Siloxane-poly(lactic acid)-vaterite composites with 3D cotton-like structure
- 694 Downloads
Trace amounts of ionic calcium and silicon species have been reported to stimulate the proliferation, differentiation, and mineralization of bone-forming cells. Composite materials comprising siloxane-doped calcium carbonate (vaterite) particles and poly(l-lactic acid) have been developed [siloxane-poly(lactic acid)-vaterite hybrid-composite, SiPVH] so far; they were designed such that calcium and silicate ions are gradually released from SiPVH and they show the chronic effects of ions on cellular activities. In the present work, SiPVH with a 3D cotton-like structure was prepared by electrospinning to obtain the major advantages of excellent bioactivity and ease of handling for bone filling surgery. The diameter of the fibrous skeletons that form structure of the cotton-like SiPVH was controlled to ~10 μm to achieve cellular migration into the spaces between fibers. The resulting cotton-like SiPVH showed good flexibility. The fiber surface was coated rapidly with numerous particles of several hundred nanometers in size by alternate soaking in CaCl2 and Na2HPO4. The treated cotton-like material, which released calcium and silicate ions gradually, showed good cellular migration behavior into the 3D structure in cell culture tests using murine osteoblast-like MC3T3-E1 cells.
KeywordsPLLA Vaterite Amorphous Calcium Phosphate Electrospinning Method Sodium Hydrogen Phosphate
The present work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Nos. 20390497 and 21700487) and a grant from Institute of Ceramics Research and Education, NITech.
- 17.Nakamura J, Maeda H, Obata A, Kasuga T. Private Communication, Structure of siloxane-containing vaterite particles and their dissolution behavior. In: Preprints of 24th Fall Meeting of The Ceramic Society of Japan. Tokyo: The Ceramic Society of Japan: 2011. p. 10 (1A27).Google Scholar
- 20.Takeuchi N, Machigashira M, Yamashita D, Shirakata Y, Kasuga T, Noguchi K, Ban S. Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration. Dental Mater J. in press.Google Scholar
- 26.Zhang YZ, Su B, Venugopal J, Ramakrishna S, Lim CT. Biomimetic and bioactive nanofibrous scaffolds from electrospun composite nanofibers. Int J Nanomedicine. 2007;2:623–38.Google Scholar
- 27.Fujikura K, Obata A, Lin S, Jones JR, Law RV, Kasuga T. Preparation of electrospun poly(lactic acid)-based hybrids containing siloxane-doped vaterite particles for bone regeneration, J Biomater Sci. in press (doi: 10.1163/092050611X582867).
- 28.Schneider OD, Loher S, Brunner TJ, Uebersax L, Simonet M, Grass RN, Merkle HP, Stark WJ. Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells. J Biomed Mater Res B Appl Biomater. 2008;84B:350–62.CrossRefGoogle Scholar
- 29.Fujikura K, Obata A, Kasuga T. Cellular migration to electrospun poly(lactic acid) fibermats. J Biomater Sci. in press (doi: 10.1163/092050611X599328).