In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite
- 365 Downloads
This study assess the effects of bioceramic and poly(lactic-co-glycolic acid) composite (BCP/PLGA) on the viability of cultured macrophages and human dental pulp fibroblasts, and we sought to elucidate the temporal profile of the reaction of pulp capping with a composite of bioceramic of calcium phosphate and biodegradable polymer in the progression of delayed dentine bridge after (30 and 60 days) in vivo. Histological evaluation of inflammatory infiltrate and dentin bridge formation were performed after 30 and 60 days. There was similar progressive fibroblast growth in all groups and the macrophages showed viability. The in vivo study showed that of the three experimental groups: BCP/PLGA composite, BCP and calcium hydroxide (Ca(OH)2) dentin bridging was the most prevalent (90 %) in the BCP/PLGA composite after 30 days, mild to moderate inflammatory response was present throughout the pulp after 30 days. After 60 days was observed dentine bridging in 60 % and necrosis in 40 %, in both groups. The results indicate that understanding BCP/PLGA composite is biocompatible and by the best tissue response as compared to calcium hydroxide in direct pulp capping may be important in the mechanism of delayed dentine bridge after 30 and 60 days.
KeywordsHard Tissue Calcium Hydroxide Dental Pulp Biphasic Calcium Phosphate Mineral Trioxide Aggregate
The authors thank Fundação de Amparo a Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento (CAPES and INCT -Nanobiofar/CNPq Brazilian agencies for financial support.
- 5..Minouei H, Meratian M, Fathi M H, Ghazvinizadeh H. Biphasic calcium phosphate coating on cobalt-base surgical alloy during investment casting. J Mater Sci: Mater Med 2011; doi: 10.1007/s10856-011-4433-1.
- 6.Yin X, Stott MJ, Rubio A. Alpha and beta-tricalcium phosphate: a density functional study. Phys Rev B. 2003;68:205–20.Google Scholar
- 8.Zhang W, Walboomers XF, Jansen JA. The formation of tertiary dentin after pulp capping with calcium phosphate cement loaded with PLGA microparticles containing TGF-beta1. J Biomed Mater Res A. 2008;85:439–44.Google Scholar
- 9.Bhatnagar R, Li S. Biomimetic scaffolds for tissue engineering. Conf Proc IEEE Eng Med Biol Soc. 2004;7:5021–3.Google Scholar
- 17.American Society for Testing and Materials: Annual Book of ASTM Standards 1992: Medical Devices. ASTM, West Conshohocken.Google Scholar
- 23.Ignjatović N, Ninkov P, Kojić V, Bokurov M, Srdić V, Krnojelac D, Selaković S, Uskoković D. Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. Microsc Res Tech. 2006;69:976–82.CrossRefGoogle Scholar
- 25.Kim SS, Vacanti JP. The current status of tissue engineering as potential therapy. Semin Pediatr Surg. 1999;8:119–23.Google Scholar
- 36.Oliveira Mendes ST, Ribeiro Sobrinho AP, Carvalho AT, Souza Cortes MI, Vieira LQ In vitro evaluation of the cytotoxicity of two root canal sealers on macrophage activity. J Endod 2003;29:95–99.Google Scholar
- 38.Alcaide M, Serrano MC, Pagani R, Sánchez-Salcedo S, Nieto A, Vallet-Regí M, Portolés MT. L929 fibroblast and Saos-2 osteoblast response to hydroxyapatite-betaTCP/agarose biomaterial. J Biomed Mater Res A. 2009;89:539–49.Google Scholar
- 39.Botelho CM, Brooks RA, Best SM, Lopes MA, Santos JD, Rushton N, Bonfield W. Human osteoblast response to silicon-substituted hydroxyapatite. J Biomed Mater Res A. 2006;79:723–30.Google Scholar