A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces
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Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Seri-DL-Alam)] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss® bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.
KeywordsTissue Culture Plastic Resazurin Reduction Biomimetic Coating Human Bone Marrow MSCs Bovine Bone Mineral
The authors would like to thank all colleagues at the Tissue Regeneration Department of the Twente University for the kind support, as well as Éva Juhász and Balázs Hegedűs for the help with time-lapse microscopy. This work was financially supported by the grants BIO_SURF from the National Office for Research and Technology (NKTH) and TÁMOP-4.2.1-IKUT from the National Development Agency (NFÜ).
- 1.Morita Y, Yamasaki K, Hattori K. A feasibility study for in vitro evaluation of fixation between prosthesis and bone with bone marrow-derived mesenchymal stem cells. Clin Biomech. (Bristol, Avon) 2010;25:829–34.Google Scholar
- 10.Gastaldi G, Asti A, Scaffino MF, Visai L, Saino E, Cometa AM, et al. Human adipose-derived stem cells (hASCs) proliferate and differentiate in osteoblast-like cells on trabecular titanium scaffolds. J Biomed Mater Res A. 2010;94:790–9.Google Scholar
- 16.Detsch R, Dieser I, Deisinger U, Uhl F, Hamisch S, Ziegler G, et al. Biofunctionalization of dispense-plotted hydroxyapatite scaffolds with peptides: quantification and cellular response. J Biomed Mater Res A. 2010;92:493–503.Google Scholar
- 24.Jafarian M, Eslaminejad MB, Khojasteh A, Abbas FM, Dehghan MM, Hassanizadeh R. Marrow-derived mesenchymal stem cells-directed bone regeneration in the dog mandible: a comparison between biphasic calcium phosphate and natural bone mineral. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105:e14–24.Google Scholar
- 29.Galli D, Benedetti L, Bongio M, Maliardi V, Silvani G, Ceccarelli G, et al. In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly-l-lysine-treated titanium-6-aluminium-4-vanadium. J Biomed Mater Res A. 2011;97:118–26.Google Scholar
- 30.Subbiahdoss G, Pidhatika B, Coullerez G, Charnley M, Kuijer R, van der Mei HC, et al. Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating. Eur Cell Mater. 2010;19:205–13.Google Scholar
- 32.Schliephake H, Scharnweber D, Dard M, Sewing A, Aref A, Roessler S. Functionalization of dental implant surfaces using adhesion molecules. J Biomed Mater Res B Appl Biomater. 2005;73:88–96.Google Scholar
- 33.Stadlinger B, Pilling E, Huhle M, Khavkin E, Bierbaum S, Scharnweber D, et al. Suitability of differently designed matrix-based implant surface coatings: an animal study on bone formation. J Biomed Mater Res B Appl Biomater. 2008;87:516–24.Google Scholar