In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

  • A. Gala-García
  • M. B. H. Carneiro
  • G. A. B. Silva
  • L. S. Ferreira
  • L. Q. Vieira
  • M. M. Marques
  • R. D. Sinisterra
  • M. E. Cortes


This study assess the effects of bioceramic and poly(lactic-co-glycolic acid) composite (BCP/PLGA) on the viability of cultured macrophages and human dental pulp fibroblasts, and we sought to elucidate the temporal profile of the reaction of pulp capping with a composite of bioceramic of calcium phosphate and biodegradable polymer in the progression of delayed dentine bridge after (30 and 60 days) in vivo. Histological evaluation of inflammatory infiltrate and dentin bridge formation were performed after 30 and 60 days. There was similar progressive fibroblast growth in all groups and the macrophages showed viability. The in vivo study showed that of the three experimental groups: BCP/PLGA composite, BCP and calcium hydroxide (Ca(OH)2) dentin bridging was the most prevalent (90 %) in the BCP/PLGA composite after 30 days, mild to moderate inflammatory response was present throughout the pulp after 30 days. After 60 days was observed dentine bridging in 60 % and necrosis in 40 %, in both groups. The results indicate that understanding BCP/PLGA composite is biocompatible and by the best tissue response as compared to calcium hydroxide in direct pulp capping may be important in the mechanism of delayed dentine bridge after 30 and 60 days.


Hard Tissue Calcium Hydroxide Dental Pulp Biphasic Calcium Phosphate Mineral Trioxide Aggregate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Fundação de Amparo a Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento (CAPES and INCT -Nanobiofar/CNPq Brazilian agencies for financial support.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • A. Gala-García
    • 1
  • M. B. H. Carneiro
    • 2
  • G. A. B. Silva
    • 3
  • L. S. Ferreira
    • 4
  • L. Q. Vieira
    • 2
  • M. M. Marques
    • 4
  • R. D. Sinisterra
    • 5
  • M. E. Cortes
    • 1
  1. 1.Department of Restorative Dentistry, Dentistry FacultyUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil
  2. 2.Department of Imunology and Biochemistry, Science Biological Institute Universidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil
  3. 3.Department of Morphology, Science Biological InstituteUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil
  4. 4.Department of Restorative Dentistry, Dentistry FacultyUniversidade de São Paulo (USP)Belo HorizonteBrazil
  5. 5.Department of Chemistry, Exact Science InstituteUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil

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