Rapidly stopping hemorrhage by enhancing blood clotting at an opened wound using chitosan/polylactic acid/polycaprolactone wound dressing device
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Doxycycline and monosodium glutamate (MSG) loaded chitosan (CHI)/polylactic acid (PLA)/polycaprolactone (PCL) blend film was studied as a model device to deliver drug to targeted human organ which in this case was the skin with opened wound. The CHI/PLA/PCL blend film containing 60 % CHI, 28 % PLA, and 12 % PCL exhibited the good properties for making the dressing device. It was observed that doxycycline/MSG loaded CHI/PLA/PCL blend film could rapidly deliver both doxycycline and MSG at the high release percentage approaching 100 % loaded. MSG accelerated blood clotting and fibrin formation; thus, it exhibited the good hemostatic activity. The antibacterial activity of doxycycline loaded CHI/PLA/PCL blend film against Staphylococcus aureus and Escherichia coli as model bacteria was investigated. Doxycycline release played the crucial role in bacterial inhibition as observed from the lowest bacterial cell dry weight observed when compared with the control bacterial culture or the bacterial cultures with the presence of other films studied.
KeywordsDoxycycline Water Vapor Transmission Rate Fibrin Formation Monosodium Glutamate Phosphate Buffer Saline Buffer
This work has been supported by the Graduate School of Chulalongkorn University via The 90th Anniversary Of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund). Partial funding from the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (AM1026A-55) and Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project (CU56-AM05) is also acknowledged.
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