Journal of Materials Science: Materials in Medicine

, Volume 23, Issue 11, pp 2773–2781 | Cite as

Effects of intermittent hydrostatic pressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture

  • Jae Young Jeong
  • So Hee Park
  • Ji Won Shin
  • Yun Gyeong Kang
  • Ki-Ho Han
  • Jung-Woog Shin


Without using biochemical agents, in this study, we sought to investigate the potential of controlling the differentiation of mesenchymal stem cells (MSCs) into a specific cell type through the use of 3D co-culturing and mechanical stimuli. MSCs and primary cultured chondrocytes were separately encapsulated into alginate beads, and the two types of beads were separated by a membrane. For the investigation a computer-controllable bioreactor was designed and used to engage intermittent hydrostatic pressure (IHP). Five different magnitudes (0.20, 0.10, 0.05, 0.02 MPa and no stimulation) of IHP were applied. The stimulation pattern was the same for all groups: 2 h/day for 7 days starting at 24 h after seeding; 2 and 15 min cycles of stimulating and resting, respectively. Biochemical (DNA and GAG contents), histological (Alcian blue), and RT-PCR (Col II, SOX9, AGC) analyses were performed on days 1, 5, 10, and 20. The results from these analyses showed that stimulation with higher magnitudes of IHP (≥0.10 MPa) were more effective on the proliferation and differentiation of co-cultured MSCs. Together, these data demonstrate the potential of using mechanical stimulation and co-culturing for the proliferation and differentiation of MSCs, even without biochemical agents.


Mechanical Stimulation Mechanical Stimulus Alcian Blue Alginate Bead Stem Cell Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the grants of Technology Innovation Program (10038667, Ministry of Knowledge Economy, ROK) and Priority Research Centers Program (2010-0020224, The Ministry of Education, Science and Technology).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jae Young Jeong
    • 1
  • So Hee Park
    • 1
  • Ji Won Shin
    • 1
  • Yun Gyeong Kang
    • 1
  • Ki-Ho Han
    • 2
  • Jung-Woog Shin
    • 1
    • 3
  1. 1.Department of Biomedical EngineeringInje UniversityGimhaeRepublic of Korea
  2. 2.School of Nano Engineering, Inje UniversityGimhaeRepublic of Korea
  3. 3.First Research Team/Institute of Aged Life Redesign/Cardiovascular and Metabolic Disease Center/UHRC, Inje UniversityGimhaeRepublic of Korea

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