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Preparation of injectable auto-forming alginate gel containing simvastatin with amorphous calcium phosphate as a controlled release medium and their therapeutic effect in osteoporosis model rat

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Abstract

Highly soluble amorphous calcium phosphate powder (ACP) was added to the alginate gel as a buffering agent, in an attempt to enable widely controlled release while avoiding an acidification of the gel-environment. Therapeutic effects of the ACP-containing alginate gel which slowly releases a drug, simvastatin, on osteoporosis model rats were examined. A model drug, simvastatin, incorporated in the alginate gel with ACP up to 0.5%, was continuously released for a long time under the acidic condition. The release rate was controlled by the amount of ACP, serving as a buffer to suppress acidity. When the alginate solution intramuscularly injected in the rat, a soft gel was formed in the injected site. Simvastatin released from the gel containing 0.5% of ACP showed high therapeutic effect on osteoporosis rat. Thus, the present injectable long-sustained release system is expected to be a novel drug delivery controlling device.

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Acknowledgments

This work was partly supported by the Japan Society for the Promotion of Science (nos. 21700494).

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Author notes

  1. Tomohiro Uchino

    Present address: College of Engineering, Nihon University, Fukushima, Japan

  2. Mamoru Senna

    Present address: RIKEN Innovation Center, Saitama, Japan

Authors and Affiliations

  1. Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan

    Tomoko Ito, Tomohiro Uchino, Mamoru Senna & Makoto Otsuka

  2. Faculty of Pharmaceutical Sciences, Musashino University, Tokyo, Japan

    Mami Saito & Makoto Otsuka

  3. Dipartimento di Scienze Mediche, Università del Piemonte Orientale, Novara, Italy

    Michele Iafisco, Maria Prat & Lia Rimondini

  4. Dipartimento di Chimica “G. Ciamician”, Alma Mater Studiorum, Università di Bologna, Bologna, Italy

    Michele Iafisco

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  1. Tomoko Ito
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  8. Makoto Otsuka
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Correspondence to Makoto Otsuka.

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Ito, T., Saito, M., Uchino, T. et al. Preparation of injectable auto-forming alginate gel containing simvastatin with amorphous calcium phosphate as a controlled release medium and their therapeutic effect in osteoporosis model rat. J Mater Sci: Mater Med 23, 1291–1297 (2012). https://doi.org/10.1007/s10856-012-4597-3

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  • DOI: https://doi.org/10.1007/s10856-012-4597-3

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