Chitosan/bovine serum albumin co-micropatterns on functionalized titanium surfaces and their effects on osteoblasts



Chitosan (CS)/bovine serum albumin (BSA) micropatterns were prepared on functionalized Ti surfaces by micro-transfer molding (μ-TM). μ-TM realized the spatially controlled immobilization of cells and offered a new way of studying the interaction between micropatterns and cells. Two kinds of micropatterns were produced: (1) microgrooves representing a discontinuously grooved co-micropattern, with the rectangular CS region separated by BSA walls; (2) microcylinders representing a continuously interconnected co-micropattern, with the net-like CS region separated by BSA cylinders. A comparison of cell behaviors on the two types of micropatterns indicated that the shape rather than the size had a dominant effect on cell proliferation. The micropattern size in the same range of cell diameters favored cell proliferation. However, cell differentiation was more sensitive to the size rather than to the shape of the micropatterns. In conclusion, cell behavior can be regulated by micropatterns integrating different materials.


Bovine Serum Albumin Solution Bovine Serum Albumin Molecule Alamar Blue Assay PDMS Stamp Silicon Master 



This project was financially supported by the National Basic Research Program of China (973 Program, 2012CB933602), NSFC (31070851), Program for New Century Excellent Talents in University (NCET-10-0704), Sichuan Youth Science-Technology Foundation (2011JQ0010), Fundamental Research Funds for the Central Universities (SWJTU11CX150).


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Dan Li
    • 1
  • Xiong Lu
    • 1
  • Hong Lin
    • 1
  • Fuzeng Ren
    • 2
  • Yang Leng
    • 2
  1. 1.Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengduChina
  2. 2.Department of Mechanical EngineeringHong Kong University of Science and TechnologyHong KongChina

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