Fabrication of biocompatible titanium scaffolds using space holder technique

Abstract

Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH1.924 and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300–600 μm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluations were carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent attachment and proliferation of G-292 cells to the highly porous scaffolds fabricated with titanium hydride and urea of this research.

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Acknowledgments

Kind cooperation of Mr. D. Shamaee who provided tools and instruments for fabrication of the scaffolds is greatly appreciated. Deputy of research of Sharif University of Technology is also thanked for continued support of Seed of Design and Accomplishment of New Processes for Production and Application of Advanced Materials.

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Correspondence to S. K. Sadrnezhaad.

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Dezfuli, S.N., Sadrnezhaad, S.K., Shokrgozar, M.A. et al. Fabrication of biocompatible titanium scaffolds using space holder technique. J Mater Sci: Mater Med 23, 2483–2488 (2012). https://doi.org/10.1007/s10856-012-4706-3

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Keywords

  • Pure Titanium
  • Titanium Powder
  • Pore Shape
  • Titanium Hydride
  • Space Holder