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Journal of Materials Science: Materials in Medicine

, Volume 23, Issue 9, pp 2141–2149 | Cite as

Injectable thermogelling chitosan for the local delivery of bone morphogenetic protein

  • Shaun W. McLaughlin
  • Zhanwu Cui
  • Trevor Starnes
  • Cato T. Laurencin
  • Ho-Man Kan
  • Qian Wu
  • Lakshmi S. Nair
Article

Abstract

The aim of the present study was to evaluate the in vivo biocompatibility of injectable thermo gelling chitosan–ammonium hydrogen phosphate solution (chitosan–AHP) and its efficacy to deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in a bioactive form. The thermogel showed a typical foreign body response upon subcutaneous implantation surrounded by a fibrous capsule. Even at 4 and 8 weeks post implantation, significant neutrophil infiltration was observed within the gel. Chitosan–AHP gel retained most of the loaded rhBMP-2 after a small initial release. The bioactivity of the released protein was demonstrated in vitro by the increase in alkaline phosphatase activity of mouse pre osteoblast cells (MC3T3-E1). Histological and micro-computed tomography (μCT) evaluation showed evidence of ectopic bone formation upon 4 μg/mL rhBMP-2 loaded chitosan–AHP injection. The study demonstrated a neutrophil mediated local tissue response to chitosan–AHP gel and its ability to encapsulate and maintain the bioactivity of rhBMP-2.

Keywords

Chitosan Fibrous Capsule Ectopic Bone Formation Ammonium Hydrogen Phosphate Local Tissue Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors greatly acknowledge the funding from USAMRMI -W81XWH-10-1-0653.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Shaun W. McLaughlin
    • 1
  • Zhanwu Cui
    • 2
  • Trevor Starnes
    • 3
  • Cato T. Laurencin
    • 2
    • 4
  • Ho-Man Kan
    • 2
  • Qian Wu
    • 5
  • Lakshmi S. Nair
    • 2
    • 4
  1. 1.School of MedicineUniversity of Connecticut Health CenterFarmingtonUSA
  2. 2.Department of Orthopeadic Surgery, Institute for Regenerative EngineeringUniversity of Connecticut Health CenterFarmingtonUSA
  3. 3.School of MedicineUniversity of VirginiaCharlottesvilleUSA
  4. 4.Department of Chemical, Materials and Biomolecular Engineering, Biomedical Engineering, Institute of Material ScienceUniversity of ConnecticutStorrsUSA
  5. 5.Department of Pathology and Laboratory MedicineUniversity of Connecticut Health CenterFarmingtonUSA

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