporation into ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1997;25(6):1219–24.CrossRefGoogle Scholar
  28. 28.
    Anokhina MM, Barta A, Nierhaus KH, Spiridonova VA, Kopylov AM. Mapping of the second tetracycline binding site on the ribosomal small subunit of E. coli. Nucleic Acids Res. 2004;32(8):2594–7.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Wasinee Boonkong
    • 1
  • Amorn Petsom
    • 2
  • Nuttha Thongchul
    • 3
  1. 1.Program in Petrochemistry and Polymer Science, Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Research Center for Bioorganic Chemistry, Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Institute of Biotechnology and Genetic Engineering, Chulalongkorn UniversityBangkokThailand

Personalised recommendations